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Genetic recombination brewer's yeast for degrading protein, construction method and application

A technology of Saccharomyces cerevisiae and gene recombination, which is applied in the field of bioengineering, can solve problems such as no related reports, and achieve the effect of improving degradation efficiency and low production cost

Active Publication Date: 2015-12-16
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no relevant reports at home and abroad on whether the expression of the sub gene after codon modification in the outer membrane of Saccharomyces cerevisiae can improve the enzyme activity

Method used

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  • Genetic recombination brewer's yeast for degrading protein, construction method and application
  • Genetic recombination brewer's yeast for degrading protein, construction method and application
  • Genetic recombination brewer's yeast for degrading protein, construction method and application

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Embodiment Construction

[0017] The present invention constructs a genetically engineered bacterium with high protease production, and studies its enzymatic characteristics. The engineered bacterium can be used for mass production of high-purity protease, has high expression, low cost, and wide application range, and provides a new method for the industrial production of protease , providing a technical reference for industrial mass production of the protease, and providing conditions for the development of industrial protease sources in oil and fat deep processing, bioenergy and other aspects.

[0018] The recombinant saccharomyces cerevisiae with protease displayed on the surface of the present invention expresses the protease on the cell surface, increases the contact area between the enzyme and the substrate, and achieves the purpose of improving the protein degradation efficiency. The protease gene was ligated into vector pYD1 and transformed into Saccharomyces cerevisiae EBY100. At the same time...

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Abstract

The invention discloses a genetic recombination brewer's yeast for degrading protein, and also discloses a construction method of the genetic recombination brewer's yeast. The genetic recombination brewer's yeast contains exogenous protease genes. Protease genes are subjected to codon reconstruction, are recombined with a brewer's yeast expression vector, and are transferred into brewer's yeast cells to obtain correct expression so as to construct the genetic recombination brewer's yeast. By the genetic recombination brewer's yeast, protein degrading efficiency of yeast cells is improved effectively. The genetic recombination brewer's yeast has features of low production cost, biomass reusability and zero secondary pollution, and plays an active driving role in degradation of protein in an environmental area, and large-scale fermentation and production of protein in an industry area.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically discloses a genetically recombined Saccharomyces cerevisiae capable of efficiently degrading proteins. [0002] The present invention also relates to the construction method of the above-mentioned gene recombinant Saccharomyces cerevisiae. [0003] The present invention also relates to the application of the above-mentioned gene recombinant Saccharomyces cerevisiae in degrading proteins. Background technique [0004] Microbial protease has the advantages of wide source and low cost, and plays an important role in industrial production. Serine protease is the most common type of protease, which is widely used in medicine, food, brewing and other industries, and has important research and application value. The current research mainly focuses on the screening of enzyme-producing strains, the study of enzymatic properties, and the cloning of enzyme-producing gene fragments....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12N15/81C12N15/57C12R1/865
Inventor 席北斗黄彩红何小松高如泰袁英赵昕宇党秋玲
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI