Method and special primers and probes for DNA identification of Dalbergia chinensis

Abstract

The invention discloses a method, a special primer and a probe for identifying DNA (deoxyribonucleic acid) of siam rosewood payung among rosewood. The primer and the probe have special specificity for the siam rosewood payung. The method is a fluorescent quantitative PCR (polymerase chain reaction) method. The method, the special primer and the probe have the advantages that only a few detection samples are required, and destroy to wood and structural morphologies of finished products of the wood can be prevented; special DNA information of the wood is used as a basis, siam rosewood payung species identification results can be quickly, accurately and objectively acquired, accordingly, the shortcoming that the traditional identification methods need to be established on the basis of complete and easily identifiable morphological structures of detected wood can be overcome, and the method, the special primer and the probe have broad application prospects.

Description

technical field [0001] The invention relates to the field of molecular biology detection for wood species identification, in particular to a DNA identification method for Dalbergia cochinchinensis and special primers and probes. Background technique [0002] The "Redwood National Standard" was promulgated and implemented in 2000. In the "Redwood National Standard", the tree species within the "redwood" range are listed as "five genera and eight categories". The "five genera and eight categories" include a total of 33 species of wood. 5 genera: Pterostilbene, Dalbergia, Persimmon, Bean and Ironwood. Class 8: It is named after the trade name of the wood, namely rosewood, rosewood, fragrant wood, black rosewood, red rosewood, ebony, striped ebony and chicken wing wood. [0003] Cochin Dalbergia ( Dalbergia cochinchinensis ), commonly known as "Red Rosewood", belongs to the Dalbergia genus and Black Rosewood of the Fabaceae family in the national standard of mahogany. Produc...

AI Technical Summary

Problems solved by technology

However, neither the traditional recognition technology based on the experience of the recognizer nor the recognition technology based on computer image retrieval is separated from the morphological structure and characteristics of the wood itself, and the identification and recognition work must be based on the fact that the inspected wood has a complete and easily identifiable morphological structure. Basically, this brings certain limitations to the wood identification work. At the same time, similar species of wood tend to have similar material structures, which brings difficulties to the accuracy of wood identification.

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