Maize yellow pigment extracting technology

A technology of corn yellow pigment and extraction process, which is applied in the field of natural product extraction, can solve the problems of complex operation and difficult industrial production of corn yellow pigment, and achieve the effects of easy industrial transformation, improved extraction rate, and simple process

Inactive Publication Date: 2016-02-03
丁春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the above problems that the operation of extracting zeaxanthin from zein is complicated and it is difficult to realize industrial production, the present invention provides a high extraction rate, simple and easy to operate The method for extracting zeaxanthin from zein

Method used

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Embodiment approach

[0027] Hereinafter, the present invention will be further described with examples, but it is not limited to any one of these examples or similar examples.

[0028] In order to better understand the present invention, the following will be further described in conjunction with specific examples.

Embodiment 1

[0030] (1) Ultrafinely pulverize corn gluten powder into 300-mesh superfine powder, weigh 2g of zein superfine powder in the reaction device, add deionized water, control the mass concentration of superfine powder to 4%, and add 0.3% of superfine powder mass Xylanase with an enzyme activity of 15,000IU / g and Aspergillus oryzae protease with an enzyme activity of 60,000IU / g accounting for 0.8% of the ultrafine powder mass were enzymatically hydrolyzed at 42±5°C for 4 hours, the enzymatic hydrolyzate was centrifuged, and the supernatant was discarded , have to precipitate;

[0031] (2) Add ethanol to the precipitate in step (1), add 24mL of 90% ethanol according to the ratio of solid to liquid 1:12, and extract in a microwave reaction system with a microwave power of 220W, a time of 115s, and a temperature of 42±5°C. After the reaction is completed, centrifuge at a high speed of 10000r / min for 10min, and collect the supernatant;

[0032] (3) The supernatant was stored at -18°C ...

Embodiment 2

[0035] (1) Ultrafinely pulverize corn gluten powder into 500-mesh superfine powder, weigh 2g of zein superfine powder in the reaction device, add deionized water, control the mass concentration of superfine powder to 5%, and add 0.5% of superfine powder mass Xylanase with an enzyme activity of 15,000IU / g and Aspergillus oryzae protease with an enzyme activity of 60,000IU / g accounting for 1.2% of the ultrafine powder mass were enzymatically hydrolyzed at 42±5°C for 5h, the enzymatic hydrolyzate was centrifuged, and the supernatant was discarded , have to precipitate;

[0036] (2) Add ethanol to the precipitate in step (1), add 30mL of 95% ethanol according to the ratio of solid to liquid 1:15, and extract in a microwave reaction system with a microwave power of 480W, a time of 115s, and a temperature of 42±5°C. After the reaction is completed, centrifuge at a high speed of 10000r / min for 10min, and collect the supernatant;

[0037] (3) The supernatant was stored at -18°C for 1...

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Abstract

The invention relates to the technical field of deep processing of maize protein powder, in particular to a method for extracting maize yellow pigment from maize protein. The method includes: subjecting the maize protein powder to superfine grinding to obtain 300-500-mesh superfine powder, adding water, xylanase and aspergillus oryzae protease for enzymolysis, centrifuging enzymatic hydrolysate and getting rid of supernatant so as to obtain precipitate; adding ethanol into the precipitate, extracting in a microwave reaction system and collecting supernatant; removing protein from the supernatant, subjecting filtrate to concentration under reduced pressure to obtain paste and subjecting the paste to vacuum freeze drying till constant weight is reached. The method has the advantages that the xylanase and the aspergillus oryzae protease are used for enzymolysis of superfine maize protein powder so as to facilitate increase of maize yellow pigment extracting ratio; superfine grinding is more beneficial to dissolution of the maize yellow pigment during extraction.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting zeaxanthin from zein. Background technique [0002] Zeaxanthin is a mixture of several carotenoids, of which dihydroxycarotene accounts for about 81.2%, monohydroxycarotene accounts for 17.2%, and carotene accounts for about 1.6%. In 1962, Blessin confirmed that zeaxanthin exists in a complex form with protein in corn. Its content varies with different types of corn and protein content. The content of zeaxanthin in corn gluten powder reported at home and abroad is mostly between 300μg / g-700μg / g. Among them, multiple conjugated double bonds in hydroxycarotene make it have a bright color. Lutein is not a precursor of VA, and it cannot be transformed even after being absorbed by the intestinal tract, so it can maintain its own characteristics to the greatest extent. Therefore, most of the domestic processing of corn gluten meal is used as...

Claims

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Application Information

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IPC IPC(8): C12P23/00
Inventor 丁春燕
Owner 丁春燕
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