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How to identify Zihe cars

A technology of zihe and samples, which is applied in the field of identifying zihe, can solve the problems of large influencing factors, complicated experimental operation, and long analysis time, and achieve the effects of avoiding interference from human factors, simple experimental operation, and broad application prospects

Active Publication Date: 2017-05-31
成都裕坚医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The second way is to use thin-layer chromatography to identify, but this method has the following disadvantages: the experimental operation is complicated, the toxicity of the developer is relatively high, the artificial influence factors are large, the reproducibility is poor, and the analysis time is long due to the need for drying and color development

Method used

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  • How to identify Zihe cars
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  • How to identify Zihe cars

Examples

Experimental program
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preparation example Construction

[0029] Preparation of Reference Substance Solution Take Zihe, extract with organic solvent to obtain Zihe extract, absorb the Zihe extract, spot it on the silica gel G thin-layer plate, use toluene-acetone=3:2V / V As a developing agent, take it out, dry it, cover it with a glass plate, and leave a width for color development; after color development, scrape the glass plate at the corresponding position of the blue stripe spot in the color development area to cover the undeveloped part, dissolve it In absolute ethanol, ultrasonically extract, filter, centrifuge, and evaporate the filtrate to obtain the reference substance; the reference substance is dissolved in mobile phase to obtain the reference substance solution;

[0030] Preparation of the test solution: take the test sample, extract it with an organic solvent, obtain the test sample extract, add mobile phase to dissolve, and prepare the test solution;

[0031] Determination method Precisely draw 10 μl each of the referenc...

Embodiment 1

[0040] Example 1 Using the method of the present invention to identify Zihe car

[0041] Preparation of the reference substance: according to the method of Test Example 1, the extract of Zihecaria was prepared, the extract of Zihecaria was absorbed, and strips were spotted on the silica gel G thin-layer plate. : 2) as developing agent. Take it out, dry it, and cover it with a glass plate, leaving a width of about 1cm for color development. After the color develops, scratch the glass plate to cover the undeveloped part at the corresponding position of the blue stripe spot in the color developed area. Dissolve it in absolute ethanol, ultrasonically extract for 10 minutes, filter, centrifuge (4000r / h) for 10 minutes, and evaporate the filtrate to obtain the reference substance.

[0042] Mass spectrometry analysis of the reference substance Mass spectrometry conditions: ionization method: ESI, ionization voltage: 4.5 kV, ion source temperature: 250°C, separator temperature: 260°...

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Abstract

The invention relates to a method for identifying a human placenta, and belongs to the technical field of identification of Chinese medicine. The method capable of identifying the human placenta quickly and accurately is provided. The method for identifying the human placenta comprises the following steps that VD determination is conducted according to Chinese Pharmacopoeia (second part, 2010 edition) through the high-efficiency liquid chromatography; a reference substance solution and a test substance solution are each precisely sucked by 10 microliters to be injected to a liquid chromatograph for determination; if a test substance and a reference substance have the same characteristic peak, a sample to be tested is the human placenta, and conversely the sample to be tested is a counterfeit human placenta medicinal material.

Description

technical field [0001] The invention relates to a method for identifying Ziheche, belonging to the technical field of traditional Chinese medicine identification. Background technique [0002] Ziheche is a precious nourishing medicine processed from human placenta. Traditional Chinese medicine believes that the placenta is sweet, salty, and warm in nature, and enters the lung, heart, and kidney channels. Yuan Zhigong. Modern medical research believes that the placenta contains protein, sugar, calcium, vitamins, immune factors, female hormones, progesterone, steroid hormones, gonadotropins, adrenocorticotropic hormones, etc., which can promote the development of breasts, uterus, vagina, and testicles. It also has a stimulating effect on the thyroid gland, and has good effects on tuberculosis, bronchial asthma, anemia, etc. It can be ground into powder orally or enema can prevent measles or relieve symptoms. It also has a certain curative effect on portal cirrhosis ascites ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 沈芃曹晓红卢福李亚慧
Owner 成都裕坚医药科技有限公司