A kind of preparation method of denitrification microbial bacterial agent

Abstract

The invention discloses a preparation method of a denitrification microbial bacterial agent, which belongs to the technical field of biology and water treatment. The specific method is: activate the aerobic denitrification microbial preparation and inoculate it in the film area formed by the double-layer filler. The double-layer filler can allow the passage of the culture medium and the metabolites produced by the microorganism, but the microorganism cannot pass through. Under a certain pressure, the filler on one side flows into the membrane area at a certain speed, which is used for the growth of the microorganism, and the metabolites produced by the microorganism are discharged from the filler on the other side, and the high-concentration culture of the microorganism is carried out; Centrifuge to prepare a highly concentrated bacterial suspension, add a protective agent, dry and spray evenly onto a sterilized carrier, and store in a sealed container. After the denitrification microbial bacterial agent prepared by the invention is stored for a long time, its ammonia nitrogen degradation performance and bacterium reproduction and activation performance are good, the bacterium has good growth potential, and the bacterium has a high removal rate of ammonia nitrogen.

Description

technical field [0001] The invention relates to the technical field of biology and water treatment, in particular to a preparation method of a denitrification microbial bacterial agent. Background technique [0002] Excessive accumulation of nitrogen in water bodies will cause eutrophication of water bodies and seriously endanger the safety of ecosystems. The treatment of nitrogenous wastewater is a major issue in environmental pollution control. There are mainly physical, chemical and biological methods for denitrification of water bodies. Compared with physical and chemical methods, biological methods have the advantages of low treatment cost and no secondary pollution, so biological denitrification is currently recognized as one of the most economical and effective methods in wastewater denitrification. [0003] On the one hand, biological denitrification requires a large amount of microbial agents, and it is necessary to expand the cultivation of denitrification microb...

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Problems solved by technology

[0004] On the other hand, the traditional theory holds that biological denitrification must be realized through two independent processes of nitrification and denitrification. Nitrification and denitrification cannot occur at the same time. However, the traditional two-stage biological denitrification process generally has the disadvantages of high infrastructure investment and operating costs, large energy consumption, and complex operating control conditions.

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