Application of THBS2(Thrombospondin-2) as rectal cancer serum marker and diagnostic kit

A diagnostic kit and marker technology, applied in the biological field, to achieve the effect of assisting early diagnosis of colorectal cancer, high specificity and sensitivity

Inactive Publication Date: 2015-11-25
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there is no clear large sample data to verify the correlation between its serum expression level and tumor occurrence and development. The present invention shows that the specificity of serum THBS2 level in diagnosing colorectal cancer reaches 87.2%, and the sensitivity reaches 65.0%.

Method used

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  • Application of THBS2(Thrombospondin-2) as rectal cancer serum marker and diagnostic kit
  • Application of THBS2(Thrombospondin-2) as rectal cancer serum marker and diagnostic kit
  • Application of THBS2(Thrombospondin-2) as rectal cancer serum marker and diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] 1. Collection and preparation of serum samples:

[0021] Collect whole blood samples of colorectal cancer patients and normal controls, centrifuge them at 3000g for 5 minutes within 2 hours at room temperature, and take the supernatant to obtain serum. The obtained serum can be used for testing immediately, or stored at -80°C after aliquoting.

[0022] 2. The configuration method of Elisa's various buffers and reagents

[0023] (1) Coating buffer: pH9.6, 0.05M carbonate buffer

[0024]

[0025] (2) Sample diluent: pH7.4, 0.01mol / L PBS buffer

[0026]

[0027] (3) Washing buffer: PH7.4, 0.01M PBST buffer (containing 0.05% Tween-20)

[0028]

[0029] (4) Blocking solution: pH7.4 PBS solution containing 0.5% BSA.

[0030]

[0031] (5) Substrate color developing solution:

[0032] Chromogenic Solution A

[0033]

[0034] Chromogenic fluid B: (now equipped for current use)

[0035]

[0036] (6) Stop buffer: 2MH 2 SO 4

[0037]

[0038] (7) The antibody (Anti-THBS2) was provided by R&D comp...

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Abstract

Application of THBS2(Thrombospondin-2) as a rectal cancer serum marker and a diagnostic kit are disclosed, and belong to the technical field of biology. On the one hand, the invention provides application of THBS2 as the rectal cancer serum marker, and on the other hand, the invention provides the colorectal cancer diagnostic kit. The beneficial effects comprise that the provided serum biological marker has the specificity of 87.2% and the sensitivity of 65.0%, and possesses relatively high specificity and sensitivity; and the technical scheme provides a sensitive reliable operable method for quantitative determination on human serum THBS2 antigen level, and is beneficial for auxiliary early-stage diagnosis on colorectal cancer.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of THBS2 as a serum marker for rectal cancer and a diagnostic kit. Background technique [0002] Colorectal cancer is one of the most common malignant tumors. The mortality rate of colorectal cancer ranks the top three in the world. The incidence of colorectal cancer in China is on the rise, and the mortality rate ranks fifth. After active surgical treatment and radiotherapy and chemotherapy, the 5-year overall survival rate of colorectal cancer patients is about 66%. Data shows that if colorectal patients are effectively diagnosed and treated in stage I or even earlier, the survival rate can be increased to more than 90%. However, the current early diagnosis rate of colorectal cancer is only about 37%. Once the treatment is found in stage III or IV, the survival rate is reduced to 10%-30%. It can be seen that improving the early diagnosis rate of colorectal c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577
CPCG01N33/57446G01N33/577
Inventor 胡晓彤何超费伟强
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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