Preserving fluid and preparation method thereof

A technology of preservation solution and carrageenan, which is applied in the field of chemiluminescence immunoassay, can solve the problems of affecting test results, unfavorable stable storage of markers on particles, and affecting antibody immune response
CN105300966AInactive Publication Date: 2016-02-03SINOCARE
8 Cites 29 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SINOCARE
Publication Date
2016-02-03
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a preserving fluid and its preparation method. The preserving fluid comprises 0.1-10 g / L of buffer salt, 100-300 g / L of an antibody stabilizer, 0.1-1 g / L of an anticorrosive bacteriostatic agent and 2-12 g / L of carragheenan and water. In comparison with the prior art, the invention has the following advantages: the buffer salt, antibody stabilizer and anticorrosive bacteriostatic agent in the preserving fluid maintain stability of magnetic micro-particles or magnetic nano-particles and antibody in the whole system; and viscosity of the carragheenan can decrease exponentially with rising of temperature. Solubility of the carragheenan increases with rising of temperature, molecular dissociation is intensified, electrostatic attraction between half-esterfied sulfate radicals is weakened, and molecular entanglement decreases. Then, viscosity is lowered. But viscosity increases when temperature is reduced. At 30 DEG C, molecules are gradually entangled to form a net structure so as to sharply increase viscosity. Thus, the preserving fluid is in a viscous or gelatinous state at the temperature of 2-20 DEG C but is in a fluid state at the operating temperature of 35-38 DEG C.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of chemiluminescence immunoassay, in particular to a preservation solution and a preparation method thereof. Background technique

[0002] Chemiluminescence immunoassay (Chemiluminescenceimmunoassay, CLIA) is to combine the chemiluminescence system with the immune reaction, label the antibody or antigen with chemiluminescence-related substances, react with the antigen or antibody to be tested, and separate the free chemiluminescence markers, Other related substances added to the chemiluminescence system produce chemiluminescence for quantitative or qualitative detection of antigens or antibodies. Since its development in the 1970s, chemiluminescence immunoassay has become a mature and advanced ultra-trace active substance detection technology. Due to its advantages of high sensitivity, strong specificity, wide detection range, simple operation, high degree of automation, good reagent stability, and very little pollut...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More