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RGD-labeled fluorescent gold nano-cluster preparation method

A technology of fluorescent gold nanometers and gold nanoclusters, applied in preparations for in vivo experiments, medical preparations of non-active ingredients, pharmaceutical formulations, etc., achieving low cytotoxicity, good application prospects and economic value, and simple and easy synthesis methods The effect of the operation

Inactive Publication Date: 2016-03-02
屈晓超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technique of the RGD-labeled fluorescent gold nanocluster prepared by the present invention has not yet been reported, especially the method of using RGD as a fluorescent nanoprobe targeting ligand has not been reported.

Method used

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  • RGD-labeled fluorescent gold nano-cluster preparation method
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  • RGD-labeled fluorescent gold nano-cluster preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of gold nanoclusters (AuNCs@BSA):

[0041] Prepare chloroauric acid aqueous solution (10mM, 5mL) and BSA aqueous solution (50mg·mL -1 , 5mL); the aqueous solution of chloroauric acid was added to the BSA aqueous solution, and stirred vigorously at a constant temperature of 37 degrees Celsius for 30 minutes; the preparation of NaOH aqueous solution (1M, 0.5mL) was added to the mixed solution of chloroauric acid BSA after 30 minutes, and the Stirring was continued for 12 hours; the reacted product was dialyzed in deionized water for 12 hours, and then centrifuged with a 10kDa ultrafiltration tube.

Embodiment 2

[0043] Preparation of gold nanoclusters (AuNCs@BSA):

[0044] Prepare chloroauric acid aqueous solution (10mM, 5mL) and BSA aqueous solution (50mg·mL -1 , 5mL); the aqueous solution of chloroauric acid was added to the BSA aqueous solution, and stirred vigorously at a constant temperature of 37 degrees Celsius for 30 minutes; the preparation of NaOH aqueous solution (1M, 0.5mL) was added to the mixed solution of chloroauric acid BSA after 30 minutes, and the Stirring was continued for 16 h; the reacted product was dialyzed in deionized water for 12 h, and then centrifuged with a 10 kDa ultrafiltration tube.

Embodiment 3

[0046] Preparation of gold nanoclusters (AuNCs@BSA):

[0047] Prepare chloroauric acid aqueous solution (10mM, 5mL) and BSA aqueous solution (50mg·mL -1 , 5mL); the aqueous solution of chloroauric acid was added to the BSA aqueous solution, and stirred vigorously at a constant temperature of 37 degrees Celsius for 30 minutes; the preparation of NaOH aqueous solution (1M, 0.5mL) was added to the mixed solution of chloroauric acid BSA after 30 minutes, and the Stirring was continued for 24 hours; the reacted product was dialyzed in deionized water for 12 hours, and then centrifuged with a 10kDa ultrafiltration tube.

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Abstract

The present invention discloses a RGD-labeled fluorescent gold nano-cluster preparation method, which comprises: preparing gold nanoparticles with a particle size of less than 2 nm by using a BSA method; and carrying out an amidation reaction on the prepared gold nano-cluster and RGD having amino to obtain the RGD-labeled fluorescent gold nano-clusters. According to the present invention, the obtained gold nano-cluster (Au Nanoclusters, AuNCs) is the novel precious metal fluorescent nanometer material, and has characteristics of simple preparation conditions, simple preparation steps, near-infrared light emitting under a visible light source, small particle size, good monodispersity, good stability, low toxic-side effect, high safety, stable fluorescent property, and high CT imaging contrast resolution; a small amount of expression of integrin [alpha]v[beat]3 exists in most normal tissues and mature vascular endothelial cells while the over-expression of the integrin [alpha]v[beat]3 exists in a variety of cancer cells and neovessel endothelial cells, and the ligand is called RGD polypeptide sequence; and the RGD is selected as the targeting molecule, and the AuNCs and the RGD polypeptide sequence are conjugated to prepare the RGD-labeled fluorescent gold nano-cluster RGD-AuNCs@BSA.

Description

technical field [0001] The present invention relates to a method for preparing a nano-preparation for fluorescence / CT dual-mode imaging and capable of targeting tumor cells, and further relates to the application of such composite multifunctional nanoparticles as fluorescent probes and CT imaging contrast agents in the field of biomedicine application. Background technique [0002] In order to improve the diagnosis and treatment of tumors, targeted tumor imaging and drug delivery have become important medical methods. [0003] CT imaging uses different tissues or organs to attenuate X-rays to different degrees due to their different densities and thicknesses, and forms gray-scale image contrast distribution maps of different tissues or organs, so as to determine the relative position, shape and size of the lesion and other changes to judge the medical imaging method of the disease. CT imaging technology has the advantages of high spatial resolution, short image acquisition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/04A61K47/48A61K49/00
Inventor 屈晓超李奕辰李蕾王彦然梁敬宁梁继民
Owner 屈晓超
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