Method for quickly extracting and separating lipid components in peony seeds

Abstract

The invention discloses a method for quickly extracting and separating lipid components in peony seeds. The method is characterized by loading a total lipid sample to a solid-phase extraction silica gel column through extracting total lipids in the peony seeds; then respectively carrying out elution by using chloroform, acetone and methanol as elution solutions to obtain neutral lipids, glycolipids and phospholipids; then respectively and further expanding the neutral lipids, the glycolipids and the phospholipids, separating triacylglycerol from the neutral lipids, separating MGDG (Monogalactosyl Diacylglycerol), DGDG (Digalactosyl Diglyceride) and SQDG (Sulfoquinovosyl Diacylglycerol) from the glycolipids, and separating phosphatidylethanolamine, phosphatidylcholine, phosphatidic acid, phosphatidylserine and phosphatidylglycerol from the phospholipids. According to the method disclosed by the invention, nine kinds of lipid components in grease of the peony seeds can be effectively separated, the variety of the obtained lipid components is rich, and fatty acid can be obtained after carrying out methyl esterification on the total lipids, the neutral lipids, the glycolipids and the phospholipids; the method is quick and simple, the cost is low, and a feasible analysis method is provided for fundamental researches.

Description

technical field [0001] The invention belongs to the field of woody plant oil crops, in particular to a method for rapidly extracting and separating lipid components in peony seeds, which can quickly and efficiently extract lipid components in peony seeds with only a small amount of seeds, and further separate the obtained total lipids , and analyze its components and content. Background technique [0002] The rich and auspicious peony, known as the "national flower", is an important part of Chinese traditional culture. Its root bark can be used as medicine, and its seed oil is rich in unsaturated fatty acids, especially α-linolenic acid (ALA, 18 :3 Δ9,12,15 ), making peony have both ornamental, medicinal and oil values. In recent years, the development of polyunsaturated fatty acids has become one of the hot spots in functional food research, while the content of saturated fatty acids in peony seed oil is very low, and the content of unsaturated fatty acids is very high. ...

AI Technical Summary

Problems solved by technology

[0006] The traditional extraction method of peony seed oil is Soxhlet extraction, and fat extractors are often used in laboratories for extraction, but this method not only requires a large number of samples but also takes a long time, generally taking several hours to more than ten hours

With the continuous improvement of vegetable seed oil extraction technology, especially the application of Soxhlet extraction, supercritical fluid extraction technology and ultrasonic and microwave extraction methods, the extraction rate of vegetable seed oil has been greatly improved, but these extraction methods require a large amount of samples. , it is difficult to meet the extraction requirements for samples with scarce resources

Images