Tissue culture seedling raising method of nervilia fordii

A technology for tissue culture and S. japonica, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seedling rate and difficulty in artificial breeding of S. spp. Effect

Inactive Publication Date: 2015-11-18
莫玉明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: the purpose of the invention is to provide a method for raising seedlings by tissue culture for the difficulty of artificial breeding in the prior art and the problem that the seedling r

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 A kind of blue skyflower tissue culture raises seedlings, and its concrete steps are as follows:

[0017] 1. Cultivation of sterile seedlings: Soak and sterilize the blue skyflower bulbs in 0.2% mercuric solution for 15 minutes, soak them in sterile water on an ultra-clean workbench for 5 times, each time for 3 minutes, and then sow the bulbs In the culture medium, cultured under natural diffused light in the room;

[0018] 2. Induction medium: use MS as the basic medium, add phytohormones for induction culture, phytohormones use benzylpurine (BA) and naphthalic acid (NAA), the concentration of benzylpurine (BA) is 1.0mg / L, and Nai The concentration of acetic acid (NAA) is 0.1mg / L, and 2.5% sucrose and 0.7% agar are added at the same time, the temperature of the medium is kept at 24°C, and the pH value is adjusted to 6.0;

[0019] 3. Cluster bud induction: when the aseptic seedlings cultivated in step 1 grow to 5 cm, cut the top 2.5 cm long terminal bud ste...

Embodiment 2

[0023] Example 2 : a kind of blue skyflower tissue culture seedling cultivation, comprising the specific steps of accepting:

[0024] 1. Cultivation of sterile seedlings: Soak and sterilize the blue skyflower bulbs in 0.2% mercuric chloride solution for 16 minutes, soak them with sterile water on an ultra-clean workbench for 6 times, each time for 4 minutes, and then sow the bulbs In the culture medium, cultured under natural diffused light in the room;

[0025] 2. Induction medium: use MS as the basic medium, add phytohormones for induction culture, phytohormones use benzylpurine (BA) and naphthalic acid (NAA), the concentration of benzylpurine (BA) is 2.0mg / L, Nai The concentration of acetic acid (NAA) was 0.2mg / L, and 2.5% sucrose and 0.7% agar were added at the same time, the temperature of the medium was kept at 25°C, and the pH value was adjusted to 6.3;

[0026] 3. Cluster bud induction: When the aseptic seedlings cultivated in step 1 grow to 5-6 cm, cut the top 2.8 ...

Embodiment 3

[0030] Example 3 A kind of blue skyflower tissue culture raises seedlings, and its concrete steps are as follows:

[0031] 1. Cultivation of sterile seedlings: Soak and sterilize the blue skyflower bulbs in 0.2% mercuric chloride solution for 18 minutes, soak them with sterile water on an ultra-clean workbench for 7 times, each time for 5 minutes, and then sow the bulbs In the culture medium, cultured under natural diffused light in the room;

[0032] 2. Induction medium: use MS as the basic medium, add phytohormones for induction culture, phytohormones use benzylpurine (BA) and naphthalic acid (NAA), the concentration of benzylpurine (BA) is 2.5mg / L, Nai The concentration of acetic acid (NAA) is 0.3mg / L, and 2.5% sucrose and 0.7% agar are added at the same time, the temperature of the medium is kept at 26°C, and the pH value is adjusted to 6.5;

[0033] 3. Cluster bud induction: When the aseptic seedlings cultivated in step 1 grow to 6 cm, cut the top 3.0 cm long terminal ...

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PUM

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Abstract

Aiming at the problems that the artificial raising of nervilia fordii is large in difficulty and low in percentage of seedling emergency, the invention provides a tissue culture seedling raising method of nervilia fordii, belonging to the technical field of cultivation of traditional Chinese medicinal materials. The tissue culture seedling raising method comprises specific steps of culturing aseptic seedlings, inducing the medium, inducing clustered shoots, carrying out subculture multiplication on the clustered shoots, inducing rooting, and transplanting test-tube plantlets. With the method, the problem that the in the prior art, the artificial raising of nervilia fordii is large in difficulty and low in percentage of seedling emergency is solved. The tissue culture seedling raising method has the beneficial effects that the materials are easily available, the clustered shoot induction rate can achieve 50%-80%, the seedling emergency ratio can achieve 85% or above, the demand for large-quantity artificial cultivating is met, and through evaluation by adopting the thin-layer chromatography, the test-tube plantlets cultured by the method have the active ingredients consistent with those of the wild nervilia fordii.

Description

technical field [0001] The invention relates to the cultivation technology of Chinese herbal medicinal materials, in particular to a method for raising seedlings of blue skyflower, in particular to a method for growing seedlings of blue skyflower through tissue culture, and belongs to the technical field of cultivation of Chinese medicinal materials. Background technique [0002] Nervilia fordii, aliased as snipe genus, pearl grass, and pseudogastrodia, belongs to the family Orchidaceae, and its scientific name is Nerviliafordii (Hance) Schltr. It is mainly produced in Guangxi, Guangdong, Yunnan and other places, and it is distributed in the north, west and southwest of Guangxi. Qingtiankui is cold in nature, sweet in taste, moistens the lungs and relieves cough, clears heat and detoxifies, dissipates blood stasis and reduces swelling. Flavonoids, organic acids, phenols, sugars, polysaccharides, proteins, coumarin and lactones and other compounds. Pharmacological tests hav...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 莫玉明
Owner 莫玉明
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