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Wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 and application thereof

A kind of hair grape and genetic technology, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2015-12-02
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that the bZIP transcription factor family has many important biological functions, including the expression of seed storage genes, plant growth and development, light signal transduction, disease defense, abiotic stress response, and ABA sensitivity. response, but the functional research on the grape bZIP gene has not yet been published

Method used

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  • Wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 and application thereof
  • Wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 and application thereof
  • Wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Germination of overexpressed transgenic lines and wild controls under osmotic stress (specific data are shown in Table 1)

[0026] The three transgenic lines (L7, L27, L42) and the wild control (WT) began to germinate on MS medium at 2 days, and the germination rate could reach more than 93% after 5 days ( Figure 1a shown). After culturing on the medium containing sodium chloride and mannitol for 5 days, the germination rates of the transgenic lines (L7, L27, L42) on the sodium chloride medium reached 54.31%, 58.76%, and 60.32%, respectively, while The germination rate of the wild control (WT) was only 10.99%, significantly lower than that of the transgenic plants ( Figure 1b shown). On the medium containing mannitol, the germination rates of the transgenic lines (L7, L27, L42) reached 58.30%, 62.95%, 71.86%, respectively, while the germination rates of the wild control (WT) were only 17.92% ( Figure 1c Shown), the germination rate among the three is co...

Embodiment 2

[0029] Embodiment 2: The resistance of the T3 generation seedlings of the wild hair grape merchant-24 stress-resistant gene VqbZIP39 transgenic Arabidopsis to osmotic stress (specific data are shown in Table 2)

[0030] In order to further measure the response of VqbZIP39 transgenic lines (L7, L27, L42) to osmotic stress, the inventors transplanted 7-day-old seedlings sown in MS medium to MS medium containing 125mM NaCl And 200mM mannitol osmotic medium was treated with osmotic stress, and the chlorophyll content, electrical conductivity, malondialdehyde (MDA) content, proline content and endogenous ABA content were measured after one week of treatment. The results showed that the chlorophyll contents of the three transgenic lines (L7, L27, L42) were 0.28, 0.33, 0.33 mg / g fresh weight respectively after sodium chloride stress, while the chlorophyll contents of the wild control (WT) were 0.10 mg / g. gFresh weight. After mannitol treatment, the chlorophyll contents of the three ...

Embodiment 3

[0035] Example 3: The resistance of T3 generation plants of the wild hair grape merchant-24 stress-resistant gene VqbZIP39 transgenic Arabidopsis thaliana to salt stress and drought stress.

[0036] It has been proved in previous studies that the stress-resistant gene VqbZIP39 of the wild Vitis vinifera-24 responds positively to both salt stress and mannitol stress, so the inventors focused on the responses of adult Arabidopsis plants to various stresses.

[0037] In order to avoid the influence of external and human factors, the inventors planted transgenic plants [VqbZIP39-7(L7), VqbZIP39-27(L27) and VqbZIP39-42(L42)] and wild controls in the same plug (such as Figure 3a-a ). The three-week-old seedlings were treated with salt stress and drought stress. The results showed that after one week of salt stress treatment, the transgenic plants (L7, L27, L42) and the wild control (WT) showed significantly different traits. The wild control plants all appeared more serious yellow...

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Abstract

The invention provides a wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 and application thereof. A pCAMBIA2300-35S-VqbZIP39 overexpressing vector is established for the first time, and is guided into modular Arabidopsis thaliana through a trivia impregnation method, an overexpressing transgenic line of the wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 and growth states of a wild contrast under the condition of osmotic stress treatment are researched, physical signs relevant to stress are analyzed, and growth situations of the transgenic line and the wild contrast under conditions of long-term drought stress and salt stress treatment, and expression quantity of a downstream stress related gene are researched. An experiment result shows that by overexpressing of the wild vitis quinquangularis Shang-24 stress tolerance gene VqbZIP39 in the Arabidopsis thaliana, salt stress resistance and drought stress resistance of plants are improved.

Description

technical field [0001] The invention belongs to the technical field of plant stress-resistant gene identification and genetic engineering, and specifically relates to a wild Vitis vinifera-24 stress-resistant gene VqbZIP39 and its application. Background technique [0002] Basic leucine zipper bZIP transcription factor is the most widely distributed and conserved protein among eukaryotic transcription factors, and is found in humans, animals, plants, microorganisms and insects. At present, bZIP transcription factors have been discovered in various plants, for example, there are 75 in the Arabidopsis genome, 89 or 92 in the rice genome, 47 in soybean, 92 in sorghum, and 125 in maize. 47 or 55 were found in the grape genome and so on. According to the similarity and conservation of their structural domains, they can be divided into 10 subfamilies, namely A, B, C, D, E, F, G, H, I, S; or 13 subfamilies, namely A, B, C, D, E, F, G, H, I, J, K, L, S, etc. The structural charac...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/84A01H5/06
Inventor 王西平涂明星
Owner NORTHWEST A & F UNIV
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