High sensitivity LSPR (Localized Surface Plasmon Resonance) biochemical sensing kit and application method

Abstract

The present invention relates to a LSPR (Localized Surface Plasmon Resonance) biochemical sensing kit and an application method, and the LSPR biochemical sensing kit can be used for detecting a low concentration to-be-tested matter in a simple, fast and inexpensive way. The kit contains a plasma metal nanostructure substrate and a bare gold particle suspension solution, and the plasma metal nanostructure substrate comprises a substrate, single-layer nano metal particles embedded thereon, and a receiver which is modified thereon. The application method of the kit in detection of the low concentration to-be-tested matter is as follows: the single-layer nano metal particles are embedded on the nano plasma wafer substrate by a heating method, the receiver is modified on the wafer surface, then the to-be-tested matter is modified, meanwhile bare gold particles are added dropwise to modify on the to-be-tested matter, and a LSPR signal of the to-be-tested matter is detected. By the sensing kit and the application method, a simple way is used to modify the bare gold particles on the to-be-tested matter to enhance the LSPR signal so as to reach the purpose and effect of detection of the low concentration to-be-tested matter, and the sensing kit and the application method have the high value of innovative industry application.

Description

technical field [0001] The invention relates to an LSPR biochemical sensing kit and an application method thereof. More particularly, the present invention relates to a method for enhancing LSPR signal by modifying bare gold particles on the analyte during LSPR analysis. Background technique [0002] The principle of localized surface plasmon resonance (LSPR) is that when metal nanoparticles are fabricated on a transparent substrate, they are excited by incident light, which will cause surface plasmon resonance on the surface of the metal nanoparticles. The intensity is easily affected by the surrounding environment, resulting in a shift in wavelength or a change in signal intensity. Therefore, the change in local dielectric constant can be used to detect analytes. Therefore, as long as there is an analyte bound near the particle, we can measure the optical change through optical instruments. It can be imagined that the surface of nanoparticles is like a tiny detector, whi...

AI Technical Summary

Problems solved by technology

However, the OES technology itself must use the labeled secondary antibody, so it has lost the advantage of the original LSPR without labeling (label-free)

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