Kit for rapidly detecting various viruses in muskmelon by one step and rapid detection method adopting kit
A melon yellow spot virus and kit technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of few types of melon virus diseases, and achieve low equipment requirements and high accuracy. , the effect of fewer steps
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment approach 1
[0039] Specific embodiment one: the kit for the rapid detection of multiple viruses in muskmelon contains 7 pairs of RT-PCR primers in this embodiment;
[0040] A pair of primers for amplifying watermelon mosaic virus disease: the nucleotide sequence of the upstream primer WMV1F is 5'-GAGGAACTGTGGTTCATGTC-3', and the nucleotide sequence of the downstream primer WMV1R is 5'-GCAGTGTGCCTCTCAGTATT-3';
[0041] A pair of primers for amplifying cucumber mosaic virus disease: the nucleotide sequence of the upstream primer CMV1F is 5'-GACAGTTGGGAATCGGA-3', and the nucleotide sequence of the downstream primer CMV1R is 5'-AACAGGGAGCAAGAGGA-3';
[0042] A pair of primers used to amplify the yellow mosaic virus of zucchini: the nucleotide sequence of the upstream primer ZYMV-F is 5'-CACGAAGGACAAGGATGTGA-3', and the nucleotide sequence of the downstream primer ZYMV-R is 5'-ACATTGCTAAGAGCTGCTGC-3 ';
[0043] A pair of primers for amplifying squash mosaic virus: the nucleotide sequence of t...
specific Embodiment approach 2
[0047] Specific embodiment two: present embodiment one step rapid detection muskmelon multiple virus carries out according to the following steps:
[0048] 1. Extract the total RNA of infected virus leaves;
[0049] 2. Reverse transcription into cDNA;
[0050] 3. Perform multiple RT-PCR with 7 pairs of RT-PCR primers in the kit to determine whether the leaves are infected with watermelon mosaic virus, cucumber mosaic virus, zucchini yellow mosaic virus, squash mosaic virus, Tobacco mosaic virus, papaya ringspot virus and melon yellow spot virus, and virus types;
[0051] In step 3, 7 pairs of RT-PCR primers are primer pairs for amplifying watermelon mosaic virus: the nucleotide sequence of the upstream primer WMV1F is 5'-GAGGAACTGTGGTTCATGTC-3', and the nucleotide sequence of the downstream primer WMV1R is 5 '-GCAGTGTGCCTCTCAGTATT-3';
[0052] A pair of primers for amplifying cucumber mosaic virus disease: the nucleotide sequence of the upstream primer CMV1F is 5'-GACAGTTGG...
specific Embodiment approach 3
[0060] Embodiment 3: The difference between this embodiment and Embodiment 2 is that in Step 2, random primers and reverse transcriptase are used for reverse transcription. Other steps and parameters are the same as those in Embodiment 2.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com