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A method for extracting and isolating pseudophyllum sapogenin from pseudophyllum

A technology of pseudophyllum saponin and pseudophyllum saponin, which is applied to the preparation of plant compound monomers, extraction and separation of pseudophyllum saponin, and the field of pseudophyllum saponin extraction from pseudophyllum saponin, which can solve the problem of increasing coronary flow and enhancing Myocardial contractility, high cost and other problems, to achieve the effect of reducing production cost, optimizing extraction steps, and easy operation

Active Publication Date: 2017-09-19
JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ruscogenin (ruscogenin), also known as ruscogenin, is mainly derived from the dry tuber root of Ophiopogon Japonicus (Thunb.) Ker-Gawl. A variety of steroidal saponins: Ophiopogon japonicus saponins A, B, C, and D all have the effects of anti-myocardial infarction, enhancing myocardial contractility, and increasing coronary artery flow. , can reduce the levels of NO and MDA in serum and tissues, protect various tissues from free radical attacks, have more obvious effects on the heart and lungs and down-regulate the expression of TF genes in lung tissues, and have a good medicinal prospect, but due to the ruthlessness in the original plant Skogenin seldom exists in the form of prototype, but it exists in the form of saponin, which affects its efficacy
[0004] Existing literature is seldom aimed at the extraction and separation of pseudophylla saponin, and conventional methods such as solvent extraction and crystallization are mainly used to separate and purify pseudophytonin monomer, which has low efficiency, low output, unstable process, and high cost. high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The method includes the following steps:

[0028] Take 10 kg of false leaf tree raw material, put it in a container after crushing, add 60 L of methanol with a volume fraction of 70%, heat and reflux for extraction 4 times, each extraction time is 2 hours, and combine the extracts; The speed passes through the macroporous adsorption resin column equipped with 100g of treatment, after the adsorption is completed, it is eluted with 80% methanol, and the eluate is collected, and concentrated under reduced pressure with a thin film at a vacuum of 10.7kPa and a temperature of 70-90°C. until there is no solvent, then filter and dry to obtain a dry product; add 25 mL of 5% sulfuric acid to the dry product, reflux for 3 hours to obtain a hydrolysis mixture, let cool to room temperature, filter, and collect the solid; put the solid in a container, add 5% sodium hydroxide solution , adjusted to neutrality, with solid mass: chloroform volume being 1Kg: 8L, adding chloroform for ex...

Embodiment 2

[0030] Take 50kg of false leaf tree raw material, put it in a container after crushing, add 400L of ethanol with a volume fraction of 80%, heat and reflux for extraction 3 times, each extraction time is 2.5 hours, and combine the extracts; extract the extracts at 0.13BV / min The speed passes through the macroporous adsorption resin column equipped with 100g of treated, after the adsorption is completed, it is eluted with 90% methanol or ethanol, and the eluate is collected, and the film is used for decompression at a vacuum degree of 10.7kPa and a temperature of 70-90°C. Concentrate until there is no solvent, then filter, and dry to obtain a dry product; add 20 mL of 8% sulfuric acid to the dry product, and reflux for 3 hours to obtain a hydrolyzed mixture, let it cool to room temperature, filter, and collect the solid; put the solid in a container, add 5% hydroxide Potassium solution, adjusted to neutral, with solid mass: chloroform volume of 1Kg: 8L, add chloroform for extract...

Embodiment 3

[0032]Take 100kg of false leaf tree raw material, put it in a container after pulverization, add 1000L of ethanol with a volume fraction of 90%, heat and reflux for extraction 3 times, each extraction time is 3 hours, and combine the extracts; extract the extracts at 0.08BV / min The speed passes through the macroporous adsorption resin column equipped with 100g of treatment, after the adsorption is completed, it is eluted with 80% methanol, and the eluate is collected, and concentrated under reduced pressure with a thin film at a vacuum of 10.7kPa and a temperature of 70-90°C. until there is no solvent, then filter and dry to obtain a dry product; add 25 mL of 5% sulfuric acid to the dry product, reflux for 3 hours to obtain a hydrolysis mixture, let cool to room temperature, filter, and collect the solid; put the solid in a container, add 5% sodium hydroxide solution , adjusted to neutrality, with solid mass: chloroform volume is 1Kg: 10L consumption, add chloroform extraction,...

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Abstract

The invention discloses a method for extracting ruscogenin from a pseudo-leaf tree. The method for extracting and separating ruscogenin from a pseudo-leaf tree comprises the following steps: extracting total saponins, absorbing with a macroporous resin, vacuum degree of 10.7kPa, temperature Membrane concentration at 70-90°C, acid hydrolysis, chloroform extraction, silica gel column purification, and recrystallization. The method provided by the invention is easy to operate, short in production cycle, high in separation efficiency, stable in process, and low in cost, and can realize the high-purity separation and preparation of industrialized pseudophylla saponin monomer, and the purity of the obtained pseudophylla saponin monomer reaches More than 98%, the recovery rate of active ingredients is greater than 85%.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of active components of traditional Chinese medicine, and relates to a preparation method of a plant compound monomer, in particular to a method for extracting and separating pseudophyllum saponin, and in particular to a method for extracting pseudophyllum saponin from pseudophyllum saponin Methods. Background technique [0002] Ruscus aculeatus, a shrub belonging to the genus Ruscus aculeatus in the Asparagaceae family, likes warm, humid and sunny environments. Native to Eurasia, Western Europe and the Mediterranean coast. The branches and leaves of false leaves are dark green, and they are often cultivated as foliage plants. They are decorated in living rooms and halls. Another medical study has proved that the extract of the pseudoleaf tree can stimulate the production of vascular endothelial growth factor for the treatment of hair disorders, especially hair tufts and male...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00
Inventor 冒玉娟王莹王华赵丽
Owner JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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