Plasma marker, primer pair and kit for diagnosis of vascular calcification and application

A technology of vascular calcification and diagnostic reagents, applied in the field of biomedicine

Active Publication Date: 2015-12-23
THE FIRST AFFILIATED HOSPITAL HENGYANG MEDICAL SCHOOL UNIV OF SOUTH CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of plasma miR-32-5p as a marker of vascular calcification. We first found that miR32-5p is highly expressed in plasma of vascular calcification, which can be used as a clinical basis for the development of the kit

Method used

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  • Plasma marker, primer pair and kit for diagnosis of vascular calcification and application
  • Plasma marker, primer pair and kit for diagnosis of vascular calcification and application
  • Plasma marker, primer pair and kit for diagnosis of vascular calcification and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Expression levels of plasma miR-32-5p in vascular calcification patients and non-calcification control groups

[0020] 1. Research object

[0021] Sixty-six patients who underwent coronary angiography and dual-source CTC examination between June 2013 and May 2014 in the First Affiliated Hospital of Nanhua University were selected. Those with a history of myocardial infarction, history of coronary or peripheral arterial revascularization, hepatic and renal insufficiency, thyroid or adrenal dysfunction, abnormal calcium metabolism, chronic infection (active tuberculosis, COPD protracted stage), connective tissue disease, and tumor were excluded. , end-stage of immune system disease or mental illness and other diseases, etc. According to the Agatston method score (CACS) of coronary artery CTA, they were divided into coronary artery calcification group and control group, in which 33 patients with coronary artery calcification score equal to 0 points were in the ...

Embodiment 2

[0058] Example 2: Evaluation of the diagnostic effect of miR-32-5p on patients with vascular calcification

[0059] ROC curve analysis showed that miR-32-5p as a biomarker had a high diagnostic value for vascular calcification (AUC was 0.831). The detailed analysis results are shown in Table 2.

[0060] Table 2: ROC analysis results

[0061]

Embodiment 3

[0062] Embodiment 3: Vascular calcification diagnostic kit

[0063] The composition of the vascular calcification diagnostic kit for detecting plasma miR-32-5p content is shown in Table 3:

[0064] Table 3 Composition of Vascular Calcification Diagnostic Kit

[0065]

[0066] In Table 3, the PCR forward primer sequence of miR-32-5p in the kit for vascular calcification diagnosis is shown in SEQ ID NO.2, and the PCR forward primer sequence of miR-16 is shown in SEQ ID NO.5; corresponding PCR technology Common reagents required, such as: reverse transcriptase, reverse transcription buffer, SYBR premix, ROX dye, RNase-Free double distilled water, etc., are well known to those skilled in the art; in addition, it can also contain standards and controls Taste.

[0067]

[0068]

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Abstract

The invention discloses a plasma marker, a primer pair and a kit for a diagnosis of vascular calcification and an application. The invention discloses a plasma miRNA marker miR-32-5p used for the diagnosis of the vascular calcification. The marker and primers thereof can be used for preparing a diagnostic kit which is used for the clinical diagnosis, prevention and detection of the vascular calcification.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a plasma marker, primer pair, kit and application for vascular calcification diagnosis. Background technique [0002] Vascular calcification is a common pathological change in diseases such as diabetes, hypertension, atherosclerotic vascular disease, and other vascular injuries. It is one of the important factors for the high morbidity and mortality of cardiovascular and cerebrovascular diseases. considered to be an accurate predictor of cardiovascular adverse events. In the past, vascular calcification was considered to be the passive deposition of calcium salts in cells and extracellular matrix. In recent years, however, vascular calcification has been found to be an active, highly tunable biological process similar to bone formation. As the main constituent cells of the cardiovascular system, vascular smooth muscle cells change from a contractile phenotype to an osteob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/178
Inventor 刘江华祖旭宇
Owner THE FIRST AFFILIATED HOSPITAL HENGYANG MEDICAL SCHOOL UNIV OF SOUTH CHINA
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