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Composite accelerator for Cordyceps cultivation and preparation method thereof

A compound accelerator, Cordyceps technology, applied in the directions of botanical equipment and methods, biocides, animal repellants, etc., can solve the problems of Cordyceps yield limitation, long cultivation period, low cordycepin content, etc., to promote the growth of Cordyceps, Low cost and the effect of increasing economic benefits

Active Publication Date: 2018-01-23
河南仁添生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The growth and development of Cordyceps requires high-efficiency nutrients. At present, rice is used as the main raw material to make the medium, and the output of Cordyceps is limited. The content of cordycepin in Cordyceps is low, and the cultivation period is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Inoculate the spore suspension of mucormyces CICC 3115 in YPG liquid medium sterilized at 115°C for 15 minutes. The culture temperature is 30°C, the initial pH is 6.0, the rotation speed is 150r / min, and the time is 60h.

[0022] (2) After the mucor culture is completed, the mycelium is filtered out and cleaned, and then the filtered mycelium is frozen and ultrasonically crushed. The ratio of mycelium weight to water volume (m / v) is 1:5, and the ultrasonic crushing condition is 30s each time, 60s interval, 30 cycles, after the hyphae are broken, let stand at 35°C for 6h to obtain the broken self-solution.

[0023] (3) Add octacosanol, inositol, and fructooligosaccharides (1 to 3 fructosyl groups through β(2—1) glycosidic bonds and kestose) and copper gluconate were mixed uniformly to obtain a embryo material composite accelerator; among them, octacosanol was added according to the mass volume ratio of the mucor mycelia broken self-solution at 1%, and inositol Add a...

Embodiment 2

[0026] (1) Inoculate the spore suspension of mucormyces CICC 3115 in YPG liquid medium sterilized at 115°C for 15 minutes. The culture temperature is 30°C, the initial pH is 6.0, the rotation speed is 150r / min, and the time is 60h.

[0027] (2) After the mucormycetes culture is over, filter out the mycelium and wash it. Then freeze and sonicate the filtered mycelia. The ratio of mycelium weight to water volume (m / v) is 1:4. , Standing for 6h, obtained broken self-solution.

[0028] (3) Add octacosanol, inositol, fructooligosaccharides (kestose), and copper gluconate to the mucor broken self-solution obtained in step (2) and mix evenly to obtain a composite accelerator for blank material; Wherein octacosanol is added according to the mass volume ratio 1.5% of the self-solution broken with Mucor mycelium, inositol is added according to the mass volume ratio of 3.0% of the self-solution broken with Mucor mycelia, and fructooligosaccharide is added according to the mass volume ra...

Embodiment 3

[0031](1) Inoculate the spore suspension of mucormyces CICC 3115 in YPG liquid medium sterilized at 115°C for 15 minutes. The culture temperature is 30°C, the initial pH is 6.0, the rotation speed is 150r / min, and the time is 60h.

[0032] (2) After the mucormycetes culture is over, filter out the mycelium and wash it. Then freeze and sonicate the filtered mycelia. The ratio of mycelium weight to water volume (m / v) is 1:3. , Standing for 6h, obtained broken self-solution.

[0033] (3) Add octacosanol, inositol, fructooligosaccharides (kestose), and copper gluconate to the mucor broken self-solution obtained in step (2) and mix evenly to obtain a composite accelerator for blank material; Wherein octacosanol is added according to the mass volume ratio 2.0% of the self-solution broken with Mucor mycelium, inositol is added according to the mass volume ratio of 4.5% of the self-solution broken with Mucor mycelium, and fructooligosaccharide is added according to the mass volume ra...

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Abstract

The invention discloses a compound promoter for cordyceps sinensis cultivation and a preparation method thereof, and belongs to the technical field of agriculture plantation. The embryo compound promoter required for growth of cordyceps sinensis is prepared by conducting compounding through a hairy mold mycelium crushing self solution, inositol, octacosanol, fructo-oligosaccharide and copper gluconate. By means of the embryo compound promoter, the growth cycle of cordyceps sinensis can be remarkably shortened, the cordyceps sinensis yield is increased, the weight of cordyceps sinensis in the unit volme is increased, the content of cordycepin of the compound promoter is remarkably increased, and the processing method is simple, low in cost, capable of being widely used and capable of enhancing economic benefits of enterprises.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a cultivation embryo material composite accelerator for rapid and high-efficiency cultivation technology of Cordyceps and a preparation method thereof. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, or Cordyceps for short, belongs to the genus Cordyceps ergotaceae of the Ascomycetes Hypocreales order. Cordyceps is divided into stroma, mycelium, and fruiting body. The fruiting body is rich in a variety of essential amino acids, beneficial trace elements and vitamins and other nutrients, as well as adenosine, cordycepin, cordycepic acid, cordyceps polysaccharide, superoxide dismutation Active ingredients such as enzymes (SOD) have various pharmacological functions such as anti-tumor, anti-pathogen, anti-oxidation, and enhancing human immunity. Therefore, it is of great significance to develop and utilize fungal resour...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N31/06A01N31/02A01N43/16A01N55/02A01P21/00
CPCA01N31/02A01N31/06A01N43/16A01N55/02A01N59/16A01N63/30A01N2300/00
Inventor 汪超刘璐李冬生卢忠诚刘婷石勇
Owner 河南仁添生物科技有限公司
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