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Application of a hydrogel microsphere embedded with coagulation-related enzymes

A technology for hydrogel microspheres and coagulation, which is applied in the directions of medical preparations without active ingredients, medical preparations containing active ingredients, and preparation of microspheres, and achieves the effect of mild process conditions and easy maintenance of biological activity.

Active Publication Date: 2019-05-07
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if it is a lumen system with a large diameter and positive pressure propulsion, it is difficult to achieve rapid coagulation of fluid blood (or plasma) and effective closure of the target lumen in a short period of time.

Method used

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  • Application of a hydrogel microsphere embedded with coagulation-related enzymes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 【1】Preparation of sodium alginate solution (concentration 15g / L);

[0035] [2] Dissolve thrombin (100 mg) into the sodium alginate solution prepared in step [1] at a concentration of 10 g / L to obtain a sodium alginate solution containing thrombin;

[0036] [3] Prepare the gel bath solution, which contains calcium chloride (concentration 30g / L), sodium chloride (concentration 5g / L), sodium dihydrogen phosphate (concentration 1g / L), Tween 5g / L in the gel bath ;

[0037] [4] Under the high-voltage electrostatic field, the sodium alginate solution containing thrombin prepared in step [2] is formed into uniform jet droplets, and the droplets enter the gel bath solution prepared in step [3] to gel. Reaction, reacting for 30 minutes to obtain alginate hydrogel microspheres embedded with thrombin, the particle size of the microspheres is 500±100 microns.

[0038] [5] The thrombin-embedded alginate hydrogel microspheres prepared in step [4] were reacted with chitosan solution ...

Embodiment 2

[0050] 【1】Preparation of sodium alginate solution (concentration 20g / L);

[0051] [2] Dissolve thrombin (50 mg) into the sodium alginate solution prepared in step [1] at a concentration of 10 g / L to obtain a sodium alginate solution containing thrombin;

[0052] [3] Prepare the gel bath solution, which contains barium chloride (concentration 20g / L), sodium chloride (concentration 1g / L), sodium dihydrogen phosphate (concentration 1g / L), Tween 10g / L in the gel bath ;

[0053] [4] Under a high-voltage electrostatic field, the sodium alginate solution containing thrombin prepared in step 2 is formed into uniform jet droplets, and the droplets enter the gel bath solution prepared in step [3] to undergo a gelation reaction. After reacting for 30 minutes, the alginate hydrogel microspheres embedded with hemocoagulase were obtained, and the particle size of the microspheres was 300±100 microns.

[0054] [5] The thrombin-embedded alginate hydrogel microspheres prepared in step [4] we...

Embodiment 3

[0059] [1] According to the steps [1-4] of Example 1, alginate hydrogel microspheres embedded with thrombin were prepared.

[0060] 【2】Animal model preparation: New Zealand white rabbits were anesthetized by intraperitoneal injection, skin was prepared in the groin, an arterial sheath was implanted, and a contrast catheter was used to reach the branch of the renal artery (target artery) through the femoral artery and abdominal aorta.

[0061] 【3】Open the abdomen to expose the kidney, and make a model of hemorrhage injury.

[0062] [4] After the contrast catheter was delivered to the embolization site at the proximal end of the injured vessel, alginate microspheres embedded with thrombin were injected slowly (thrombin alginate microspheres: contrast agent: 0.9% normal saline = 1: 1:1).

[0063] 【5】Observe the changes of the bleeding at the injured part of the kidney after embolization. It was found that the bleeding stopped within 5 seconds after embolization. After complete h...

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Abstract

The invention discloses application of hydrogel microspheres embedded with blood coagulation related enzyme. Grain size of the prepared microspheres is 100-1000 micrometers and is uniform (CV is smaller than 20%), the blood coagulation related enzyme is uniformly distributed in the hydrogel microspheres, a mass ratio of the blood coagulation related enzyme to sodium alginate in the microspheres is 1:3-4:1, drug loading rate of the dewatered blood coagulation related enzyme is 25-80%, and the hydrogel microspheres carrying the blood coagulation related enzyme are used for coagulation of blood and plasma.

Description

technical field [0001] The invention relates to the application of a hydrogel microsphere, in particular to the application of a hydrogel microsphere embedded with coagulation-related enzymes in blood coagulation. Background technique [0002] Currently disclosed methods for coagulation of blood (or plasma) all use coagulation-related enzymes for direct coagulation, and this method is only suitable for coagulation of a small amount of static samples or relatively small pipelines. However, if it is a lumen system with a large diameter and positive pressure propulsion, it is difficult to achieve rapid coagulation of flowing blood (or plasma) and effective closure of the target lumen in a short period of time. Contents of the invention [0003] In view of the above problems, the present invention combines the biochemical procoagulant activity of thrombin with the blocking effect of hydrogel microspheres, and proposes the application of hydrogel microspheres embedded with coag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/48A61K9/16A61K47/36B01J13/02A61P7/04
Inventor 韩雅玲马小军荣晶晶于炜婷孙景阳梁明
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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