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Preparation method of glucan microsphere gel

A technology of glucan and spherogel, applied in the field of preparation of hydrogel microspheres, can solve the problems of excessive hydrolysis of PVAc, unfavorable industrial production, difficult process control, etc., and achieves stable properties, narrow particle size distribution, Controllable range of effects

Active Publication Date: 2015-12-16
南雄阳普医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is necessary to find a suitable alkaline point, which is beneficial to the cross-linking of dextran and cannot cause excessive hydrolysis of PVAc. There are too many influencing factors to be considered in this process, and the process is difficult to control, which is not conducive to industrial production.

Method used

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  • Preparation method of glucan microsphere gel
  • Preparation method of glucan microsphere gel
  • Preparation method of glucan microsphere gel

Examples

Experimental program
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Effect test

Embodiment 1

[0057] This embodiment includes a synthesis technique of cross-linked dextran microsphere gel with specific pore size, including the following steps:

[0058] 1) Prepare 2.5M sodium hydroxide solution: Weigh 10g of sodium hydroxide, add 100ml of distilled water, and stir until colorless and transparent. Weigh 6.0±0.5g into a clean container;

[0059] 2) Prepare the dispersed phase: Weigh 3.0±0.3g of dextran, add it to the 2.5M sodium hydroxide solution, and stir under the homogenizing emulsification equipment. The stirring parameter is 600±100rpm, 3-5min. The sugar is completely dissolved into a colorless and transparent solution, which is used as the dispersed phase for use.

[0060] 3) Weigh 60±5g castor oil and place it in a clean container for use.

[0061] 4) Preparation of continuous phase: Weigh out 802.5±0.3g of Span, add it to the castor oil mentioned above, stir under homogeneous emulsification equipment, the stirring parameter is 600±100rpm, 3-5min, at this time Span 80 is...

Embodiment 2

[0071] The dextran microsphere gel of this example is basically the same as that of example 1, except that the concentration of the dispersed phase is changed: in this example, the mass of dextran added in step 2) is 6.0±0.6 g.

[0072] Observe the morphology of the dextran microspheres prepared by the method of this embodiment with a biological microscope at 100 times, such as Figure 4 , Randomly select three microspheres for measurement, and get their radii respectively 39.62μm, 23.08μm, 48.40μm; take a sample to measure the particle size range of the gel microspheres, and the particle size distribution range is as follows Figure 5 ,, 40-120μm, can be applied to serological detection in the field of clinical diagnosis, such as microcolumn gel method to detect specific size infectious disease markers, cancer syndrome markers, etc., and for proteins, polysaccharides, and nucleic acids of different molecular weights , Separation and purification of enzymes and other substances.

Embodiment 3

[0074] The dextran microsphere gel of this example is basically the same as that of Example 1, and the difference is that the dispersing and emulsifying speed is changed: in this example, step 6) adjusts the homogeneous emulsification equipment parameter to 600±100rpm, 25- 30min.

[0075] Observe the morphology of the dextran microspheres prepared by the method of this embodiment with a biological microscope at 100 times, such as Image 6 , Randomly select three microspheres for measurement, and get their radii respectively 23.60μm, 33.94μm, 49.81μm. Sampling to measure the particle size range of the gel microspheres, the particle size distribution range is as follows Figure 7 , 40-120μm, can be used for serological testing in the field of clinical diagnosis, such as micro-column gel method to detect specific size infectious disease markers, cancer syndrome markers, etc., and used for proteins, polysaccharides, nucleic acids, Separation and purification of enzymes and other subst...

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Abstract

The invention discloses a preparation method of glucan microsphere gel. The method comprises the following steps: respectively preparing a dispersion phase glucan solution and a continuous phase solution; adding the dispersion solution to the continuous phase solution in a dropwise manner at a uniform speed, and fully emulsifying the obtained mixture by using a homogeneous emulsifying device; and adding a cross-linking agent, and mechanically stirring to fully cross-link the mixture in order to prepare the glucan microsphere gel. The glucan microsphere gel selects common domestic raw materials, has the advantages of low price, short supply period, simple process, large scale production, and no safety production or environment pollution hidden troubles, can be widely applied to different detection technologies, especially greatly promotes domestic popularization and application of micro-column gel detection technologies, and improves the medical level.

Description

Technical field [0001] The invention relates to a preparation method of hydrogel microspheres, in particular to a preparation method of cross-linked dextran microsphere gels with specific apertures. Background technique [0002] The microcolumn gel technology is an inspection technology for blood group serology invented by Frenchman Dr. YvesLappierre in 1986. It was certified by the US FDA in 1994 and has been used clinically as a routine technology in many developed countries. It belongs to size exclusion chromatography, and its main principle is to use different biological macromolecules with different hydrodynamic volumes, and achieve the purpose of separation through a gel with a certain pore size (gel void). Take the microcolumn gel method as an example. In the microcolumn tube, microsphere gel is injected as a filler. The gap between the microspheres and the microspheres forms a specific uniform pore size to form a gel molecular sieve. The red blood cells to be tested It b...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08L5/02
Inventor 刘强廖南山陈琼娣黄志强
Owner 南雄阳普医疗科技有限公司
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