The invention discloses a seed soaking device for agricultural seedling culture, and belongs to the field of agriculture. The seed soaking device comprises a seed soaking box, a water inlet pipe is arranged on the side wall of the seed soaking box, a telescopic rod is arranged on the lower surface of a horizontal plate, the bottom end of the telescopic rod is connected with a fixed circular ring,a seed soaking mesh basket is detachably arranged on the fixed circular ring, an ultraviolet sterilizing lamp is mounted on the inner wall of the seed soaking box, an inclined guide plate is arrangedat the bottom end of the seed soaking box, a water discharging pipe is arranged at the lowest end of the inclined guide plate, a heater is mounted on the inclined guide plate, a pesticide adding pipeis arranged on the seed soaking box, one end of a spraying pipe is connected with the water inlet pipe, a plastic hose is arranged at the other end of the spraying pipe, and a spraying disk is arranged on the plastic hose. According to the seed soaking device, agricultural seeds are soaked underwater and cannot be floated on the water surface, proper water temperature and water flowing are ensuredin the seed soaking process, seed soaking efficiency and effect is improved, breeding and propagating of harmful bacteria on the seeds are avoided, eggs on the seeds are killed, safe seedling cultureof the seeds can be ensured, the germination rate of the seeds is increased, and growth of the seeds is facilitated.