Preserving fluid and preparation method thereof

A technology of preservation solution and carrageenan, which is applied in the field of chemiluminescence immunoassay, can solve the problems of affecting test results, unfavorable stable storage of markers on particles, and affecting antibody immune response

Inactive Publication Date: 2016-02-03
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, with the rapid development of chemiluminescence immunoassay technology, many shortcomings have also been exposed. One of them is the preservation of magnetic particles and magnetic nanoparticles. If the viscosity of the preservation solution of magnetic particles or magnetic nanoparticles in the test strip is too low, First of all, magnetic particles or magnetic nanoparticles are prone to agglomeration and sinking to the bottom, which is not conducive to the stable storage of markers on

Method used

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  • Preserving fluid and preparation method thereof
  • Preserving fluid and preparation method thereof

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[0031] The present invention also provides a preparation method of the above-mentioned preservation solution, which comprises: mixing 0.1-10 g / L buffer salt, 100-300 g / L antibody stabilizer, 0.1-1 g / L antiseptic and antibacterial agent, and 2-12 g / L caramel The glue is mixed with water and heated to dissolve to obtain a preservation solution.

[0032] According to the present invention, it is preferable to add a thickening agent other than carrageenan at 1-100 g / L. Wherein, the buffer salt, antibody stabilizer, antiseptic and antibacterial agent, carrageenan, water and thickeners other than carrageenan are all the same as those described above, and will not be repeated here.

[0033] In the present invention, it is preferable to first mix 0.1-10g / L buffer salt, 100-300g / L antibody stabilizer, 0.1-1g / L antiseptic and bacteriostatic agent, 2-12g / L carrageenan with part of water, and the mixing The method is preferably ultrasonic or stirring, heating to dissolve, and then adding the ...

Example Embodiment

[0038] Example 1

[0039] Add 700ml sterile water to a 1000ml conical flask, and then add 1.8g Na 2 HPO 4 ·12H 2 O, 0.22gKH 2 PO 4 , 3gNaCl, 0.3gKCl, 50gBSA, 150g glycerol, 0.3g sodium azide and 0.5g Proclin300, ultrasonically mixed, filtered, heated in a water bath at 40℃, and added 3g polyethylene glycol 12000, 6g carrageenan kappa type, 1g alginic acid in batches Sodium and 1g polyvinyl alcohol are completely dissolved, and then add water to make the volume to 1000ml to obtain the preservation solution.

[0040] Disperse the C-reactive protein-coated magnetic nanoparticles evenly into the preservation solution.

[0041] Performance test: Use a pipette to accurately pipette 100μl, 0.1mol / L phosphate buffer (PB6.5) with a pH of 6.5 to a 1.5mL centrifuge tube, and add 10μl 0.1μg / ml CRP antigen or 1μg / ml CRP antigen, 20μl of alkaline phosphatase-labeled antibody and 100μl of storage solution dispersed with magnetic nanoparticles, then use a pipette to disperse the mixture evenly, and...

Example Embodiment

[0048] Example 2

[0049] Add 700ml of sterilized water to a 1000ml Erlenmeyer flask, then add 2.3gMOPS, 0.19gNaOH, 2.2gNaCl, 1.3gKCl, 60g calf serum, 140g glycerol, 0.3g sodium azide and 0.2g chloramphenicol, and mix with ultrasound. , Filter, heat in a water bath at 40°C, add 5g polyvinylpyrrolidone 360, 3g carrageenan λ type, 3g carrageenan type, 1g gellan gum and 1g polyvinyl alcohol in batches until completely dissolved, and then add water to make the volume to 1000ml Preservation solution.

[0050] Will β 2 -MG coated magnetic nanoparticles are uniformly dispersed in the preservation solution.

[0051] Performance test: Use a pipette to accurately pipette 100μl, 0.1mol / LPB6.5 to 1.5mL centrifuge tube, and add 10μl0.05μg / mlβ to it in turn 2 -MG antigen or 0.5μg / mlβ 2 -MG antigen, 20μl of alkaline phosphatase-labeled antibody and 100μl of storage solution dispersed with magnetic nanoparticles, then use a pipette to disperse the mixture evenly and start timing. After 5min, put t...

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Abstract

The invention provides a preserving fluid and its preparation method. The preserving fluid comprises 0.1-10 g/L of buffer salt, 100-300 g/L of an antibody stabilizer, 0.1-1 g/L of an anticorrosive bacteriostatic agent and 2-12 g/L of carragheenan and water. In comparison with the prior art, the invention has the following advantages: the buffer salt, antibody stabilizer and anticorrosive bacteriostatic agent in the preserving fluid maintain stability of magnetic micro-particles or magnetic nano-particles and antibody in the whole system; and viscosity of the carragheenan can decrease exponentially with rising of temperature. Solubility of the carragheenan increases with rising of temperature, molecular dissociation is intensified, electrostatic attraction between half-esterfied sulfate radicals is weakened, and molecular entanglement decreases. Then, viscosity is lowered. But viscosity increases when temperature is reduced. At 30 DEG C, molecules are gradually entangled to form a net structure so as to sharply increase viscosity. Thus, the preserving fluid is in a viscous or gelatinous state at the temperature of 2-20 DEG C but is in a fluid state at the operating temperature of 35-38 DEG C.

Description

technical field [0001] The invention belongs to the field of chemiluminescence immunoassay, in particular to a preservation solution and a preparation method thereof. Background technique [0002] Chemiluminescence immunoassay (Chemiluminescenceimmunoassay, CLIA) is to combine the chemiluminescence system with the immune reaction, label the antibody or antigen with chemiluminescence-related substances, react with the antigen or antibody to be tested, and separate the free chemiluminescence markers, Other related substances added to the chemiluminescence system produce chemiluminescence for quantitative or qualitative detection of antigens or antibodies. Since its development in the 1970s, chemiluminescence immunoassay has become a mature and advanced ultra-trace active substance detection technology. Due to its advantages of high sensitivity, strong specificity, wide detection range, simple operation, high degree of automation, good reagent stability, and very little pollut...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/553
Inventor 冯瑾
Owner SINOCARE
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