Ageratina adenophora extract and mancozeb compound bactericide

Abstract

The invention discloses an ageratina adenophora extract and mancozeb compound bactericide. The bactericide has the active ingredients of mancozeb and agerophorone. The compound bactericide adopts the natural plant extract agerophorone and mancozeb for compounding, reduces the usage amount of a traditional pesticide mancozeb, and besides, increases the usage effect; the compound bactericide is a bactericide with high efficiency, low toxicity, fast degradation speed and convenient use, can effectively prevent and treat phytophthora capsici leonian, pythium myriotylum and other plant fungal diseases.

Description

technical field

[0001] The invention relates to a compound bactericide, in particular to a compound bactericide of Eupatorium adenophorum extract and mancozeb. Background technique

[0002] Eupatorium adenophorum is a perennial herb or semi-shrub. The rhizome is thick and well developed, erect, the plant height is 30-200 cm, the branches are opposite and oblique, the stem is purple, covered with white or rust-colored pubescence. It is a common weed growing in southern my country. The extracts extracted by different methods of Eupatorium adenophorum have different functions. Patent 200810058143.2 reports that the extract of Eupatorium adenophorum can be used as a rooting agent. Patent 201310007596.3 reports that the extract of Eupatorium adenophorum can kill roundworm eggs. Patent 02113396.4 reports Eupatorium adenophorum powder has anti-rheumatic effect. Patent 200810008066.X reports the application of Eupatorium adenophorum extract as an antifouling agent for antifouling ...

Examples

Embodiment 1

[0015] Example 1 Extraction of Eupatorium adenophorum Extract

[0016] The collected leaves of Eupatorium adenophorum were dried in the shade and pulverized into a coarse powder, adding methanol 6 times the weight of the coarse powder, ultrasonically extracting at room temperature three times, 12 hours each time, combining the filtrates, concentrating under reduced pressure at 45°C to obtain an extract. Add a small amount of methanol to the extract to dissolve, add distilled water to dilute the methanol content to 20%, extract with ethyl acetate, recover the solvent under reduced pressure at 45°C from the extract to dryness, and obtain the ethyl acetate extract. The ethyl acetate extract was dissolved in a mixed solution of methanol:water:chloroform (85:15:5), and eluted on an XAD-2 macroporous resin column to remove impurities such as pigments, and the eluent was collected and concentrated to obtain a paste. The paste after decolorization was subjected to repeated silica ge...

Embodiment 2

[0017] Embodiment 2 antibacterial experiment

[0018] For the test bacteria: Phytophthora capsici, Pythium ingross.

[0019] Test medicament: mancozeb (Qingdao Dongsheng Pharmaceutical Co., Ltd.), and zelenone were prepared by the method in Example 1.

[0020] The extract of Eupatorium adenophorum and the combined fungicide containing the extract of Eupatorium adenophorum were tested for in vitro biological activity of the tested pathogenic bacteria by the method of mycelial growth rate. Calculate the bacteriostatic rate by following formula: Bacterial colony growth diameter (mm)=two times measure diameter average value-4.0 (bacteria cake diameter); Inhibition growth rate=(the diameter of control bacterium colony-the diameter of processing bacterium colony) / the diameter *100 of control bacterium colony %.

[0021] According to the formula of Colby's method (1966) to calculate the control effect and compare with the measured control effect, it is simple and effective to eva...

Embodiment 3

[0031] Embodiment 3 is to the control field experiment of capsicum phytophthora

[0032] Prepare pharmaceutical composition 1, which contains 10 mg / L mancozeb, 250 mg / L zelenone, 50 mg / L xylene and 50 mg / L dimethyl sulfoxide, and the rest is water.

[0033] Prepare pharmaceutical composition 2, which contains 10 mg / L mancozeb, 250 mg / L zelenone, 50 mg / L xylene and 50 mg / L polyoxyethylene sorbitan fatty acid ester, and the rest is water.

[0034] Take 200m of pepper field for planting 2 , infected with Phytophthora capsici and developed disease, moderately infected, divided into two parts on average, applied the pesticides of medicament composition 1 and medicament composition 2, sprayed 1L each time, sprayed once every other day, sprayed 3 times, and after 15 days, applied the pesticide The pepper recovery rate of composition 1 was 90%, and the pepper recovery rate of agent composition 2 was 95%.

technical field

[0001] The invention relates to a compound bactericide, in particular to a compound bactericide of Eupatorium adenophorum extract and mancozeb. Background technique

[0002] Eupatorium adenophorum is a perennial herb or semi-shrub. The rhizome is thick and well developed, erect, the plant height is 30-200 cm, the branches are opposite and oblique, the stem is purple, covered with white or rust-colored pubescence. It is a common weed growing in southern my country. The extracts extracted by different methods of Eupatorium adenophorum have different functions. Patent 200810058143.2 reports that the extract of Eupatorium adenophorum can be used as a rooting agent. Patent 201310007596.3 reports that the extract of Eupatorium adenophorum can kill roundworm eggs. Patent 02113396.4 reports Eupatorium adenophorum powder has anti-rheumatic effect. Patent 200810008066.X reports the application of Eupatorium adenophorum extract as an antifouling agent for antifouling ...

Examples

Embodiment 1

[0015] Example 1 Extraction of Eupatorium adenophorum Extract

[0016] The collected leaves of Eupatorium adenophorum were dried in the shade and pulverized into a coarse powder, adding methanol 6 times the weight of the coarse powder, ultrasonically extracting at room temperature three times, 12 hours each time, combining the filtrates, concentrating under reduced pressure at 45°C to obtain an extract. Add a small amount of methanol to the extract to dissolve, add distilled water to dilute the methanol content to 20%, extract with ethyl acetate, recover the solvent under reduced pressure at 45°C from the extract to dryness, and obtain the ethyl acetate extract. The ethyl acetate extract was dissolved in a mixed solution of methanol:water:chloroform (85:15:5), and eluted on an XAD-2 macroporous resin column to remove impurities such as pigments, and the eluent was collected and concentrated to obtain a paste. The paste after decolorization was subjected to repeated silica ge...

Embodiment 2

[0017] Embodiment 2 antibacterial experiment

[0018] For the test bacteria: Phytophthora capsici, Pythium ingross.

[0019] Test medicament: mancozeb (Qingdao Dongsheng Pharmaceutical Co., Ltd.), and zelenone were prepared by the method in Example 1.

[0020] The extract of Eupatorium adenophorum and the combined fungicide containing the extract of Eupatorium adenophorum were tested for in vitro biological activity of the tested pathogenic bacteria by the method of mycelial growth rate. Calculate the bacteriostatic rate by following formula: Bacterial colony growth diameter (mm)=two times measure diameter average value-4.0 (bacteria cake diameter); Inhibition growth rate=(the diameter of control bacterium colony-the diameter of processing bacterium colony) / the diameter *100 of control bacterium colony %.

[0021] According to the formula of Colby's method (1966) to calculate the control effect and compare with the measured control effect, it is simple and effective to eva...

Embodiment 3

[0031] Embodiment 3 is to the control field experiment of capsicum phytophthora

[0032] Prepare pharmaceutical composition 1, which contains 10 mg / L mancozeb, 250 mg / L zelenone, 50 mg / L xylene and 50 mg / L dimethyl sulfoxide, and the rest is water.

[0033] Prepare pharmaceutical composition 2, which contains 10 mg / L mancozeb, 250 mg / L zelenone, 50 mg / L xylene and 50 mg / L polyoxyethylene sorbitan fatty acid ester, and the rest is water.

[0034] Take 200m of pepper field for planting 2 , infected with Phytophthora capsici and developed disease, moderately infected, divided into two parts on average, applied the pesticides of medicament composition 1 and medicament composition 2, sprayed 1L each time, sprayed once every other day, sprayed 3 times, and after 15 days, applied the pesticide The pepper recovery rate of composition 1 was 90%, and the pepper recovery rate of agent composition 2 was 95%.

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