3 results about "Inner segment" patented technology

The present invention belongs to the field of biotechnology, and discloses a capsid protein mutant that improves the infectivity of AAV to eye tissues and its application. By inserting AAVRFDGTERAA and RQYSDAVRAE at amino acid positions 587 and 588 corresponding to the wild-type AAV2 virus capsid protein, the capsid protein mutants AAV2-NE01 and AAV2-NE02 are obtained. The virus yields of AAV2-NE01 and AAV2-NE02 are 1.96 times and 1.20 times that of AAV2-WT. After intravitreal injection, they can preferably infect various layers of cells from the ganglion cell layer (GCL), inner nuclear layer (INL) to outer nuclear layer (ONL) of the NHP retina and the trabecular meshwork region, with a high infection positive rate, strong fluorescence intensity, strong retinal penetration ability, and can infect the nuclear part and inner segment of the optic nerve. It has good application prospects and is expected to be developed into an AAV vector for treating eye diseases.

Anti-extrusion elements to prevent extrusion of a sealing element during compression. The anti-extrusion element has a series of inner segments forming a loop bent to form a slot running along the circumference of the anti-extrusion element. The slot opens and faces radially outward away from the loop. Each inner segment has two ends on opposing sides aligned to form an opening between two inner segments. The anti-extrusion element additionally has a series of outer segments adjacent to one another to form a loop and slot. The series of inner segments is nested within the slot of the series of outer segments. Each outer segment comprises a peg within the slot and each peg aligns with a corresponding opening such that one peg protrudes through one opening. The anti-extrusion element additionally has a plurality of springs in the slot of inner segments and the springs are coupled to the pegs.

A type of under-eye implant includes two mirror-symmetrical implant units. Each implant unit is arc-shaped, tapering at both ends and thickening in the middle along its length. All edges and surfaces are smoothly transitioned through rounded surfaces. The arc trajectory of the implant unit matches the natural contour of the lower eyelid from the inner corner to the outer corner, with the radius of curvature of the inner segment near the inner corner being larger than that of the outer segment near the outer corner. Each implant unit has a through-hole at each end. The implant is made of expanded polytetrafluoroethylene (ePTFE), whose microporous structure induces tissue cell ingrowth after implantation, preventing implant displacement and light transmission, and demonstrating good biocompatibility.