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Rapid culture method and culture medium of ureaplasma urealyticum

A technology of Ureaplasma urealyticum and culture medium, which is applied in the field of biological culture, can solve the problems of long interpretation time, unfavorable disease diagnosis and treatment, etc., and achieve the effects of reliable detection, shortened detection time, and improved accuracy

Inactive Publication Date: 2016-02-17
刘慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it takes a long time to interpret the results of the solid culture method, so it takes a long time to diagnose the urogenital tract infection caused by UU and MH, which is not conducive to the diagnosis and treatment of the disease

Method used

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  • Rapid culture method and culture medium of ureaplasma urealyticum
  • Rapid culture method and culture medium of ureaplasma urealyticum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The configuration of embodiment 1 culture medium

[0013] Culture medium preparation: There are 5 groups in total, including medium 1-4 four groups and control 1 group. Control group 1 was mycoplasma broth solid medium.

[0014] Medium 1 is 12g of agar powder, 10g of pure fresh beef extract powder, 3g of HEPES buffer, 0.5g of L-cysteine, 5g of potassium dihydrogen phosphate, 1.5g of urea, 0.2g of manganese sulfate, and 1g to 3g of glucose , horse serum 300ml, antibiotic 700,000IU, sulfamethoxazole 3g, trimethoprim 5mg, polymyxin 6mg, RPMI1640 cell culture medium 5g formula configuration, distilled water to volume 1000ml.

[0015] Medium 2 is 12g of agar powder, 10g of pure fresh beef extract powder, 3g of HEPES buffer, 0.5g of L-cysteine, 5g of potassium dihydrogen phosphate, 1.5g of urea, 0.2g of manganese sulfate, and 1g to 3g of glucose. Horse serum 300ml, arginine 2g, antibiotic 700,000IU, trimethoprim 5mg, polymyxin 6mg, RPMI1640 cell culture medium 5g, and disti...

Embodiment 2

[0018] Embodiment 2 medium culture effect verification

[0019] Specimen collection and culture method:

[0020] Ten cases of urogenital swabs from gynecological patients were all positive for Ureaplasma urealyticum, and were divided into 5 groups through expansion culture, with 3 parallel samples in each group. Mycoplasma hominis specimens served as controls. The solid medium was inoculated with swabs respectively, then cultured at 36°C, and the culture results were checked after 10 hours.

[0021] Result Judgment (Color)

[0022]

[0023]

Embodiment 3

[0024] Embodiment 3 culture medium culture accuracy verification

[0025] Take 10 cases of clinical specimens, inoculate solid medium 1, then culture at 36°C, and test the culture results after 10 hours. It was found through testing that 4 cases were positive for Ureaplasma urealyticum.

[0026] Through the accuracy identification of the Ureaplasma urealyticum PCR detection kit in CN102409098A, all 4 cases were found to be positive for Ureaplasma urealyticum, and the results were accurate. Ureaplasma urealyticum was not detected in the remaining 6 cases.

[0027] Conclusion: Medium 1 can effectively improve the accuracy of Ureaplasma urealyticum specimen testing, reduce the occurrence of false negatives and false positives, and further strengthen the correct rate of clinical diagnosis of gynecological patients.

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Abstract

The invention discloses a rapid culture method and a culture medium of ureaplasma urealyticum. Compared with a culture medium in the prior art, the culture medium disclosed by the invention has the characteristic that multiple components are added and have effects of rapidly improving the culture efficiency and effectively identifying the ureaplasma urealyticum. The rapid culture method and the culture medium have the advantages of short culture time and easiness in identification.

Description

technical field [0001] The invention relates to a rapid culture method for Ureaplasma urealyticum and a culture medium thereof, belonging to the field of biological culture. Background technique [0002] Ureaplasma (Ureaplasma) is one of 13 species of mycoplasma isolated from the human body that can multiply by itself. Ureaplasma is between viruses and bacteria, and is the smallest prokaryotic microorganism that can reproduce on artificial medium. It has no cell wall, can replicate itself, and is club-shaped, with a size of 125-250nm and a molecular weight of 4.5XIO8 Highly pleomorphic. Ureaplasma is divided into two biological groups and 14 serotypes according to molecular biological characteristics. The two biological groups include Ureaplasma parvum (Up) and Ureaplasma urealyticum (Uu). Among the 14 serotypes , serotypes 1, 3, 6, and 14 with smaller genomes belong to ureaplasma microbes, accounting for 90% to 92% of ureaplasma infections; serotypes 2, 4, 5, 8 to 13 with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 刘慧陈艳萍代玉龙辛克盛
Owner 刘慧
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