A kind of assay method of soil laccase activity
A determination method and activity determination technology are applied in the field of standardization determination of forest soil laccase activity, which can solve the problem that the determination method of laccase has no standardized process and the like.
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Embodiment 1
[0020] 1. Weigh 2 g (dry weight) of fresh soil (<2 mm), dissolve it in 20 mL of sterilized 0.9% NaCl (accurate volume). 30ºC, 170rpm, 1 hour. Filter with filter paper, take half of the filtrate, boil in boiling water for 30 minutes, negative control. All filtrates were stored at 4 ºC.
[0021] 2. Add 200μl reaction system to the 96-well plate in turn: 100 μL pH5.0 citrate-phosphate buffer; 50 μL soil extract; 50 μL of 80 mM ABTS (working concentration 20 mM ABTS).
[0022] 3. 96-well plate sample arrangement and description:
[0023] 96-well plate sample arrangement
[0024]
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Embodiment 2
[0032] 1. Weigh 5 g (dry weight) of fresh soil (<2 mm), dissolve it in 50 mL of sterilized 0.9% NaCl (accurate volume). 30ºC, 170rpm, 1 hour. Filter with filter paper, take half of the filtrate, boil in boiling water for 45 minutes, negative control. All filtrates were stored at 4 ºC.
[0033] 2. Add 200μl reaction system to the 96-well plate in turn: 100 μL pH5.0 citrate-phosphate buffer; 50 μL soil extract; 50 μL of 120 mM ABTS (working concentration 30 mM ABTS).
[0034] 3. 96-well plate sample arrangement and description:
[0035] 96-well plate sample arrangement
[0036]
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PUM
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