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Method for preparing diosgenine by enzymatic hydrolysis

A technology of diosgenin and enzymolysis, which is applied in the field of enzyme engineering, can solve the problems of low yield of extraction method, complex process and the like, and achieves the effects of realizing zero discharge, simple operation process and solving water pollution.

Inactive Publication Date: 2016-01-13
遵义市倍缘化工有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at problems such as low yield and complex process of existing extraction methods, the purpose of the present invention is to provide a simple and feasible preparation method to improve the yield of diosgenin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Put 1g of turmeric powder in a 100ml Erlenmeyer flask, add 10mL of 0.2mol / L acetic acid-sodium acetate buffer solution, adjust the pH value to 4.0, add 0.01g of cellulase I, and enzymolyze at 40°C for 48 Hour. Add β-glucosidase I in an amount of 100 U / ml by liquid volume, and perform enzymatic hydrolysis at 40°C for 48 hours. Centrifuge under the condition of 5000r·min-1 for 15min to separate solid and liquid to obtain supernatant and solid residue. Dry the solid residue, add 50% ethanol at a solid-to-liquid ratio of 1:25, and extract at 50°C for 60 minutes. Crystallization yielded 31.68 mg of diosgenin.

Embodiment 2

[0026] Take 1g of turmeric powder and place it in a 100ml Erlenmeyer flask, add 20mL of acetic acid-sodium acetate buffer solution, adjust the pH value to 5.0, add 0.05g of cellulase I, and perform enzymolysis at 50°C for 38 hours under the condition of a water bath shaker. Add β-glucosidase I in an amount of 300 U / ml by liquid volume, and perform enzymatic hydrolysis at 45°C for 40 hours. The filtrate was removed by centrifugation to obtain a residue. Centrifuge under the condition of 5000r·min-1 for 15min to separate solid and liquid to obtain supernatant and solid residue. Dry the solid residue, add 60% ethanol at a solid-to-liquid ratio of 1:20, and extract at 60°C for 30 minutes. Crystallization gave 24.5 mg of diosgenin.

Embodiment 3

[0028] Take 1g of turmeric powder and put it in a 100ml Erlenmeyer flask, add 30mL of acetic acid-sodium acetate buffer solution, adjust the pH value to 6.0, add 0.1g of cellulase I, and perform enzymatic hydrolysis at 55°C for 24 hours under the condition of a water bath shaker. Add β-glucosidase I in an amount of 600 U / ml by liquid volume, and perform enzymatic hydrolysis at 50°C for 30 hours. The filtrate was removed by centrifugation to obtain a residue. Centrifuge under the condition of 5000r·min-1 for 15min to separate solid and liquid to obtain supernatant and solid residue. Dry the solid residue, add 70% ethanol at a solid-to-liquid ratio of 1:15, and extract at 70°C for 20 minutes. Crystallization yielded 31.3 mg of diosgenin.

[0029] This process can achieve a maximum yield of diosgenin of 31.68 mg / g and a co-production of glucose of 230 mg / g. For the first time, this process completely uses commercial enzymes to convert diosgenin in turmeric into diosgenin, and ...

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Abstract

The invention relates to a preparation method of diosgenine. The preparation method is a method for preparing diosgenine by using dioscorea zingiberensis as a raw material through enzymatic hydrolysis and belongs to the field of enzyme engineering. The method for preparing diosgenine by enzymatic hydrolysis comprises the following steps: immersing crushed dioscorea zingiberensis in an acetic acid-sodium acetate buffer solution to successively carry out cellulase I enzymolysis and beta-glucosidase I enzymolysis, collecting enzymolysis residue and purifying to prepare diosgenine. The method for extraction of diosgenine has a simple extraction technology, has little environmental pollution and is suitable for large-scale popularization.

Description

technical field [0001] The invention relates to a method for preparing diosgenin, which is a method for preparing diosgenin by using turmeric as a raw material through enzymatic hydrolysis, and belongs to the field of enzyme engineering. Background technique [0002] Diosgenin, referred to as saponin, also known as diosgenin, diogenin, English name diosgenin (CASNo.512-04-9), is an important precursor compound for the synthesis of steroid hormones and steroidal contraceptives. Known as "medicinal gold" and "mother of hormones". Dioscin is modified as the starting parent, and can synthesize as many as 300 compounds such as sex hormones, adrenal cortex hormones and other steroids. In addition to being a drug precursor, diosgenin itself also has biological activities and medicinal functions such as antibacterial, antiviral, antithrombotic, anti-osteoporosis, promotion of bone formation, treatment of cardiovascular diseases, diabetes, anti-cancer, and anti-oxidation. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12P33/20
Inventor 袁其朋王双闫美屹张基明
Owner 遵义市倍缘化工有限责任公司
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