Molecular marker method of main effect qtl locus qrr5 of rooting rate of catalpa cuttage

Abstract

The invention provides a molecular marking method for the major QTL locus qRR5 for the rooting rate in cuttage of catalpa bungei and belongs to the field of molecular genetics. 70 pairs of SSR molecule markers are used for determining gene types of 87 parts of clonal materials of catalpa bungei, and whole genome association linkage analysis is carried out with the combination of the rooting rate in cuttage of catalpa bungei. The major QTL locus qRR5 for the rooting rate in cuttage of catalpa bungei is detected, 13.35% of phenotypic variation is explained, and the SSR molecular marker CB252 is highly remarkably associated with phenotypic variation. The molecular marking method is beneficial to solving the problem of the slow progress of catalpa bungei breeding in China and beneficial to solving the technical problem that the existing breeding technology is high in cost, long in time, poor in stability and the like when applied to increasing the rooting rate in cuttage of catalpa bungei, and breeding of new varieties of catalpa bungei in China and clone forestry development can be accelerated.

Description

technical field

[0001] The invention provides a molecular marker method for the main effect QTL site qRR5 of the rooting rate of catalpa cuttings, belongs to the field of molecular genetics, and is specially used for directional cultivation of new species of catalpa cuttings that are easy to root. Background technique

[0002] Catalpa ( Catalpa bungei ) belongs to Bignoniaceae ( Bignoniaceae ) Catalpa ( Catalpa ) is an excellent and precious timber tree species. With the development of industry and the growth of the world population, the demand for wood is increasing day by day, and the shortage of wood has become a worldwide problem, especially in my country. The development of clonal forestry has become an effective way to alleviate the shortage of timber in my country, but the rooting rate of cuttings is the bottleneck restricting the development of clonal forestry. Catalpa is a tree species with low cutting rooting rate, and the difficulty of cutting rooting limits...

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