Hair restorer containing stem cells and preparation method thereof
A stem cell hair growth factor and stem cell technology, applied in the field of stem cell hair growth factor and its preparation, can solve the problems of protein type and quantity reduction, shortened lifespan, and decline in cell metabolism, so as to enhance hair softness and promote hair synthesis Activation, hair elasticity improvement effect
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Embodiment 1
[0050] Group A:
[0051]
[0052] Group B:
[0053]
[0054] Combine Group A and heat to 72 degrees. Heat deionized water to 72 degrees. Add group A into deionized water, mix evenly, stir and cool down.
[0055] When the temperature drops below 40 degrees, add group B and mix well.
[0056] The stem cell extract is sheep umbilical cord blood stem cells;
[0057] Wherein, the preparation method of the stem cell extract is as follows: collect the umbilical cord blood of sheep, add anticoagulant, keep the temperature at 4°C, dilute with normal saline, and the volume ratio of sheep umbilical cord blood and normal saline is 1:1;
[0058] Add the diluted sheep umbilical cord blood to the placenta active extract, the volume ratio of the diluted sheep cord blood to the placenta active extract is 1:1, then add a 6% gelatin solution in a mass ratio, the gelatin solution and placenta activity The volume ratio of the extract is 0.3:1. Gradient centrifugation is carried out to o...
Embodiment 2
[0063] Group A:
[0064]
[0065] Group B:
[0066]
[0067]
[0068] Mix group A and heat to 70-75 degrees. Heat deionized water to 70-75 degrees. Add group A into deionized water, mix evenly, stir and cool down.
[0069] When the temperature drops below 40 degrees, add group B and mix well.
[0070] Wherein, the preparation method of the stem cell extract is as follows: collect the umbilical cord blood of sheep, add anticoagulant, keep the temperature at 4°C, dilute with normal saline, and the volume ratio of sheep umbilical cord blood and normal saline is 1:1;
[0071] Add the diluted sheep umbilical cord blood to the placenta active extract, the volume ratio of the diluted sheep cord blood to the placenta active extract is 1.2:1, then add a 3% gelatin solution in a mass ratio, the gelatin solution and placenta activity The volume ratio of the extract is 0.5:1. Gradient centrifugation is carried out to obtain the cell suspension at the liquid phase interface; ...
Embodiment 3
[0076] Group A:
[0077]
[0078]
[0079] Group B:
[0080]
[0081] Mix group A and heat to 70-75 degrees. Heat deionized water to 70-75 degrees. Add group A into deionized water, mix evenly, stir and cool down.
[0082] When the temperature drops below 40 degrees, add group B and mix well.
[0083] Wherein, the preparation method of the stem cell extract is as follows: collect the umbilical cord blood of sheep, add anticoagulant, keep the temperature at 4°C, and dilute with normal saline, the volume ratio of sheep umbilical cord blood and normal saline is 1.2:1;
[0084] Add the diluted sheep umbilical cord blood to the placenta active extract, the volume ratio of the diluted sheep cord blood to the placenta active extract is 0.8:1, and then add a 5% gelatin solution in a mass ratio, the gelatin solution and placenta activity The volume ratio of the extract is 0.3:1. Gradient centrifugation is carried out to obtain the cell suspension at the liquid phase interf...
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