Reversed-phase thin-layer chromatographic method for identifying florists chrysanthemum and bo chrysanthemum
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A technique of reversed-phase thin-layer chromatography and Gongju, applied in the field of identification of traditional Chinese medicinal materials, can solve the problems of inferior quality, mixed varieties, difficult identification methods, etc., and achieves the effects of good popularity and simple operation.
Active Publication Date: 2015-12-09
BEIJING INST FOR DRUG CONTROL
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Due to the different varieties and grades of commodities, the prices of various chrysanthemums sold in the market vary greatly, and because it is difficult to grasp the
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Embodiment 1
[0045] A method for identifying Gongju and Boju by reversed-phase thin-layer chromatography.
[0046] 1) Preparation of reference substance solution:
[0047] Accurately weigh luteolin, kaempferol, and acacetin reference substances respectively, place them in a 10ml measuring bottle, add 70% ethanol to dissolve and dilute to the mark, shake well, and make a reference substance solution containing 2.5mg per 1ml. spare;
[0048] 2) Preparation of the medicinal material test solution:
[0049] The dried Gongju and Boju medicinal materials are pulverized respectively with a high-speed universal pulverizer, and the medicinal material powder is obtained through a 60-mesh sieve;
[0050] Accurately weigh 0.25g of Gongju and Boju powders respectively, place them in a stoppered Erlenmeyer flask, add 25ml of 70% methanol, ultrasonicate for 30 minutes, filter, evaporate the filtrate to dryness on a water bath, add 1ml of methanol to dissolve the residue, Prepare the test solution of G...
Embodiment 2
[0063] A method for identifying Gongju and Boju by reversed-phase thin-layer chromatography.
[0064] 1) Preparation of reference substance solution:
[0065] Accurately weigh the reference substances of luteolin, kaempferol, and acacetin respectively, place them in a 10ml measuring bottle, add 80% ethanol to dissolve and dilute to the mark, shake well, and make a reference substance solution containing 3mg per 1ml, and set aside ;
[0066] 2) Preparation of the medicinal material test solution:
[0067] The dried Gongju and Boju medicinal materials are pulverized respectively with a high-speed universal pulverizer, and the medicinal material powder is obtained through a 60-mesh sieve;
[0068] Accurately weigh 0.3g of Gongju and Boju powder respectively, put them in a stoppered conical flask, add 25ml of 80% ethanol, ultrasonicate for 50 minutes, filter, evaporate the filtrate to dryness on a water bath, add 1ml of methanol alcohol to dissolve the residue , Prepare the tes...
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Abstract
The invention relates to a reversed-phase thin-layer chromatographic method for identifying florists chrysanthemum and bo chrysanthemum. The method particularly includes following steps: 1) employing luteolin, kaempferol and acacetin as reference substances and ethanol solutions of the florists chrysanthemum and the bo chrysanthemum as sample solutions, spotting the reference substances and the sample solutions on one reversed-phase thin-layer plate; 2) with methanol and water as developing solvents, developing the reference substances and sample solutions, air-drying the plate, performing development with an aluminum trichloride ethanol solution as a color developing agent; 3) after color development, performing inspection and identification under ultraviolet lights being 365 nm and 254 nm in wavelength, wherein in the position that the florists chrysanthemum is same as the luteolin reference substance, a weak fluorescent spot or dark spot is developed; and in the positions that the florists chrysanthemum is same as the kaempferol and acacetin reference substances, no fluorescent spot or dark spot is developed; in the position that the bo chrysanthemum is same as the luteolin reference substance, a strong fluorescent spot or a dark spot is developed; and in the position that the bo chrysanthemum is same as the kaempferol and acacetin reference substances, same fluorescent spot or dark spot is developed. In the invention, for the first time, the reversed-phase thin-layer chromatographic method is used for identifying the florists chrysanthemum and the bo chrysanthemum. The method is strong in specificity and can accurately identify the identifying florists chrysanthemum and the bo chrysanthemum.
Description
technical field [0001] The invention belongs to the technical field of identifying traditional Chinese medicinal materials, and relates to a reversed-phase thin-layer chromatography method for identifying chrysanthemums and chrysanthemums. Background technique [0002] Chrysanthemum is a commonly used traditional Chinese medicine and also a commonly used drink. The commercial chrysanthemum is the dry flower head of Chrysanthemum morifolium Ramat. Due to different origins and processing methods, chrysanthemum commercial medicinal materials are divided into Gongju, Boju, Hangju, etc., among which Gongju and Boju are both produced in Anhui Province and are common mainstream varieties. Due to the different varieties and grades, the prices of various chrysanthemums sold in the market vary greatly, and because the identification methods of the differences in appearance and properties of various chrysanthemums are difficult to grasp, the phenomenon of mixed varieties and shoddy on...
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