The invention discloses externally-applied detoxification ointment for treatment of poisonous snake bite wound and a preparation method thereof, the externally-applied detoxification ointment comprises the following components by mass: 1-3 parts of aristolochia tubiflora, 1-3 parts of paris polyphylla, 1-3 parts of semiaquilegia adoxoides, 1-3 parts of herba portulacae, 1-3 parts of mosla cavaleriei L, 1-3 parts of marsh marigold, 1-3 parts of duchesnea, 1-3 parts of radix zanthoxyli, 1-3 parts of alternanthera sessilis, 1-3 parts of turkey corn, 1-3 parts of daphniphyllum calycinum, 1-3 parts of eomecon chionantha, 1-3 parts of sixangular dysosma rhizome, 1-3 parts of stephania japonica, 1-3 parts of polygonum perfoliatum L, 1-3 parts of radix cocculi and appropriate amount of wine and vinegar; the preparation method of the ointment includes the following steps: A, according to the mass ratio, selecting the good-quality aristolochia tubiflora, paris polyphylla, semiaquilegia adoxoides, herba portulacae, mosla cavaleriei L, marsh marigold, duchesnea, radix zanthoxyli, alternanthera sessilis, turkey corn, daphniphyllum calycinum, eomecon chionantha, sixangular dysosma rhizome, stephania japonica, polygonum perfoliatum L and radix cocculi to grind into powder respectively; B, filtering the ground powder with a separation sieve; C, spreading, according to the thickness of 6mm to 7mm, the well mixed powder in an ultraviolet sterilizing box for sterilization for stand-by use; and D, filling the appropriate amount of wine and vinegar into a stirring vessel in an asepsis room.