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Mulberry red pigment extraction and purification method

A red pigment and mulberry technology, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of low extraction efficiency, complicated and confusing method descriptions, etc.

Inactive Publication Date: 2015-11-18
胡伟民
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Problems solved by technology

[0004] At present, most of the research on mulberry red pigment is focused on the optimization of the crude extraction process and the stability of the crude extraction pigment. The theory and technology are relatively mature, but the description of the specific method is complicated and confusing, and the extraction efficiency is also very low, and there are few studies on it. The whole process of extraction, purification and identification is unified and integrated

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  • Mulberry red pigment extraction and purification method

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Embodiment Construction

[0041] Below in conjunction with embodiment, further set forth the present invention. In the following detailed description, certain exemplary embodiments of the invention are described by way of illustration only. Needless to say, those skilled in the art would realize that the described embodiments can be modified in various different ways, all without departing from the spirit and scope of the present invention. Accordingly, the description is illustrative in nature and not intended to limit the scope of the claims.

[0042] The method for extracting and purifying mulberry red pigment comprises the following steps:

[0043] Step 1. Extraction process of mulberry red pigment

[0044] a1. Thaw frozen fresh mulberries naturally, grind them to form a feed liquid, use 80% ethanol and 0.1% HCl to configure the extract for leaching, the ratio of the two is 1:1, and add the extract to the feed liquid , sonicated at room temperature, filtered to obtain a red liquid;

[0045]b1. ...

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Abstract

The invention discloses a mulberry red pigment extraction and purification method. The method comprises the following four steps: extracting mulberry red pigment, determining the using amount of macroporous resin D101 in the purification technology, determining the yield, and using HPLC to measure the components. In the experiment to measure the mulberry red pigment, the maximum absorption wavelength is 520 nm. Through the research on using amount of extraction solvent, ultrasonic time, ultrasonic extraction frequency, and using amount of resin, the optimal technology is obtained: the extraction solvent is a mixture composed of ethanol (80%) and HCl according to a ratio of 1:1, the liquid / material ratio is 1:15, the ultrasonic time is 30 minutes, the extraction and ultrasonic treatment are carried out for two times, and the using amount of macroporous resin D101 is 2.7 g / g; and the yield of mulberry red pigment is 5.6 mg / g(mulberry). Through the HPLC, people acknowledges that the main component of mulberry red pigment is anthocyanin; the mulberry anthocyanin has a similar structure as that of huckleberry anthocyanin; and the mulberry anthocyanin can be used as a good raw material of healthcare products.

Description

technical field [0001] The invention relates to the technical field of pigment extraction and purification, in particular to a method for extracting and purifying mulberry red pigment. Background technique [0002] Antioxidant active substances are attracting more and more attention in modern life, especially the antioxidant, anti-aging, anti-ulcer, anti-inflammatory and anti-cancer products made from bilberry and blueberry in Japan and wolfberry in China. There are many health products for eliminating eye fatigue and increasing eyesight. In fact, mulberries are rich in sugars, organic acids, free amino acids, vitamins and trace elements, especially anti-oxidant active substances, which are comparable to lingonberry, blueberry and wolfberry. [0003] Mulberry, also known as mulberry fruit, black mulberry, and mulberry jujube, has juicy flesh and sweet taste. It is a treasure among fruits and is distributed in both north and south of my country. "Compendium of Materia Medic...

Claims

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Application Information

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IPC IPC(8): C09B61/00C07H17/065C07H1/08
CPCC09B61/00C07H1/08C07H17/065
Inventor 胡伟民任培华范兆乾宋喜云
Owner 胡伟民
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