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A selenium-enriched Candida glabrata strain fxy-4 and its cultivation method and application as a fish feed additive

A technology of Candida glabrata and FXY-4 is applied in the field of microorganisms to achieve the effects of enhancing immunity, increasing added value and improving economic benefits

Active Publication Date: 2018-09-25
广州邹鲁农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of high-yield selenium-enriched Candida glabrata in aquaculture feed has not been reported

Method used

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  • A selenium-enriched Candida glabrata strain fxy-4 and its cultivation method and application as a fish feed additive
  • A selenium-enriched Candida glabrata strain fxy-4 and its cultivation method and application as a fish feed additive
  • A selenium-enriched Candida glabrata strain fxy-4 and its cultivation method and application as a fish feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Candida glabrata FXY-4, which can be obtained as follows:

[0021] Isolation of the original strain:

[0022] Dilute the pickle juice sample and spread it on the plate of YEPD medium, cultivate it at 28°C, observe the growth state of the colony at regular intervals, and pick a single colony and transfer it to the YEPD slant for storage. After preliminary screening, the following yeasts are obtained: Strains: FXY-1, FXY-3, FXY-4, FXY-5, FXY-7, FXY-10, FXY-11, FXY-19, FXY-21, FXY-23, FXY-24, FXY-25, FXY-38, FXY-42, FXY-43, FXY-44, FXY-46, FXY-47, FXY-49, FXY-50, FXY-53, FXY-55, FXY-56.

[0023] containing Na 2 SeO 3 (final concentration is 0.5g / L) in the YEPD substratum that carries out the screening of selenium-enriched yeast, then the yeast that preliminary screening obtains is cultivated with YEPD substratum, and every strain bacterium is divided into blank group and experimental group and cultivates, Add Na to the experimental group 2 SeO 3 (final concentration ...

Embodiment 2

[0025] Sequence Analysis of 18S ITS rRNA Gene of Yeast Strain FXY-4 (Deposit Number: CCTCC NO: M 2015664):

[0026] (1) Extraction of chromosomal DNA (small amount):

[0027] 1. Take 1.5mL bacterial liquid in a 1.5mL Eppendorf tube and centrifuge at 12 000r / min for 5min;

[0028] 2. Discard the supernatant, resuspend the pellet in 900 μL phosphate buffered solution (PBS), and centrifuge at 12 000 r / min at 4 °C for 5 min;

[0029] 3. Discard the supernatant, add 300TE and 200μL 10mg / mL lysozyme, blow and aspirate to mix, incubate at 37°C for 1h, and mix by inverting every 15min;

[0030] 4. Add 600TENS lysate (200mmol / L NaCl, 100mmol / L Tril-HCl pH8.0, 2.0% SDS, 50mmol / L EDTA, 0.5% Triton X-100) and 20mg / mL proteinase K 10μL to the pellet, mix by inversion , incubate at 55°C for 1 hour, and mix by inverting every 15 minutes;

[0031] 5. Centrifuge at 12 000r / min at 5.4°C for 5min, and take the supernatant;

[0032]6. Add an equal volume of P:C:I (25:24:1) to the supernatant,...

Embodiment 3

[0060] Yeast FXY4 (Deposit number: CCTCC NO: M 2015664), Bio Mérieux VITEK 2 automatic identification analysis

[0061] Pick the bacterial lawn of the strain to be tested and inoculate it on the YEPD plate, cultivate it to the logarithmic growth phase at 30°C, dip the Vitek liquid in the kit with a sterilized cotton swab, scrape off the bacterial lawn on the plate, and then dissolve it in 1.8ml Vitek liquid , oscillate and mix well; use a turbidimeter to adjust the turbidity to the specified range (2MacF); then use a pipette gun to add the bacterial suspension to the Vitek identification plate, and the bacterial solution is well filled with the identification hole; then incubate at 30°C for 24 to 48 hours When the identification plate was taken out, the identification plate was read and analyzed with the Bio Mérieux VITEK 2 automatic identification analyzer. The results are shown in Table 3. The physiological and biochemical results are the same as the typical physiological and...

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Abstract

The invention relates to a selenium-rich candida glabrata strain FXY-4 as well as a cultivation method and an application of selenium-rich candida glabrata strain FXY-4 serving as an additive for fish feed and belongs to the technical field of microorganisms. A pickle juice sample is diluted, a YEPD (yeast extract peptone dextrose) culture medium is coated with the pickle juice sample for cultivation, single colonies are picked out, selenium-rich saccharomycete is screened through the YEPD culture medium containing Na2SeO3 with the final concentration being 0.5g / L, and the selenium-rich candida glabrata strain FXY-4 is obtained with a chemical mutagenesis method and classified and named as candida glabrata FXY-4, has been preserved in CCTCC (China Center For Type Culture Collection) and has the preservation number CCTCC NO:M 2015664. The selenium-rich candida glabrata FXY-4 has remarkable immunity enhancing and growth promoting functions, and an animal experiment proves that the selenium-rich candida glabrata strain FXY-4 has a promoting function on growth and health of aquatic animals and can be used as the additive to be applied to the fish feed.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular, the invention relates to a selenium-enriched Candida glabrata strain FXY-4, a cultivation method thereof and an application as a fish feed additive. Background technique [0002] Selenium (Se) is an essential trace element for animals and plays an important role in maintaining animal health, but excessive selenium can cause toxicity to the body, so research on the optimal amount of selenium in animal feed has been extensively carried out. Nutritional research on selenium in fish feed shows that too high or too low selenium content in feed will affect the health of fish. In the process of intensive aquaculture, some pathogenic bacteria, especially intestinal conditional pathogens, can multiply rapidly and cause corresponding diseases. At present, the main method is to control and reduce stress by adding antibiotics in the feed. Antibiotic supplementation can inhibit the gro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02A23K10/18A23K50/80C12R1/72
Inventor 代俊陈雄张毅俞灿王志李欣周梦舟唐景峰朱莹
Owner 广州邹鲁农业有限公司
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