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Method and system for extraction and separation of isovitexin from Desmodium styracifolium

A technology of Radix Polygoniflora and Isovitexin is applied in the field of phytochemistry and achieves the effects of simple process operation, high product yield and short production time

Active Publication Date: 2015-11-25
WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods and systems for extracting and separating isovitexin need to be improved urgently

Method used

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  • Method and system for extraction and separation of isovitexin from Desmodium styracifolium
  • Method and system for extraction and separation of isovitexin from Desmodium styracifolium
  • Method and system for extraction and separation of isovitexin from Desmodium styracifolium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Figure 5 Show the general flow process of the inventive method, comprise following:

[0033] 1. Raw material processing: select 45,000-50,000 parts by weight of the leguminous plant Desmodium glabra, cut into 5-10 cm sections, wash off the sediment with drinking water, drain, and feed.

[0034] 2. Ethanol extraction: 80% ethanol reflux extraction twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, recover the ethanol until it has no alcohol smell.

[0035] 3. Concentration under reduced pressure: add water to dilute the extract concentrate to 5 times the volume of the medicinal material; filter through a 200-mesh filter cloth; get the column liquid; pack the net product resin that has been treated with 95% ethanol in advance, and replace it with purified water; The upper column liquid is pumped into the column for adsorption, and the flow rate is controlled to be 0.5-1 times BV / h. ...

Embodiment 2

[0046] Select 45,000-50,000 parts by weight of the leguminous plant Desmodium glabrata, cut into 5-10cm sections, wash off the sediment with drinking water, drain and feed. Reflux extraction with 80% ethanol twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, recover the ethanol until it has no alcohol smell. Dilute the extract concentrate with water to 5 times the volume of the medicinal material; filter with a 200-mesh filter cloth; get the upper column liquid; pack the net resin that has been treated with 95% ethanol in advance, and replace it with purified water; pump the upper column liquid Put it into the column, absorb, control the flow rate of 0.5-1 times BV / h, after the adsorption is completed, let it stand for 2 hours; rinse with purified water, the elution flow rate is 3-4BV / h, and the elution volume is 10BV; then elute with 60% ethanol , the elution flow rate is 2-3BV / h, and the...

Embodiment 3

[0049] Select 45,000-50,000 parts by weight of the leguminous plant Desmodium glabrata, cut into 5-10cm sections, wash off the sediment with drinking water, drain and feed. Reflux extraction with 80% ethanol twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, recover the ethanol until it has no alcohol smell. Dilute the extract concentrate with water to 5 times the volume of the medicinal material; filter with a 200-mesh filter cloth; get the upper column liquid; pack the net resin that has been treated with 95% ethanol in advance, and replace it with purified water; pump the upper column liquid Put it into the column, absorb, control the flow rate of 0.5-1 times BV / h, after the adsorption is completed, let it stand for 2 hours; rinse with purified water, the elution flow rate is 3-4BV / h, and the elution volume is 10BV; then elute with 60% ethanol , the elution flow rate is 2-3BV / h, and the...

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Abstract

The invention provides a method for extraction and separation of isovitexin. The method comprises the following steps of subjecting Desmodium styracifolium to reflux extraction by utilizing ethyl alcohol so as to obtain a Desmodium styracifolium extract; and subjecting the Desmodium styracifolium extract to multistage chromatographic separation by utilizing an alcohol system, wherein the step of multistage chromatographic separation comprises the following sub-steps of subjecting the Desmodium styracifolium extract to a first chromatographic separation by utilizing a first alcohol system so as to obtain a first separation part, subjecting the first separation part to a second chromatographic separation by utilizing a second alcohol system so as to obtain a second separation part, and subjecting the second separation part to a third chromatographic separation by utilizing a third alcohol system so as to obtain the isovitexin. The invention also provides a system for implementing the above-mentioned method. By utilizing the method and / or the system for extraction and separation of the isovitexin from the Desmodium styracifolium, process operation is simple; production time is short; product yield is high; purity is high; and the isovitexin with a purity reaching to 99.7% can be obtained.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry. Specifically, the invention relates to a method and a system for extracting and separating isovitexin from Desmodium sativa. Background technique [0002] Desmodium styracifolium (Osbeck) Merr. is a plant of the genus Desmodium styracifolium (Osbeck) Merr. of the leguminous family. Its medicinal part is the dry aerial part, and its main chemical components are flavonoids, saponins, polysaccharides, alkaloids and other compounds. It has the effects of clearing away heat and dehumidification, diuresis and treating stranguria. It is used for hot shower, sand shower, stone shower, astringent and painful urination, edema and oliguria, jaundice, red urine, and urinary calculi. [0003] Isovitexin (isovitexin) is also known as isovitexin, or 6-C-glucoseparinin, which is yellow powder; molecular formula: C 21 h 22 o 10 ;Molecular weight: 432; figure 1 Show its chemical structure. [0004] Is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 王学海李莉娥许勇杨仲文余通冯芸杨婷尹海龙黄璐曹儒宾谢长
Owner WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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