Fluorescence detection instrument and system based on micro-fluidic chip

A microfluidic chip and fluorescence detection technology, applied in the direction of instruments, fluorescence/phosphorescence, analytical materials, etc., can solve the problems of not taking into account volume, resolution and sensitivity, and can not meet the requirements, so as to achieve low processing and manufacturing costs and easy detection , The effect of simple system design

Inactive Publication Date: 2016-03-09
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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Problems solved by technology

[0006] However, the current microfluidic chip fluorescence detection devices for immunoassays cannot take into account the volume, resolution a

Method used

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  • Fluorescence detection instrument and system based on micro-fluidic chip
  • Fluorescence detection instrument and system based on micro-fluidic chip
  • Fluorescence detection instrument and system based on micro-fluidic chip

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Example Embodiment

[0035] Example 1

[0036] The functional layer of the microfluidic chip 11 selected in this embodiment is mainly composed of a cuboid channel and a conical inlet, the channel width is 500 microns, and the height is 500 microns. The optical filter 3 selected in this embodiment is the FF-05 optical filter in the center of the Daheng Optical Film; the fluorescent substance on the microfluidic chip 11 is FITC, its molecular weight is 389.4, and its maximum absorption wavelength is 490-495nm. The emitted light wavelength is 525-530nm; the selected light source 1 is an LED light source; the selected fluorescence detection module 6 is a CCD camera; the selected microfluidic chip support structure 7 is a chip tray. Cover the longitudinal microfluidic pipeline on the substrate of the microfluidic chip 11, pass through the A / B / C / D / E pipelines into the AFP (alpha-fetoantigen) capture antibody, suck it out after 30 minutes, and remove the vertical microfluidic pipeline; Cover the horizon...

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Abstract

The invention provides a fluorescence detection instrument and system based on a micro-fluidic chip. The instrument comprises an instrument frame, a light source, a micro-fluidic chip supporting structure, a fluorescence collection module, a light path fixing module and a fluorescence detection module, wherein the light source, the micro-fluidic chip supporting structure, the fluorescence collection module, the light path fixing module and the fluorescence detection module are installed on the instrument frame, the light source is used for stimulating a fluorescent substance on the chip to emit fluorescent light, the micro-fluidic chip supporting structure is used for supporting and moving the chip, and the fluorescence collection module is used for filtering, collecting and focusing a fluorescence signal on the micro-fluidic chip onto the fluorescence detection module; the light path fixing module is used for fixing the position of the fluorescence collection module and for light path adjustment, and the fluorescence detection module is used for converting a received optical signal into an analog electrical signal and then converting the analog electrical signal into a digital electrical signal. The instrument is connected with a computer to form a system. The instrument can meet the requirement for fluorescence detection on the micro-fluidic chip. The instrument is high in resolution ratio and sensitivity, simple and compact in structure, small in size, and convenient to carry and use.

Description

technical field [0001] The invention relates to the technical field of microfluidic chip analysis, in particular to a microfluidic chip-based fluorescence detection instrument and system. Background technique [0002] Microfluidic analysis method is a newly developed technology in recent years. This method is mainly based on analytical chemistry, combined with the achievements of biochemistry, molecular biology, physical chemistry, immunology and other related disciplines. Nano-liter to pico-liter volume fluid can be used to analyze and detect the substances on the side. It has the advantages of small volume, large specific surface area, short reaction time, fast analysis speed, less reagent and sample consumption, and simultaneous detection of multiple samples and multiple indicators. The discovery of disease markers and early diagnosis of diseases in research have important practical value. [0003] Conventional diagnostic analysis methods require a relatively long analys...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N35/00
Inventor 蒋兴宇沈海滢张伟
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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