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Method for producing (S)-biphenylylmethylcarbinol from black mold

A technology of black mold and biphenyl is applied in the field of black mold cell biocatalysis to prepare chiral pharmaceutical intermediate-1-ethanol, which can solve the problems of expensive coenzyme, addition, difficult industrial production and the like

Inactive Publication Date: 2015-11-18
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods generally have serious defects such as low conversion rate, long reaction time, need to add expensive coenzymes, and high cost, making it difficult to carry out industrial production

Method used

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  • Method for producing (S)-biphenylylmethylcarbinol from black mold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The ingredients of the seed medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, yeast extract 10g / L, glucose 20g / L, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L L, potassium dioxyphosphate 1.8-2.3g / L; add 15g / L agar powder when preparing solid medium.

[0021] The ingredients of the culture medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, soybean powder 3-5g / L, yeast extract 6-9g / L, glucose 18-22g / L, malt extract 25- 30g, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L, potassium dioxyphosphate 1.8-2.3g / L, calcium carbonate 0.7g / L, ferrous sulfate 0.18g / L, manganese sulfate 0.025g / L;pH5.3.

[0022] The preparation process of wet black mold cells is as follows: the black mold ATCC34956 was cultured on inclined planes and shake flasks to obtain seed liquid. Add culture medium to 1L fermenter; add culture medium to fermenter, autoclave at 121°C for 30 minutes, cool to 26-27°C, inoculate the bl...

Embodiment 2

[0025] The ingredients of the seed medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, yeast extract 10g / L, glucose 20g / L, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L L, potassium dioxyphosphate 1.8-2.3g / L; add 15g / L agar powder when preparing solid medium.

[0026] The ingredients of the culture medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, soybean powder 3-5g / L, yeast extract 6-9g / L, glucose 18-22g / L, malt extract 25- 30g, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L, potassium dioxyphosphate 1.8-2.3g / L, calcium carbonate 0.7g / L, ferrous sulfate 0.18g / L, manganese sulfate 0.025g / L;pH5.3.

[0027] The preparation process of wet black mold cells is as follows: the black mold ATCC34956 was cultured on inclined planes and shake flasks to obtain seed liquid. Add culture medium to a 5L fermenter; add culture medium to a fermenter, autoclave at 121°C for 30 minutes, cool to 26-27°C, inoculate bl...

Embodiment 3

[0030] The ingredients of the seed medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, yeast extract 10g / L, glucose 20g / L, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L L, potassium dioxyphosphate 1.8-2.3g / L; add 15g / L agar powder when preparing solid medium.

[0031]The ingredients of the culture medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, soybean powder 3-5g / L, yeast extract 6-9g / L, glucose 18-22g / L, malt extract 25- 30g, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L, potassium dioxyphosphate 1.8-2.3g / L, calcium carbonate 0.7g / L, ferrous sulfate 0.18g / L, manganese sulfate 0.025g / L;pH5.3.

[0032] The preparation process of the wet black mold cell is as follows: the black mold ATCC34956 is cultured on an inclined plane and a shaker flask to obtain a seed solution. Add culture medium to 10L fermenter; add culture medium to fermenter, autoclave at 121°C for 30 minutes, cool to 26-27°C, inocul...

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Abstract

The invention relates to a method for preparing (S)-1-(biphenyl-4-yl)ethanol from cells of black mold through biological catalysis. The method comprises the steps of adding a phosphate buffer solution into a reaction tank, and carrying out biological catalysis reaction on the cells of black mold. The method has the beneficial effects that the product yield is high, and the enantiomeric excess rate is high; and the production difficulties of existing methods are solved.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical engineering, and in particular relates to the technology of preparing chiral pharmaceutical intermediate (S)-1-(biphenyl-4-yl)ethanol by biocatalysis of black mold cells. Background technique [0002] Chiral (S)-1-(biphenyl-4-yl)ethanol is an important intermediate in the synthesis of chiral drugs, fine chemicals, pesticides and other special materials. Biocatalytic asymmetric reactions have the advantages of environmental friendliness, mild conditions, and high selectivity. As a green and efficient way to prepare chiral compounds, they are increasingly used in the production of some high value-added chiral compounds. Biocatalytic preparation of chiral (S)-1-(biphenyl-4-yl)ethanol has a good application prospect. [0003] Biocatalysis has outstanding advantages such as high catalytic efficiency, strong selectivity, mild conditions, and environmental friendliness. It is an important method ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22
Inventor 刘均洪王丽红梁方方
Owner QINGDAO UNIV OF SCI & TECH