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Application of hydrogel microspheres embedded with blood coagulation related enzyme

A technology for hydrogel microspheres and coagulation, which is applied in the directions of medical preparations without active ingredients, medical preparations containing active ingredients, and preparation of microspheres, and achieves the effect of mild process conditions and easy maintenance of biological activity.

Active Publication Date: 2016-02-03
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if it is a lumen system with a large diameter and positive pressure propulsion, it is difficult to achieve rapid coagulation of fluid blood (or plasma) and effective closure of the target lumen in a short period of time.

Method used

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  • Application of hydrogel microspheres embedded with blood coagulation related enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 【1】Prepare sodium alginate solution (concentration 15g / L);

[0035] [2] Dissolve thrombin (100 mg) into the sodium alginate solution prepared in step [1] at a concentration of 10 g / L to obtain a sodium alginate solution containing thrombin;

[0036] 【3】Prepare gel bath solution, the gel bath contains calcium chloride (concentration 30g / L), sodium chloride (concentration 5g / L), sodium dihydrogen phosphate (concentration 1g / L), Tween 5g / L ;

[0037] [4] Under a high-voltage electrostatic field, the sodium alginate solution containing thrombin prepared in step [2] is formed into uniform jet droplets, and the droplets enter the gel bath solution prepared in step [3], and gelation occurs After 30 minutes of reaction, the alginate hydrogel microspheres embedded with thrombin are obtained, and the particle size of the microspheres is 500±100 microns.

[0038] [5] The thrombin-embedded alginate hydrogel microspheres prepared in step [4] were reacted with chitosan solution (concentrati...

Embodiment 2

[0050] 【1】Prepare sodium alginate solution (concentration 20g / L);

[0051] [2] Dissolve thrombin (50 mg) into the sodium alginate solution prepared in step [1], at a concentration of 10 g / L, to obtain a sodium alginate solution containing thrombin;

[0052] 【3】Prepare gel bath solution, the gel bath contains barium chloride (concentration 20g / L), sodium chloride (concentration 1g / L), sodium dihydrogen phosphate (concentration 1g / L), Tween 10g / L ;

[0053] [4] Under a high-voltage electrostatic field, the sodium alginate solution containing thrombin prepared in step 2 is formed into uniform jet droplets, and the droplets enter the gel bath solution prepared in step [3], and a gelation reaction occurs. After 30 minutes of reaction, the alginate hydrogel microspheres embedded with hemagglutinin are obtained, and the particle size of the microspheres is 300±100 microns.

[0054] [5] The alginate hydrogel microspheres embedded with thrombin prepared in step [4] are reacted with polylysine...

Embodiment 3

[0059] [1] Prepare the alginate hydrogel microspheres embedded with thrombin according to the steps [1-4] of Example 1.

[0060] [2] Preparation of the animal model: New Zealand white rabbits were intraperitoneally injected anesthetized, the skin was prepared in the groin, the arterial sheath was implanted, and the angiographic catheter was used to reach the renal artery branch (target artery) through the femoral artery and abdominal aorta.

[0061] [3] Open the abdomen to expose the kidney and make a bleeding injury model.

[0062] [4] The contrast catheter is delivered to the embolization site at the proximal end of the injured blood vessel and then slowly injects the alginate microspheres embedded with thrombin (thrombin alginate microspheres: contrast agent: 0.9% saline = 1: 1:1).

[0063] [5] Observe the changes in the bleeding at the kidney injury after embolization. It was found that the bleeding stopped 5 seconds after embolization. After the bleeding was completely stopped an...

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Abstract

The invention discloses application of hydrogel microspheres embedded with blood coagulation related enzyme. Grain size of the prepared microspheres is 100-1000 micrometers and is uniform (CV is smaller than 20%), the blood coagulation related enzyme is uniformly distributed in the hydrogel microspheres, a mass ratio of the blood coagulation related enzyme to sodium alginate in the microspheres is 1:3-4:1, drug loading rate of the dewatered blood coagulation related enzyme is 25-80%, and the hydrogel microspheres carrying the blood coagulation related enzyme are used for coagulation of blood and plasma.

Description

Technical field [0001] The invention relates to the application of hydrogel microspheres, in particular to the application of hydrogel microspheres embedded with coagulation-related enzymes in blood coagulation. Background technique [0002] The currently disclosed methods for blood (or plasma) coagulation all use the direct coagulation of coagulation-related enzymes, and this method is only suitable for the coagulation of a small amount of static samples or relatively small tubes. However, if it is a lumen system with a large diameter and a positive pressure is applied, it is difficult to achieve rapid coagulation of fluid blood (or plasma) and effective sealing of the target lumen in a short time. Summary of the invention [0003] In view of the above problems, the present invention combines the biochemical coagulation-promoting activity of thrombin with the blocking effect of hydrogel microspheres, and proposes the application of hydrogel microspheres embedded with coagulation-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61K9/16A61K47/36B01J13/02A61P7/04
Inventor 韩雅玲马小军荣晶晶于炜婷孙景阳梁明
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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