Method for efficiently removing epibiotic microorganisms on surfaces of large seaweed

A technology of microorganisms and seaweeds, applied in the direction of fungi and bacteria, can solve the problems of killing endophytic microorganisms, affecting the diversity composition of endosymbiotic microorganisms, killing, etc., and achieves the effects of efficient removal, easy mass production, and low cost

Inactive Publication Date: 2016-01-27
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the epiphytic microorganisms of seaweed do not simply attach to the cell surface, but establish a relatively close connection with the host cell through a macromolecular network structure such as polysaccharides, proteins, and nucleic acids, it is difficult to effectively remove them with conventional mild methods and will Periphytic microorganisms
For example, physical methods such as co-oscillation of glass beads and algae, ultrasonic treatment, etc. have little effect on endophytic microorganisms, but studies have shown that they cannot completely rem

Method used

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  • Method for efficiently removing epibiotic microorganisms on surfaces of large seaweed
  • Method for efficiently removing epibiotic microorganisms on surfaces of large seaweed

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: The quartz sand method thoroughly removes the epiphytic microorganisms of the large green alga Enteromorpha (U.prolifera)

[0024] Enteromorpha is a single-layer tubular algal body, which is relatively fragile. In addition to the quartz sand method of the present invention, a total of 12 methods of enzymatic method, chemical method and physical method were compared for the removal effect of fresh Enteromorpha epiphytic microorganisms. Scanning electron microscopy was used to determine whether the epiphytic microorganisms of Enteromorpha were removed, and the effects of various methods on the endophytic microorganisms of Enteromorpha were detected by deformable gradient gel electrophoresis (DGGE).

[0025] Test 7 kinds of enzymatic methods altogether, at first prepare the lysozyme of 2mg / ml, papain, helicase, cellulase, lysozyme+papain+helicase (every kind of enzyme concentration is 2mg / ml) and proteinase K, more Enzyme cleaners were purchased from finishe...

Embodiment 2

[0031] Embodiment 2: The quartz sand method thoroughly removes the epiphytic microorganisms of the large green alga Enteromorpha flatus (U.compressa)

[0032] Take about 0.03g of Enteromorpha algae and 0.3g of the above-mentioned quartz sand with a particle size range of 125-250μm and mix them in a 1.5ml Eppendorf centrifuge tube, add 1ml of sterilized seawater, and shake at 3200rpm for 2×15min to obtain the epiphytic The ideal result that the microorganisms have been completely eliminated without affecting the algal cells ( figure 1 , r).

Embodiment 3

[0033] Embodiment 3: Quartz sand method thoroughly removes the epiphytic microorganisms of large-scale green algae Enteromorpha (U.linza)

[0034] Take about 0.03g of Enteromorpha algae and 0.3g of the above-mentioned quartz sand with a particle size range of 125-250μm and mix them in a 1.5ml Eppendorf centrifuge tube, add 1ml of sterilized seawater, shake at 3200rpm for 2×15min, and obtain the attached The ideal result that the living microorganisms have been completely removed and has no effect on the algal cells ( figure 1 , s).

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Abstract

The invention belongs to the field of biotechnologies of seaweed, and relates to a method for efficiently removing epibiotic microorganisms on the surfaces of large seaweed. The method particularly includes removing the epibiotic microorganisms on the surfaces of the to-be-treated large seaweed under the resonance actions of quartz sand and the to-be-treated large seaweed. The method has the advantages that the epibiotic microorganisms on the surfaces of the large seaweed can be efficiently and thoroughly removed by the aid of the method, and the method is free of interference on endophytic microorganisms; the method can be used for separating and purifying endosymbiotic microorganisms of the seaweed and studying active natural products of the seaweed.

Description

technical field [0001] The invention belongs to the technical field of seaweed biology and relates to a method for efficiently removing epiphytic microorganisms on the surface of large seaweeds. Background technique [0002] Macroalgae belong to lower plants, mainly including brown algae, red algae and green algae. Many of these economic species are the objects of artificial cultivation, such as seaweed (Saccharinas pp.), wakame (Undaria pinnatifida), hijiki (Sargassumfusiforme) of brown algae, seaweed (Pyropias pp.) of red algae, red cabbage (Bangias pp.), Gracilaria spp., Gelidium spp., Eucheumas pp., and green algae Enteromorpha and Ulvas pp. [0003] Large seaweed can not only be used as healthy food (such as seaweed, red cabbage, kelp, wakame, enteromorpha), but also be used to extract and prepare important chemical raw materials such as iodine, mannitol, and alginate. In addition, recent studies have found that macroalgae are also important hosts of marine microorgan...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14
Inventor 姜鹏刘晓杰赵瑾陈华新
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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