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High-accuracy prealbumin immunoturbidimetry detection kit

A technology for detection kits and prealbumin, applied in biological testing, material testing products, etc., can solve the problems of limited promotion, low accuracy, low sensitivity, etc., and achieve the effect of improving performance, reducing impact and enhancing stability

Inactive Publication Date: 2015-12-09
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the common prealbumin immune turbidimetry has the problems of low sensitivity and low accuracy, which limits the further promotion of this technology.

Method used

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  • High-accuracy prealbumin immunoturbidimetry detection kit
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  • High-accuracy prealbumin immunoturbidimetry detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A prealbumin immune turbidimetric detection kit, which includes reagent R1, reagent R2,

[0036] 1) The composition of the reagent R1 is:

[0037] Buffer 200mmol / L

[0038] Polyethylene glycol-600010mmol / L

[0039] Preservative 6mmol / L

[0040] EDTA 5mmol / L

[0041] Bovine serum albumin 5mmol / L

[0042] 2) The composition of the reagent R2 is:

[0043] Tris-Hcl buffer 200mmol / L

[0044] Preservative sodium azide 6mmol / L

[0045] EDTA 5mmol / L

[0046] Tween-201-5mmol / L

[0047] Bovine serum albumin 5mmol / L

[0048] Anti-human prealbumin antibody 30mmol / L

[0049] 3) The method of using the reagents in this example:

[0050] The prealbumin detection reagent described in this example is used in an automatic biochemical analyzer with dual reagent functions, such as Hitachi 7180 automatic analyzer, etc., and is determined by the endpoint method. Place R1 and R2 on the corresponding reagent positions according to the ratio of 3:1, and place distilled water, standa...

Embodiment 2

[0055] correlation experiment

[0056] The kit of Example 1 was used as the experimental group, and a prealbumin kit with excellent accuracy recognized on the market was used as the control group for comparative experiments, and 20 samples were detected. The detection results are shown in Table 2, and Correlation curves for the two reagents were obtained (eg figure 1 shown). The test results show that the two kits have a great correlation with the excellent kits in the market.

[0057] Table 2 Example 1 reagent and market common and recognized prealbumin assay kit comparative detection results

[0058] .

Embodiment 3

[0060] Reagent Stability Contrast Test

[0061] For the reagents in Example 1, 13 groups were evenly distributed, and the reagent volume of each group was 20mL for R1 and 5mL for R2; box for comparison. Place it in a refrigerator at 2-8°C, and take out a set of reagent test samples on the same day every month. Test results such as figure 2 As shown, the reagent of Example 1 is more stable than the common prealbumin assay kits in the market under the storage condition of 2-8°C.

[0062] In summary, the proalbumin immune nephelometry detection kit provided by the present invention can effectively improve the efficiency of the kit by adding a certain amount of bovine serum albumin and replacing the preservative with gentamicin in the reagent R2. Stability, accuracy of reagents. Therefore, the prealbumin immunoturbidimetric assay kit provided by the present invention is conducive to further popularization and use in the market.

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Abstract

The invention discloses a prealbumin immunoturbidimetry detection kit and belongs to the technical field of clinical in-vitro detection reagents. The kit comprises a first reagent R1 and a second reagent R2. The reagent R1 contains a Tris-Hcl buffer solution, polyethylene glycol-6000, the preservative gentamicin, ethylenediaminetetraacetic acid and bovine serum albumin. The reagent R2 contains a Tris-Hcl buffer solution, the preservative gentamicin, ethylenediaminetetraacetic acid, bovine serum albumin and a human prealbumin antibody. By adding the preservative gentamicin and the stabilizer bovine serum albumin to the reagent R1 and the reagent R2, the stability of the kit is effectively improved. The novel surface active agent tween-20 is adopted, so that measurement performance is improved remarkably and the stability and antijamming capability of the reagents are improved; the accuracy of the reagents is high, and further application and promotion in the market are facilitated.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a prealbumin detection reagent and a detection method using the detection reagent. Background technique [0002] Prealbumin, also known as transthyretin, is synthesized by liver cells. It is a tetramer composed of 4 identical subunits, with a molecular weight of 55.0kD, a sugar content of 0.5%, and a tryptophan content of 3.15%. Prealbumin has important biological activities. It cooperates with thyroxine-binding globulin and albumin to participate in the operation of thyroxine. It is second only to thyroxine-binding globulin (TBG) in the functioning of thyroid hormones. TBG is responsible for transporting 70%-75% of the total thyroid hormones, prealbumin transports 15%-20%, and albumin transports less. Under normal physiological conditions, almost all thyroxine is transported to target cells by combining with these three proteins. [0003] Anothe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 甘宜梧刘慧王春艳谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD