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A kind of bovine morganismi arthritis inactivated vaccine and preparation method thereof

A technology of Morganella bovis and Morganella bovis, which is applied in the field of inactivated vaccines for arthritis caused by Morganella bovis and its preparation, can solve the problems of chemical drug residues, water and environmental pollution, and achieve the goal of preparing The method is simple, the preparation process is simple, and the protective effect is good

Active Publication Date: 2018-09-21
福清市默克兽医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antibiotics and chemical drugs are mainly used to prevent and control M. bovis arthritis in clinical practice. However, long-term use of antibiotics is prone to drug resistance, and chemical drugs will cause residues, pollution of water bodies and the environment, and vaccines are used. Immunization can avoid these disadvantages

Method used

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  • A kind of bovine morganismi arthritis inactivated vaccine and preparation method thereof
  • A kind of bovine morganismi arthritis inactivated vaccine and preparation method thereof
  • A kind of bovine morganismi arthritis inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation and identification of Morganella morsii NL-1 (CCTCC NO: M2015583)

[0020] 1. Aseptically take the synovial fluid of the cow with arthritis, streak it and inoculate it on the normal nutrient agar medium, culture it at 37℃ for 24h, pick a single colony to obtain the pure culture, transfer it to the normal nutrient agar slant medium Preserved for animal regression test and identification. The typical colony on the ordinary nutrient agar plate is round, about 1.5~2.0mm in diameter, colorless or light yellow, translucent, indicating smooth, moist, neat edges, no water-soluble pigments, and the culture is accompanied by a special smell.

[0021] 2. Take the purely cultured bacteria on the above-mentioned slope to make a smear specimen, and examine the bacterial morphology by Gram staining microscope. Then inoculate the common nutrient medium to expand the culture to 10 9 CFU bacteria inoculate the knee joint cavity of two-month-old calves. Gram staining showed that t...

Embodiment 2

[0025] Preparation and safety test of inactivated vaccine of Morganella morsii NL-1 (CCTCC NO: M2015583)

[0026] 1. Preparation of inactivated vaccine of Morganella morsii NL-1 (CCTCC NO: M2015583)

[0027] The Morganella morganii NL-1 was streaked and inoculated on a common nutrient agar plate, and a single colony was picked and transferred to the fermentation medium for expansion culture as the seed solution. The seed solution is inoculated into the fermentation medium at a 0.1% (V / V) inoculum, and cultured at 37°C at 180 rpm for 24 hours, which is the cultured vaccine bacterial solution. Adjust the number of viable bacteria in the vaccine liquid to 10-50 billion CFU / mL, add formaldehyde with a final concentration of 0.5% in volume, and inactivate at 4°C for 72 hours. After the vaccine bacteria solution is inactivated, aluminum hydroxide adjuvant is added in a volume ratio of 1:4, and mixed to prepare an inactivated vaccine for Bovine Morganella morganii arthritis.

[0028] 2. S...

Embodiment 3

[0034] Evaluation of the immune effect of the Morganella morganii NL-1 (CCTCC NO: M2015583) inactivated vaccine

[0035] Randomly select 90 healthy Holstein bull calves of similar weight and divide them into three groups. Vaccine group: 30 calves were injected intramuscularly with 2 mL of the vaccine of the present invention in the neck triangle area at 30 days old, and 2 mL of the vaccine of the present invention was injected again at 60 days old; control group: 30 calves were injected into the neck triangle area muscle at 30 days old Inject 2 mL of sterilized normal saline, and again at 60 days of age, inject 2 mL of sterilized normal saline; normal control group: 30 calves as normal control group, no injection. The experimental animals were observed for 6 months. During the observation period, all experimental animals used the same feeding and management methods. Calculate the morbidity and mortality of experimental animals caused by Morganella morganii throughout the observa...

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Abstract

The invention discloses a bovine Morganella morganii arthritis inactivated vaccine and a preparation method thereof. Through pathogenic isolation and 16S rRNA sequencing, a pathogen is determined to be Morganella morganii, which is named as Morganella morganii NL-1 strain with a preservation number of CCTCC NO: M2015583. The inactivated vaccine is obtained by inoculating the bacterium into culture medium for amplification culture, harvesting a culture, inactivating the culture, then adding aluminum hydroxide adjuvant according to a ratio of 4:1 (V / V) and performing mixing. The immune effect of the Morganella morganii inactivated vaccine disclosed by the invention is sure, a protection duration of at least 5 months can be obtained by bovine immunizing, and a good effect of preventing and controlling bovine Morganella morganii arthritis can be achieved.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to an inactivated vaccine for Bovine Morganella morganii arthritis and its preparation method and application. Background technique [0002] Morganella morganii belongs to the genus Morganella in the Enterobacteriaceae and was first discovered by Morgan in 1906. The bacterium is a kind of rot parasitic bacteria, which is widely distributed in nature and often exists in the intestines of humans and animals. It is a conditional pathogen that causes serious infections. According to reports, Morganella morganii is pathogenic to many animals, such as nutria, turtles, snakes, koi, kangaroos, turtles, pigs and chickens. The infection of the bacteria can cause skin and muscle ulcers, abscesses and bleeding of various internal organs. In addition, Morganella morganii is the pathogenic bacteria of secondary hospital infections, causing pneumonia, bacteremia, urinary tract infection, gastr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
Inventor 张德明林燕花王锦祥李金凤车勇良张梦青
Owner 福清市默克兽医院
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