Heavy metal bioremediation agent containing alga oligosaccharides and preparing method of agent

A technology of bioremediation agent and seaweed oligosaccharide, which is applied in the field of seaweed oligosaccharide-containing heavy metal bioremediation agent and its preparation, can solve the problems of different effects of heavy metals, poor stability of application effect, complicated preparation methods, etc., and achieve a beneficial effect on soil Microbial Activity, Harm Mitigation, Wide Range of Adaptive Effects

Active Publication Date: 2016-01-13
FUJIAN SANJU BIOLOGICAL SCI & TECH
View PDF9 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN102240670A provides a microbial remediation method for heavy metal-contaminated soil. The rhodobacter spheroides microbiological remediation agent can reduce the accumulation of heavy metals in plants to a certain extent, but when it is applied, the fermentation liquid is directly flooded with water to the heavy metal soil, and the shelf life is short. There are problems such as poor application effect stability; at the same time, the application rate is 50-200mL / kg soil, and the application rate converted into mu is 7500-30000L (the soil quality of one mu of cultivated layer is about 150000kg), which is a large amount and high cost
Chinese patent CN103275728A provides a kind of soil heavy metal biological fixative, adds four kinds of bacteria of Bacillus natto, Bacillus colloidus, Bacillus megaterium and Trichoderma konii in the organic fertilizer, the preparation method is complicated, and the cost is higher; and the adopted Although the strains have an adsorption effect on heavy metals, according to related reports, Bacillus megaterium and Bacillus colioids produce organic acids during the metabolic process, which can dissolve and activate soil heavy metals, increase the content of available heavy metals in soil, and promote plant absorption. Heavy metals (Yang Zhuo et al., 2009; Wang Xiaomin et al., 2013; Yang Rong et al., 2015); strains with different effects on heavy metals are compounded, resulting in a decrease in repair efficiency and an increase in cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Heavy metal bioremediation agent containing alga oligosaccharides and preparing method of agent
  • Heavy metal bioremediation agent containing alga oligosaccharides and preparing method of agent
  • Heavy metal bioremediation agent containing alga oligosaccharides and preparing method of agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 heavy metal bioremediation agent

[0032] 1. Preparation of Bacillus subtilis fermentation broth

[0033] 1) Plate culture: prepare nutrient agar plate (beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L, agar 20g / L, pH value before extinction is 7.3). Pick a ring with an inoculation loop from the Bacillus Subtilis Sanju-07 plate stored in a refrigerator at 4°C, streak culture on a nutrient agar plate, and culture at 30°C for 24 hours.

[0034] 2) Seed culture: prepare seed culture medium (beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L, pH value before sterilization is 7.0). Scrape off the activated Bacillus subtilis (Bacillus Subtilis) Triju-07 from the plate to make a bacterial suspension, and use 5% (v / v, bacterial suspension OD 600 =0.84) was inoculated into the seed medium, and cultured at 32°C and 200rpm for 24h.

[0035] 3) Fermentation culture: prepare fermentation medium (starch 30.0g / L, sucrose 5.0g / L, soybean mea...

Embodiment 2

[0046] Example 2 heavy metal bioremediation agent to Cd 2+ Adsorption

[0047] The experiment was designed with 4 experimental groups, which were blank control (treatment with CK, no addition), seaweed oligosaccharide (treatment A), heavy metal bioremediation without seaweed oligosaccharide (treatment B), heavy metal bioremediation with seaweed oligosaccharide ( For treatment group C), four concentration gradients (10, 20, 50, 100 g / L) were set for each treatment (A, B, C), and each experiment was repeated 3 times. According to the above experimental design, different additions of seaweed oligosaccharides, heavy metal bioremediation agents without seaweed oligosaccharides, and heavy metal bioremediation agents containing seaweed oligosaccharides were added with different concentrations of Cd 2+ In the solution, control the final volume of the solution to 10mL, so that Cd 2+ The final concentration is 50mg / L, 100mg / L, 150mg / L, shake at 28°C and 160rpm for 2h at constant tempe...

Embodiment 3

[0050] Example 3 The influence of heavy metal bioremediation agent on the germination of pakchoi seeds

[0051] Select pakchoi seeds with plump grains and the same size and disinfect them with 1% sodium hypochlorite for 15 minutes, rinse them with deionized water, and then soak them in deionized water for 12 hours. The pak choy seeds soaked in water were placed in a petri dish with two layers of filter paper, and 40 seeds were placed in each petri dish. Prepare different concentrations of Cd 2+ Solution 100mL (make the Cd in the solution 2+ Concentrations are 0, 5mg / L, 10mg / L, 20mg / L), take 10mL of the above solution into a petri dish with seeds, add 10mg of seaweed oligosaccharide (treatment A), no seaweed oligosaccharide heavy metal bioremediation agent (treatment B), heavy metal bioremediation agent containing seaweed oligosaccharides (treatment group C), and mixed evenly (3 repetitions for each treatment). Cover with plastic wrap, culture in an incubator at 27°C for 7 d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
degree of polymerizationaaaaaaaaaa
degree of polymerizationaaaaaaaaaa
Login to view more

Abstract

The invention discloses a heavy metal bioremediation agent containing alga oligosaccharides and a preparing method of the agent, and relates to the technical field of heavy metal bioremediation application. The heavy metal bioremediation agent containing the alga oligosaccharides comprises, by mass percentage, 5% to 15% of the alga oligosaccharides, 10% to 20% of bacillus subtilis fermented liquid and 65% to 85% of paecilomyces lilacinus solid leavening, wherein the effective viable count is larger than or equal to 2 hundred million CFU/g. The preparation method comprises the steps that the bacillus subtilis fermented liquid is prepared; the paecilomyces lilacinus solid leavening is prepared; and the alga oligosaccharides, the bacillus subtilis fermented liquid obtained in the first step and the paecilomyces lilacinus solid leavening obtained in the second step are mixed, and the heavy metal bioremediation agent containing the alga oligosaccharides is obtained. Bacillus subtilis and paecilomyces lilacinus have the higher tolerance on multiple kinds of heavy metal and can directly adsorb heavy metal ions, and the bacillus subtilis and the paecilomyces lilacinus do combined action with the alga oligosaccharides so as to be capable of passivating the heavy metal in the soil. Preparation is simple, the repairing effect on the heavy metal in the soil is good, and safety and environmental protection are achieved.

Description

technical field [0001] The invention relates to the technical field of application of heavy metal bioremediation, in particular to a heavy metal bioremediation agent containing seaweed oligosaccharides and a preparation method thereof. Background technique [0002] The problem of heavy metal pollution in my country is becoming more and more serious. The 2014 national land pollution survey report pointed out that some soils are seriously polluted, the soil environment quality of cultivated land is worrying, and the soil environment problems of industrial and mining wasteland are prominent. (Cd), Mercury (Hg), Copper (Cu), Lead (Pb), Chromium (Cr), Zinc (Zn), Nickel (Ni) and other 8 kinds of heavy metal pollution are the most serious, among which the heavy metal Cd point exceeding the standard rate reaches 7.0%, which is the highest rate of excessive heavy metal pollutants. Professor Zhao Qiguo, academician of the Chinese Academy of Sciences, pointed out at the "2015 Heavy Meta...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C12N1/20C12N1/14C12R1/125C12R1/79
Inventor 余劲聪林克明尤越
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap