Method for preparing polysaccharide-ADH derivative by means of A-group meningococcal refined sugar

A meningococcal,-ADH technology, applied in the field of preparation of polysaccharide-ADH derivatives, can solve the problems of unfavorable protection of human health and the environment, achieve better derivation effect, ensure human health, and increase the effect of ADH derivation rate

Inactive Publication Date: 2015-11-18
CHENGDU OLYMVAX BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the use of highly toxic chemical reagents such as cyanogen bromide in the vaccine manufacturing process in the prior art is not conducive

Method used

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  • Method for preparing polysaccharide-ADH derivative by means of A-group meningococcal refined sugar

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The method for preparing polysaccharide-ADH derivatives by utilizing group A meningococcal refined sugar comprises the following steps:

[0026] (1) Dissolve meningococcal refined sugar in water for injection to prepare a polysaccharide solution with a concentration of 5 mg / ml, and adjust the pH to 9.0 with sodium hydroxide;

[0027] (2) Dissolve CDAP with acetonitrile to form a CDAP solution with a concentration of 100mg / ml; then add CDAP solution to the polysaccharide solution according to the weight ratio of polysaccharide:CDAP=1:0.5, and stir at room temperature for 2-5 minutes to make a CDAP mixture ;

[0028] (3) Add ADH with 0.2mol / L NaHCO 3 Dissolve into an ADH solution with a concentration of 100mg / ml, add ADH solution to the CDAP mixture according to the weight ratio of polysaccharide: ADH=1:3.5, and stir at room temperature for 1 hour;

[0029] (4) Finally, filter with ultrafiltration membrane bag. The ultrafiltrate used in ultrafiltration membrane bag filt...

Embodiment 2

[0031] The only difference between this example and Example 1 is that the weight ratio of polysaccharide and CDAP in step (2) in this example is 1:0.6; the weight ratio of polysaccharide and ADH in step (3) is 1:4.

Embodiment 3

[0033] The difference between this embodiment and embodiment 1 is only that the proportion and concentration of each chemical substance in this embodiment are added to the reaction, and the specific settings are as follows:

[0034] The concentration of the polysaccharide solution in step (1) is 10 mg / ml, and the pH is adjusted to 9.0 with sodium hydroxide;

[0035] The concentration of CDAP solution in step (2) is 80mg / ml, polysaccharide:CDAP=1:0.7;

[0036] The concentration of ADH solution in step (3) is 80mg / ml ADH solution, polysaccharide: ADH=1:5.

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Abstract

The invention discloses a method for preparing a polysaccharide-ADH derivative by means of A-group meningococcal refined sugar. The method comprises the following steps that 1, meningococcal refined sugar and water for injection are mixed to be prepared into a polysaccharide solution, and pH is adjusted to be 9.0; 2, CDAP is dissolved into a CDAP solution through acetonitrile, the CDAP solution is added to the polysaccharide solution according to the weight proportion: polysaccharide:CDAP=1:0.4-0.6, stirring is carried out for 2-5 minutes at room temperature, and CDAP mixed liquid is prepared; 3, ADH is dissolved into an ADH solution with the concentration of 100 mg/ml through NaHCO3 with the concentration of 0.2 mol/L, the ADH solution is added to the CDAP mixed liquid according to the weight proportion: polysaccharide:ADH=1:3-5, and stirring is carried out for one hour at room temperature; 4, finally, filtering and freeze drying are carried out through an ultrafiltration membrane. The method has the advantages of being environmentally friendly, safe, good in derivation effect and the like.

Description

technical field [0001] The invention relates to a preparation method of polysaccharide-ADH derivatives, in particular to a method for preparing polysaccharide-ADH derivatives by using the refined sugar of group A meningococcus. Background technique [0002] Meningococcal meningitis (referred to as meningitis, the same below) is an acute respiratory infectious disease caused by Neisseria meningitidis. For more than one hundred years, it has been popular or sporadic all over the world. It can cause sepsis and meningitis after infection with pathogenic bacteria. The susceptible population is mainly children, and the case fatality rate of fulminant type is the highest, which can reach 40% to 60%. The incidence rate in all continents of the world today is 1 / 100,000 to 10 / 100,000, and the total case fatality rate is 5% to 15%. Up to 20% of meningitis patients will have neurological sequelae, including intellectual impairment and deafness. Serological classification according to ...

Claims

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Application Information

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IPC IPC(8): A61K39/095A61K47/48A61P31/04
CPCA61K39/095
Inventor 吴强伍长华谭勇
Owner CHENGDU OLYMVAX BIOPHARM
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