Fluorescence detection instrument and system based on micro-fluidic chip

[0035] Example 1

[0036] The functional layer of the microfluidic chip 11 selected in this embodiment is mainly composed of a cuboid channel and a conical inlet, the channel width is 500 microns, and the height is 500 microns. The optical filter 3 selected in this embodiment is the FF-05 optical filter in the center of the Daheng Optical Film; the fluorescent substance on the microfluidic chip 11 is FITC, its molecular weight is 389.4, and its maximum absorption wavelength is 490-495nm. The emitted light wavelength is 525-530nm; the selected light source 1 is an LED light source; the selected fluorescence detection module 6 is a CCD camera; the selected microfluidic chip support structure 7 is a chip tray. Cover the longitudinal microfluidic pipeline on the substrate of the microfluidic chip 11, pass through the A/B/C/D/E pipelines into the AFP (alpha-fetoantigen) capture antibody, suck it out after 30 minutes, and remove the vertical microfluidic pipeline; Cover the horizontal microfluidic pipeline, after the pipeline is sealed by bovine serum albumin solution, add AFP antigen 0ng/mL, 20ng/mL, 40ng/mL, 80ng/mL, 160ng to the 1/2/3/4/5 pipeline in sequence /mL, after incubating for 30 minutes, add the cleaning solution, FITC-labeled AFP detection antibody, and cleaning solution in sequence; place the microfluidic chip 11 on the chip tray of the instrument, push the tray to the designated position, and turn on the detection and analysis function of the instrument Software, read the fluorescent signal, the test results are as follows image 3 shown. It can be seen from the figure that as the concentration of AFP antigen increases, the intensity of the fluorescent signal increases gradually.