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Bovine Morganella morganii arthritis inactivated vaccine and preparation method thereof

A technology of Morganella bovis and Morganella bovis, which is applied in the field of inactivated vaccines for arthritis caused by Morganella bovis and its preparation, can solve the problems of water body and environmental pollution, chemical drug residues, etc., and achieve the goal of preparing The effect of simple process, simple preparation method, and low requirements for vaccine storage and transportation conditions

Active Publication Date: 2015-12-23
福清市默克兽医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antibiotics and chemical drugs are mainly used to prevent and control M. bovis arthritis in clinical practice. However, long-term use of antibiotics is prone to drug resistance, and chemical drugs will cause residues, pollution of water bodies and the environment, and vaccines are used. Immunization can avoid these disadvantages

Method used

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  • Bovine Morganella morganii arthritis inactivated vaccine and preparation method thereof
  • Bovine Morganella morganii arthritis inactivated vaccine and preparation method thereof
  • Bovine Morganella morganii arthritis inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation and Characterization of Morganella morganii NL-1 (CCTCCNO: M2015583)

[0020] 1. Aseptically take the joint fluid of arthritis-stricken cattle, inoculate it on the common nutrient agar medium by streaking, incubate at 37°C for 24 hours, pick a single colony to obtain a pure culture, and transfer it to the normal nutrient agar slant medium Save for animal regression test and identification. Typical colonies on ordinary nutrient agar plates are round, with a diameter of about 1.5-2.0 mm, colorless or light yellow, translucent, smooth, moist, with neat edges, no water-soluble pigment, and the culture has a special odor.

[0021] 2. Take the pure cultured bacteria on the above-mentioned slant, make a smear specimen, and check the bacterial morphology by Gram staining microscope. Then inoculated in ordinary nutrient medium to expand culture, with 10 9 CFU bacteria were inoculated into the knee joint cavity of two-month-old calves. Gram staining showed that the b...

Embodiment 2

[0025] Preparation and safety testing of Morganella morganii NL-1 (CCTCCNO: M2015583) inactivated vaccine

[0026] 1. Preparation of Morganella morganii NL-1 (CCTCCNO: M2015583) inactivated vaccine

[0027] Streak inoculation of Morganella morganii NL-1 on a common nutrient agar plate, pick a single colony and transfer it to the fermentation medium for expansion culture, as the seed solution. The seed liquid is inoculated into the fermentation medium according to the inoculum amount of 0.1% (V / V), and cultivated at 180 rpm at 37°C for 24 hours, which is the cultured vaccine bacterial liquid. Adjust the number of viable bacteria in the vaccine liquid to 10-50 billion CFU / mL, add formaldehyde with a final volume concentration of 0.5%, and inactivate at 4°C for 72 hours. After the vaccine bacteria liquid is inactivated, aluminum hydroxide adjuvant is added in a volume ratio of 1:4, and mixed to prepare an inactivated vaccine for Morganella bovis arthritis.

[0028] 2. Safety te...

Embodiment 3

[0034] Evaluation of immune effect of Morganella morganii NL-1 (CCTCCNO: M2015583) inactivated vaccine

[0035] A total of 90 healthy Holstein bull calves with similar body weight were randomly selected and divided into three groups. Vaccine group: 30 calves were intramuscularly injected with 2 mL of the vaccine of the present invention at the age of 30 days in the neck triangle, and 2 mL of the vaccine of the present invention was injected again at the age of 60 days; Inject 2 mL of sterilized normal saline, and inject 2 mL of sterilized normal saline again at the age of 60 days; normal control group: 30 calves were used as the normal control group without injection. The experimental animals were observed for 6 months, and all experimental animals adopted the same feeding and management method during the observation period. Calculate the morbidity and mortality rate of the experimental animals caused by Morganella morganii during the entire observation period; calculate the ...

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Abstract

The invention discloses a bovine Morganella morganii arthritis inactivated vaccine and a preparation method thereof. Through pathogenic isolation and 16S rRNA sequencing, a pathogen is determined to be Morganella morganii, which is named as Morganella morganii NL-1 strain with a preservation number of CCTCC NO: M2015583. The inactivated vaccine is obtained by inoculating the bacterium into culture medium for amplification culture, harvesting a culture, inactivating the culture, then adding aluminum hydroxide adjuvant according to a ratio of 4:1 (V / V) and performing mixing. The immune effect of the Morganella morganii inactivated vaccine disclosed by the invention is sure, a protection duration of at least 5 months can be obtained by bovine immunizing, and a good effect of preventing and controlling bovine Morganella morganii arthritis can be achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to an inactivated vaccine for arthritis caused by Morganella morganii bovis and a preparation method and application thereof. Background technique [0002] Morganella morganii belongs to the Enterobacteriaceae family and was first discovered by Morgan in 1906. The bacterium is a saprophytic bacterium that is widely distributed in nature and often exists in the intestinal tract of humans and animals, and is an opportunistic pathogen that causes severe infections. According to reports, Morganella morganii is pathogenic to a variety of animals, such as nutria, soft-shelled turtles, snakes, koi, kangaroos, turtles, pigs and chickens. The infection of this bacterium can cause skin and muscle ulceration, abscess and hemorrhage of various internal organs. In addition, Morganella morganii is also a pathogenic bacterium for secondary infection in hospitals, causing pneumonia, bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
Inventor 张德明林燕花王锦祥李金凤车勇良张梦青
Owner 福清市默克兽医院
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