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Molecular marker Insert 144 of soybean epidemic-disease-resistant gene RpsQ and application of molecular marker Insert 144

A molecular marker and disease resistance technology, applied in the fields of agricultural biotechnology engineering and crop disease resistance genetics and breeding, can solve the problems of complex population structure of Phytophthora sojae, loss of resistance of single-gene resistant varieties, etc., to improve breeding efficiency, accurate The effect of high sex and speeding up the work process

Inactive Publication Date: 2016-02-24
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The population structure of Phytophthora sojae is complex, and it is easy to produce new virulence types
Emergence of new virulence forms of Phytophthora sojae leading to loss of resistance in monogenic resistant varieties

Method used

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  • Molecular marker Insert 144 of soybean epidemic-disease-resistant gene RpsQ and application of molecular marker Insert 144
  • Molecular marker Insert 144 of soybean epidemic-disease-resistant gene RpsQ and application of molecular marker Insert 144
  • Molecular marker Insert 144 of soybean epidemic-disease-resistant gene RpsQ and application of molecular marker Insert 144

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 is used to illustrate the acquisition of the candidate gene of soybean blight resistance gene RpsQ and its co-segregated molecular marker Insert144

[0032] 1. Genetic analysis of resistance in Qichadou 1

[0033] The disease-resistant variety Qichadou No. 1 (the variety approved by the Crop Research Institute of Shandong Academy of Agricultural Sciences in 1995, formerly code-named Ji 30) was used as the male parent, and the susceptible variety Jikedou No. The horn DNA was introduced into the Jilin No. 30 variety, and the mutant strain was selected from the offspring, and bred) as the female parent, and crossed to produce F 1 Generation, F 1 F 2 Generation, F 2 210 F 2:3 Families are used for resistance genetic analysis, disease resistance gene identification and mapping populations. Using the hypocotyl wound inoculation method, 20-25 plants per family were identified for their response to Phytophthora sojae strain Ps41-1. After inoculation, at 23-25° C...

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Abstract

The invention discloses a molecular marker Insert 144 closely chained with a soybean epidemic-disease-resistant gene RpsQ. A nucleotide sequence of the molecular marker Insert 144 is shown as SEQ ID No.1, and a genetic distance between the molecular maker and the soybean epidemic-disease-resistant gene RpsQ is 0 cM. Detection analysis shows that the molecular marker can accurately track the soybean epidemic-disease-resistant gene RpsQ and predict epidemic-disease-resistant characteristics of soybean, so that convenience is brought to molecular design breeding. The invention further discloses a method for identifying the soybean epidemic-disease-resistant gene RpsQ. By utilizing the method, quick identifying detection of soybean epidemic-disease-resistant plants can be realized.

Description

technical field [0001] The invention belongs to the fields of agricultural biotechnology engineering and crop disease-resistant genetic breeding, and specifically relates to a molecular marker Insert144 of a soybean disease-resistant gene RpsQ and an application thereof. Background technique [0002] Soybean blight caused by Phytophthorasojae Kaufmann & Gerdemann is a devastating disease in soybean production. At present, the annual economic loss caused by soybean blight is about 1 billion US dollars worldwide (Tyler, 2007). [0003] The most economical, effective, and environmentally safe way to control soybean blight is to plant disease-resistant varieties. Soybean resistance to blight is characterized by qualitative traits and quantitative traits. Quality trait resistance is controlled by a single gene. At present, 25 resistance genes have been mapped to different chromosomes. [0004] The population structure of Phytophthora sojae is complex, and it is easy to produc...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/11C12Q1/68A01H5/00
Inventor 朱振东
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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