Vaginal substrate material and preparation method thereof
A matrix material and vaginal technology, applied in the field of vaginal matrix material and its preparation, can solve the problems of biological activity and biomechanical properties, and the treatment effect needs to be further improved, and achieve low immunogenicity and cytotoxicity, biocompatibility and biological The effect of good mechanical properties and good application selection
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Embodiment 1
[0053] Preparation of porcine vaginal matrix material:
[0054] (1) Take the vaginal tissues of healthy adult pigs (the warm ischemia time is less than 30 minutes), and wash the obtained vaginal tissues repeatedly with sterile saline. The knife and surgical scissors cut and separate the outer membrane layer, muscular layer and mucous layer of the vaginal tissue. When tissue folds are encountered, the manipulator is used to peel off. Repeatedly, the outer membrane layer and muscular layer of the vaginal tissue are stripped and removed. A vaginal mucosal layer with no damage and uniform thickness was collected. Then cut into appropriate size, such as width 7.56±1.42cm, length 11.63±1.84cm, thickness 0.067±0.014cm, rinse for later use.
[0055] (2) Shake the vaginal mucosa layer treated in step (1) with sterile deionized water at 4°C for 48 hours, and the shaking speed is 130rpm;
[0056] The sterile deionized water used contained 0.1 mM / L of protease inhibitor phenylmethylsulf...
experiment example 1
[0074] Experimental Example 1: Detection and evaluation of the biological properties of the materials prepared in Example 1 and Comparative Example 1:
[0075] In this experimental example, the biological performance of the vaginal matrix material prepared in Example 1 of the present invention was tested and evaluated through Test 1 to Test 6, and compared with the performance of the acellular small intestinal submucosa matrix prepared in Comparative Example 1.
[0076] Since the outer 1 / 3 of the vagina (that is, the lower 1 / 3 of the vagina) and the 2 / 3 of the vaginal inner segment (that is, the upper 2 / 3 of the vagina) are differentiated from different germ layers, the following experiments are all based on the preparation of Example 1. The lower 1 / 3 and upper 2 / 3 of the vaginal mucosa are tested respectively:
[0077] Experiment 1: residual cell detection
[0078] The vaginal matrix material prepared in Example 1 of the present invention was stained with conventional hematoxy...
experiment example 2
[0124] AVML, AVMU and SIS were used as scaffold materials to construct a rat vaginal partial resection model. A rubber hose with a length of 1.8 cm and an outer diameter of 0.4 cm was used as a vaginal mold, and the transplantation test was divided into three groups: AVML stent group (AVML group), AVMU stent group (AVMU group) and SIS stent group (SIS group). Take 2 to 3 month old healthy SD rats, female, and inject 1% pentobarbital sodium 50mg / kg intraperitoneally, after successful anesthesia, fix the limbs in the supine position, expose the lower abdomen and vagina, prepare the skin, and disinfect with 75% alcohol skin, povidone iodine to disinfect the vagina. The three groups of materials are rolled on the mold and sewed with 4-0 silk thread into a blind tube shape. Cut the skin longitudinally about 1 cm above the pubic symphysis about 3 cm, separate the rectus abdominis, open the peritoneum, resect the uterine body and upper 1 / 2 of the vagina, anastomose the blind tubular...
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