Application of a kind of barley aglycone and its derivatives for preventing and treating skin proliferative diseases
A technology of derivatives and aglycones, applied in the field of biomedicine, can solve the problems of low cure rate, inability to effectively prolong the remission period of patients, slow curative effect, etc., and achieve the effects of good safety, good application prospect and remarkable effect.
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Embodiment 1
[0045] Example 1 Effects of barley aglycone and its derivatives on the viability of human epidermal keratinocytes
[0046] Human epidermal keratinocytes HaCaT cells were seeded into 96-well plates and cultured at a cell density of 1.2×10 4 1 / ml, dosing after 12h, the drugs were all diluted with 1640 medium containing 3% FBS, and the negative group was used as a control with 1640 medium containing 3% FBS containing DMSO. The volume is 100 μl / well; after the cells are cultured in a 37°C incubator containing 5% CO2 for 44 hours, add 20 μl MTT (5 mg / ml) to each well and continue to culture for 4 hours; discard the supernatant, add 100 μl DMSO to each well, On the instrument, vibrate for 10 minutes, detect the OD value at 570nm, and calculate the inhibition rate, inhibition rate=(1-absorbance mean value of the experimental group / absorbance mean value of the control group)×100%; independent experiments were repeated more than three times, and the experimental data were all used IC5...
Embodiment 2
[0050] Example 2 Detection of the Non-toxic Dose of Glycoside and Its Derivatives to Subcutaneous Connective Tissue Cells
[0051] Inoculate A9 subcutaneous connective tissue cells in 96-well plates with a cell density of 1.2×10 4 Individual / ml; Dosing after 12h, the drugs were all diluted with DMEM medium containing 3% FBS, and the negative group was used as a control with DMEM medium containing 3% FBS containing DMSO. The volume is 100 μl / well; after the cells were cultured for 44 hours in a 37°C incubator containing 5% CO2, 20 μl of MTT (5 mg / ml) was added to each well, and the culture was continued for 4 hours; On the standard instrument, vibrate for 10 minutes, and detect the OD value at 570nm; the independent experiment was repeated more than three times, and the experimental data were all calculated with SPSS statistical software. The results are shown in Table 2, which indicated that the aglycone and 579-1 to 579-27 derivatives had no obvious toxicity to subcutaneous ...
Embodiment 3
[0055] Example 3 Effects of barley aglycone and its derivatives on DNA synthesis in HaCaT cells
[0056] In order to further explore the effects of barley aglycone and its derivatives on the proliferation of HaCaT cells, we conducted a BrdU incorporation experiment. The HaCaT cells were treated with agluconin and its derivatives at a final concentration of 2 μg / ml, and the DMSO treatment group was used as a negative control. After adding the drug for different times, after adding BrdU for 6 hours, after a series of treatments, use a microplate reader at 450nm Check its OD value. see results figure 1 , the BrdU incorporation of HaCaT cells treated with saliva aglycone was lower than that of the DMSO control group at 12h, 24h, 36h and 48h, and reached a very significant level at 24h, indicating that saliva aglycone can inhibit HaCaT DNA synthesis of cells to inhibit their proliferation. Other derivatives also showed similar results.
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