Method for preparing high-purity chlorogenic acid from eucommia ulmoides leaf crude extract

A technology of crude extract and Eucommia leaves, which is applied in the field of preparation of high-purity chlorogenic acid, can solve the problems that have not been reported, and achieve the effect of promoting rational utilization, simple and feasible process, and good economic benefits

Active Publication Date: 2016-01-20
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After reviewing the literature, we learned that membrane separation technology, macroporous resin separation and purification technology and activated carbon decolorization technology have all been used in the preparation of chlorogenic acid, but the combination of the three technologies has not been reported in the research of preparation of high-purity chlorogenic acid.

Method used

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  • Method for preparing high-purity chlorogenic acid from eucommia ulmoides leaf crude extract
  • Method for preparing high-purity chlorogenic acid from eucommia ulmoides leaf crude extract

Examples

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Embodiment 1

[0033] Add 100g Eucommia leaves crude extract, add 500ml water to fully dissolve, stir and prepare the Eucommia leaves crude extract aqueous solution, adjust the pH of the aqueous solution to 4 with a pH meter, first pass the above aqueous solution through an ultrafiltration membrane with a molecular weight cut-off of 1000 for membrane separation, collect Permeate, pass the permeate through NKA-9 macroporous resin at a flow rate of 10BV / h to fully absorb to saturation, and rinse the above macroporous resin column with deionized water at a flow rate of 3BV / h until the effluent is colorless At this time, the NKA-9 type macroporous resin A column and the ADS-8 type macroporous resin B column are connected in series, and then 60% ethanol solution is used to pass through the A column and the B column at a flow rate of 8BV / h for elution and removal. impurity, collect the eluate obtained after impurity removal to high performance liquid chromatography (HPLC) to detect that there is no...

Embodiment 2

[0035]Add 400g of eucommia leaf crude extract, add 4000ml of deionized water to fully dissolve, stir and prepare an aqueous solution of eucommia leaf crude extract, use a pH meter to adjust the pH of the aqueous solution to 4, and pass the above aqueous solution through an ultrafiltration membrane with a molecular weight cut-off of 1000 for membrane separation , collect the permeate, pass the permeate through the ADS-7 macroporous resin at a flow rate of 8BV / h for dynamic adsorption to saturation, and rinse the above macroporous resin column with deionized water at a flow rate of 2BV / h to the effluent Colorless, at this time, connect ADS-7 type macroporous resin column and HPD-300 type macroporous resin column in series first, then use ethanol solution with a volume fraction of 50% to flow through the adsorption column ADS-7 at a flow rate of 7BV / h Type macroporous resin column and impurity removal column HPD-300 type macroporous resin column for elution and impurity removal, c...

Embodiment 3

[0037] 1000g Eucommia leaf crude extract, add 10000ml deionized water to fully dissolve, stir to prepare the Eucommia leaf crude extract aqueous solution, adjust the pH of the aqueous solution to 4 with a pH meter, and pass the above aqueous solution through an ultrafiltration membrane with a molecular weight cut-off of 1000 for membrane separation , collect the permeate, pass the permeate through the ADS-F8 macroporous resin at a flow rate of 5BV / h to absorb to saturation, rinse the above macroporous resin column with deionized water at 6BV / h until the effluent is colorless, At this time, the ADS-F8 type macroporous resin column and the BS-65 type macroporous resin are connected in series, and then the ethanol solution with a volume fraction of 60% is used to pass through the above-mentioned series of columns successively at a flow rate of 8BV / h for elution and removal of impurities. , collect the ethanol eluate and carry out membrane concentration through a nanofiltration mem...

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Abstract

The invention relates to a method for preparing high-purity chlorogenic acid, in particular to a method for preparing high-purity chlorogenic acid from eucommia ulmoides leaf crude extract. The method specifically includes the steps of sufficiently dissolving eucommia ulmoides leaf crude extract as the raw material in deionized water to obtain an eucommia ulmoides leaf crude extract aqueous solution, conducting separation through a membrane, collecting permeate, conducting adsorption and impurity removal on permeate through resin adsorption columns connected in series, collecting and concentrating eluent, refining a concentrated solution through activated carbon, conducting freezing and drying to obtain high-purity chlorogenic acid. The method is characterized in that a negative and position exchange column device is adopted, the adsorption, washing and impurity removal operation procedures are completed in the same device, and time and operation steps are saved; in addition, the raw material is processed through a large device in the preparation method, lots of samples can be processed at a time, the process is simple and feasible, operation is convenient, the agent is green, free of pollution and capable of being recycled, activated carbon and large-hole resin can be recycled, cost is low, and high-purity chlorogenic acid can be industrially produced and has high social benefits, economic benefits and environment benefits.

Description

technical field [0001] The invention relates to a method for preparing high-purity chlorogenic acid, in particular to a method for preparing high-purity chlorogenic acid from Eucommia leaves crude extract, belonging to the field of natural product purification. Background technique [0002] Eucommia ulmoides (Eucommia ulmoides Oliv.) is a plant of the genus Eucommia in the Eucommia family. It is a unique economic tree species in my country, mainly distributed in Gansu, Zhejiang, Henan, Guangxi, Sichuan, Yunnan and other regions. Eucommia ulmoides is a traditional precious Chinese herbal medicine, which has the functions of curing diseases and health care without diseases. In "Shen Nong's Materia Medica", eucommia is listed as top grade. Its medicinal value has always attracted attention, and it has pharmacological effects such as invigorating the middle and nourishing qi, strengthening muscles and bones, nourishing the liver and kidney, anti-fatigue, anti-abortion, lowering ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48
CPCY02P20/582C07C67/48C07C69/732
Inventor 夏传海苗芹孙鹏程
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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