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Vaginal substrate material and preparation method thereof

A matrix material and vaginal technology, applied in the field of vaginal matrix material and its preparation, can solve the problems of biological activity and biomechanical properties, and the treatment effect needs to be further improved, and achieve low immunogenicity and cytotoxicity, biocompatibility and biological The effect of good mechanical properties and good application selection

Active Publication Date: 2015-11-25
黄向华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, although the application of existing materials has played a certain therapeutic effect, its biological activity, biomechanical properties and therapeutic effect need to be further improved.

Method used

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  • Vaginal substrate material and preparation method thereof
  • Vaginal substrate material and preparation method thereof
  • Vaginal substrate material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Preparation of porcine vaginal matrix material:

[0054] (1) Take the vaginal tissues of healthy adult pigs (the warm ischemia time is less than 30 minutes), and wash the obtained vaginal tissues repeatedly with sterile saline. The knife and surgical scissors cut and separate the outer membrane layer, muscular layer and mucous layer of the vaginal tissue. When tissue folds are encountered, the manipulator is used to peel off. Repeatedly, the outer membrane layer and muscular layer of the vaginal tissue are stripped and removed. A vaginal mucosal layer with no damage and uniform thickness was collected. Then cut into appropriate size, such as width 7.56±1.42cm, length 11.63±1.84cm, thickness 0.067±0.014cm, rinse for later use.

[0055] (2) Shake the vaginal mucosa layer treated in step (1) with sterile deionized water at 4°C for 48 hours, and the shaking speed is 130rpm;

[0056] The sterile deionized water used contained 0.1 mM / L of protease inhibitor phenylmethylsulf...

experiment example 1

[0074] Experimental Example 1: Detection and evaluation of the biological properties of the materials prepared in Example 1 and Comparative Example 1:

[0075] In this experimental example, the biological performance of the vaginal matrix material prepared in Example 1 of the present invention was tested and evaluated through Test 1 to Test 6, and compared with the performance of the acellular small intestinal submucosa matrix prepared in Comparative Example 1.

[0076] Since the outer 1 / 3 of the vagina (that is, the lower 1 / 3 of the vagina) and the 2 / 3 of the vaginal inner segment (that is, the upper 2 / 3 of the vagina) are differentiated from different germ layers, the following experiments are all based on the preparation of Example 1. The lower 1 / 3 and upper 2 / 3 of the vaginal mucosa are tested respectively:

[0077] Experiment 1: residual cell detection

[0078] The vaginal matrix material prepared in Example 1 of the present invention was stained with conventional hematoxy...

experiment example 2

[0124] AVML, AVMU and SIS were used as scaffold materials to construct a rat vaginal partial resection model. A rubber hose with a length of 1.8 cm and an outer diameter of 0.4 cm was used as a vaginal mold, and the transplantation test was divided into three groups: AVML stent group (AVML group), AVMU stent group (AVMU group) and SIS stent group (SIS group). Take 2 to 3 month old healthy SD rats, female, and inject 1% pentobarbital sodium 50mg / kg intraperitoneally, after successful anesthesia, fix the limbs in the supine position, expose the lower abdomen and vagina, prepare the skin, and disinfect with 75% alcohol skin, povidone iodine to disinfect the vagina. The three groups of materials are rolled on the mold and sewed with 4-0 silk thread into a blind tube shape. Cut the skin longitudinally about 1 cm above the pubic symphysis about 3 cm, separate the rectus abdominis, open the peritoneum, resect the uterine body and upper 1 / 2 of the vagina, anastomose the blind tubular...

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Abstract

The invention discloses a vaginal substrate material and a preparation method thereof, wherein a vaginal tissue mucous layer of a pig or a cow is taken as a carrier, and is subjected to the following treatment: a, carrying out shocking treatment by adopting deionized water solution containing phenylmethylsulfonyl fluoride or Tris solution; b, carrying out shocking treatment in a water solution containing lauryl sodium sulfate or Tris-HCl buffer solution; c, carrying out shocking treatment by adopting Tris-HCl buffer solution containing Triton X-100; d, carrying out shocking treatment in Tris-HCl buffer solution containing deoxyribonuclease and ribonuclease; and e, carrying out disinfection and freeze-drying. The vaginal substrate material provided by the invention has the biological activity and the biological mechanical property more suitable for pelvic floor repairing or vagina reconstruction.

Description

technical field [0001] The invention relates to an extracellular matrix and a preparation method thereof, in particular to a vaginal matrix material and a preparation method thereof. Background technique [0002] Extracellular matrix material (also known as acellular matrix material) is a complex of structural and functional proteins remaining after decellularization of tissues or organs, which is composed of collagen, elastin, glycosaminoglycans and various growth factors. It plays an important role in cell adhesion, cell differentiation and connection between cells and biomaterials. Moreover, the extracellular matrix material will be gradually degraded into monopeptide substances after transplantation, which have strong biological activity, antibacterial properties, chemotaxis and mitogenic properties of host cells. Because of its wide source, non-immunogenicity, good biocompatibility, biological activity and antibacterial properties, it has attracted more and more attent...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 黄向华张敬坤张琳李亚楠张明乐张志强杜润璇
Owner 黄向华
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