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Novel print recognition based metronidazole electrochemical sensor, preparation method and application

A metronidazole and electrochemical technology, which is applied in the field of clinical analysis and food safety monitoring, can solve the problems of reducing the selectivity of conductive MIP sensors, etc., and achieve the effects of enhanced imprint recognition ability, wide applicability, and direct and efficient signal conversion

Inactive Publication Date: 2015-12-23
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the template is electroactive, it may undergo an electrochemical reaction to change its structure; on the other hand, although the conductive MIP can realize direct conduction between the sensitive membrane and the electrode, if the template or probe molecule is not recognized by imprinting, Reacting directly on the surface of the conductive film will reduce the selectivity of the conductive MIP sensor

Method used

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  • Novel print recognition based metronidazole electrochemical sensor, preparation method and application
  • Novel print recognition based metronidazole electrochemical sensor, preparation method and application
  • Novel print recognition based metronidazole electrochemical sensor, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: as figure 1 Shown, a kind of metronidazole electrochemical sensor that is used for measuring the metronidazole content in the metronidazole tablet, based on the novel imprint recognition, it adopts the following method to make:

[0055] 1. Preparation of carbon paste electrodes:

[0056] Mix graphite powder and paraffin oil in a mortar with a weight ratio of 84:16 to form a carbon paste, and then fill it tightly into a 10cm-long polypropylene plastic tube with an inner diameter of 2.5mm, and insert one end of the tube into a 1mm-diameter The copper wire is used as the connection point of the external circuit, and the carbon paste on the other end surface is polished into a smooth mirror surface with weighing paper to obtain a carbon paste electrode;

[0057] 2. Preparation of polyaniline-type conductive film carbon paste electrodes containing alkoxy silicon groups on the surface:

[0058] Place the freshly prepared carbon paste electrode in a solution co...

Embodiment 2

[0063] Embodiment 2: A kind of metronidazole electrochemical sensor based on the novel blot recognition for monitoring the metronidazole content in human plasma is made by the following method:

[0064] 1. Preparation of carbon paste electrodes:

[0065] Graphite powder and paraffin oil were thoroughly mixed in a mortar with a weight ratio of 74:26 to form a carbon paste, which was then tightly filled into a 10 cm long polypropylene plastic tube with a straight inner diameter of 2.5 mm. One end of the tube is inserted into a silver wire with a diameter of 1mm as the connection point of the external circuit, and the carbon paste on the other end is polished into a smooth mirror surface with weighing paper to obtain a carbon paste electrode;

[0066] 2. Preparation of polyaniline-type conductive film carbon paste electrodes containing alkoxy silicon groups on the surface:

[0067] Place the freshly prepared carbon paste electrode in a solution containing 0.08 molL -1 0.15molL ...

Embodiment 3

[0072] Embodiment 3: a kind of metronidazole electrochemical sensor based on the novel blot recognition for monitoring the content of metronidazole in human urine, it is made by the following method:

[0073] 1. Preparation of carbon paste electrodes:

[0074] Mix graphite powder and paraffin oil in a mortar with a weight ratio of 94:6 to form a carbon paste, and then fill it tightly into a 10cm-long polypropylene plastic tube with an inner diameter of 2.5mm, and insert one end of the tube into a 1mm-diameter The copper wire is used as the connection point of the external circuit, and the carbon paste on the other end is polished into a smooth mirror surface with weighing paper;

[0075] 2. Preparation of polyaniline-type conductive film carbon paste electrodes containing alkoxy silicon groups on the surface:

[0076] Place the freshly prepared carbon paste electrode in a solution containing 0.08 molL -1 0.18molL of AMTEOS -1 In sulfuric acid solution, the potential cycle s...

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Abstract

The invention provides a novel print recognition based metronidazole electrochemical sensor, a preparation method and an application. The preparation method of the sensor comprises following steps: graphite powder and paraffin oil are uniformly mixed to form carbon paste, a carbon paste electrode is prepared and put in a sulfuric acid solution for potential scan round, and a conductive film carbon paste electrode is prepared; sol proportionally prepared from APTMS (aminopropyl trimethoxysilane), TEOS (tetraethoxysilane), 2-ethoxyethanol, a metronidazole containing 2-ethoxyethanol solution, an NaOH solution and deionized water is dispensed on the surface of the conductive film carbon paste electrode dropwise, a heterogeneous double-layered-based molecularly imprinted polymer film modified carbon paste electrode is obtained and connected with an electrochemical device, and the sensor is assembled. The sensor has enhanced print recognition capacity and wide applicability, has the characteristics of high sensitivity, good selectivity, wide linear range, high precision and accuracy, low detection cost, environment-friendliness, portability, suitability for field monitoring and the like, further, the preparation process is simple, the cost is low, and the service life is long.

Description

technical field [0001] The invention belongs to the field of clinical analysis and food safety monitoring, and particularly relates to a metronidazole electrochemical sensor based on a novel imprint recognition, that is, a double-layer heterogeneous molecularly imprinted polymer, a preparation method and its application in medicine, clinical, food and related products ( Such as feed, etc.) in the application of nitroimidazole drug analysis and monitoring. Background technique [0002] Metronidazole belongs to 5-nitroimidazole drugs (wherein the English name and structure see figure 1 ), has antibacterial and antiprotozoal activity, and is often used in the treatment of human trichomoniasis, piriformis, amebic liver abscess and other diseases. Studies have shown that excessive use or abuse of metronidazole will cause it to accumulate in the human body, and when it reaches or exceeds a certain amount, it will induce epilepsy, peripheral neuropathy and disorders and other dise...

Claims

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Application Information

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IPC IPC(8): G01N27/48
Inventor 邓健肖妮李辉梁俊程健琳琚赛琴
Owner NANHUA UNIV
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