Method for quickly extracting and separating lipid components in peony seeds

A peony seed, fast technology, applied in the direction of fat production, fat oil/fat production, fat oil/fat refining, etc., can solve the problem that the samples of scarce resources are difficult to meet the extraction requirements, hours to ten hours, large sample volume, etc. problems, to achieve the effects of delamination, less application materials, and fast operation methods
CN105296142AActive Publication Date: 2016-02-03SHANGHAI CHENSHAN BOTANICAL GARDEN +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI CHENSHAN BOTANICAL GARDEN
Publication Date
2016-02-03

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Abstract

The invention discloses a method for quickly extracting and separating lipid components in peony seeds. The method is characterized by loading a total lipid sample to a solid-phase extraction silica gel column through extracting total lipids in the peony seeds; then respectively carrying out elution by using chloroform, acetone and methanol as elution solutions to obtain neutral lipids, glycolipids and phospholipids; then respectively and further expanding the neutral lipids, the glycolipids and the phospholipids, separating triacylglycerol from the neutral lipids, separating MGDG (Monogalactosyl Diacylglycerol), DGDG (Digalactosyl Diglyceride) and SQDG (Sulfoquinovosyl Diacylglycerol) from the glycolipids, and separating phosphatidylethanolamine, phosphatidylcholine, phosphatidic acid, phosphatidylserine and phosphatidylglycerol from the phospholipids. According to the method disclosed by the invention, nine kinds of lipid components in grease of the peony seeds can be effectively separated, the variety of the obtained lipid components is rich, and fatty acid can be obtained after carrying out methyl esterification on the total lipids, the neutral lipids, the glycolipids and the phospholipids; the method is quick and simple, the cost is low, and a feasible analysis method is provided for fundamental researches.
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Description

technical field

[0001] The invention belongs to the field of woody plant oil crops, in particular to a method for rapidly extracting and separating lipid components in peony seeds, which can quickly and efficiently extract lipid components in peony seeds with only a small amount of seeds, and further separate the obtained total lipids , and analyze its components and content. Background technique

[0002] The rich and auspicious peony, known as the "national flower", is an important part of Chinese traditional culture. Its root bark can be used as medicine, and its seed oil is rich in unsaturated fatty acids, especially α-linolenic acid (ALA, 18 :3 Δ9,12,15 ), making peony have both ornamental, medicinal and oil values. In recent years, the development of polyunsaturated fatty acids has become one of the hot spots in functional food research, while the content of saturated fatty acids in peony seed oil is very low, and the content of unsaturated fatty acids is very high. ...

Claims

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