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Efficient hybridization method for rheum tanguticum

A hybridization method and rhubarb technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as difficulties in hybrid breeding and difficult to configure hybrid combinations.

Inactive Publication Date: 2016-01-06
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flowering period of Tangut Rhubarb is short, the flowering period of a single plant is 6-8 days, and the flowering period of a group is 20-25 days. Due to the short flowering period, it is difficult to configure enough hybrid combinations in a short period of time, making hybrid breeding very difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] On May 26-28, 2011, the Tanggu special rhubarb hybrid breeding was carried out in the Demonstration Garden of Chinese Medicinal Materials in Shouyang Town, Longxi County. The present invention was implemented according to the following steps:

[0036] 1. Use sulfuric acid paper to sew a paper bag with a width of 6cm and a length of 20cm

[0037] 2. Optimizing parents and configuring hybrid combinations

[0038] Select sturdy red petiole and stem plants as the male parent, select 20 green petiole and stem plants as the female parent, select the flower branch with a height of 140-160cm in the middle of the female parent as the hybrid flower spike, and remove the flowers from the lower part of the flower spike. Leave 15 well-developed flowers in the middle and cut off the upper spikes;

[0039] 3. Crossing at the beginning of flowering

[0040] 4. Collect mature fresh male plant anthers and put them into small bottles with lids for later use

[0041] Remove the freshly dehisced anth...

Embodiment 2

[0054] On May 27-30, 2013, the Tanggu special rhubarb hybrid breeding was carried out in Zakulu Town, Zhuoni County. The present invention is implemented according to the following steps:

[0055] 1. Use sulfuric acid paper to sew a paper bag with a width of 6cm and a length of 25cm;

[0056] 2. Select sturdy, high-yielding and high-quality plants as parents, use marked traits such as red petioles and stalk plants as male parents, select 10 green petioles and stalk plants as female parents, and select the middle height of the female parent's stalks 130-160cm The flower branches are used as hybrid flower spikes. The lower part of the flower spike is removed, leaving 20 well-developed flowers in the middle part, and the upper flower spike is cut off;

[0057] 3. Cross-breeding at the beginning of flowering 6 hours after a single plant blooms;

[0058] 4. Remove the freshly split anthers from the red petioles and stalks of the first flowers;

[0059] 5. Use nail scissors to cut off the 0...

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Abstract

The invention relates to an efficient hybridization method for rheum tanguticum. The method includes the steps that a, parchment paper bags which are 6 cm wide and 15-25 cm long are sewn; b, parents are selected preferably, and a hybridization combination is configured, wherein male parent and female parent plants are selected preferably, and female parent spicas and buds are reserved selectively; c, hybridization time is determined; d, anther of the mature and fresh male parent plants is collected for use; e, female parent buds are screened, and bud operation is carried out, wherein female parent buds are handpicked, and then bud operation is carried out; f, pollination and hybridization are performed, wherein pollen is shaken off, and the anther is inserted; g, bagging is carried out; h, bags are removed in time; i, hybrid seeds are harvested at the right moment; j, true-false authentication is carried out. Rheum tanguticum flower hybridization operation can be efficiently carried out in quantity to obtain the hybrid seeds, excellent variation groups are created, and the effect is remarkable in seed breeding.

Description

technical field [0001] The invention relates to medicinal plant hybrid breeding technology, in particular to a high-efficiency hybridization method of rhubarb tangut. Background technique [0002] Tangut rhubarb ( R. palmatum var. tanguticum Maxim. Ex Balf. ) is used as medicine with dried roots, bitter in taste and cold in nature, and returns to stomach meridian, large intestine meridian, liver meridian and spleen meridian. It has the effects of attacking stagnation, clearing damp and heat, purging fire and cooling blood, removing blood stasis and detoxifying. Indications of excess heat constipation, stagnant abdominal pain, dysentery, damp heat jaundice and other diseases. It has many functions such as purging, antibacterial, anti-tumor, anti-hyperlipidemia, lowering blood pressure, invigorating the stomach, promoting gallbladder, protecting the liver, strengthening the heart, anti-inflammatory, and regulating immunity. Tangut rhubarb is a variety of traditional Chines...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 陈垣郭凤霞白刚
Owner GANSU AGRI UNIV
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