A kind of tumor naked-eye visible nano-tracer and its preparation method

A tracer and naked-eye technology, applied in the field of medicine, can solve the problems of difficult large-scale industrial production and poor stability, and achieve the effects of increased contact area, enhanced adhesion ability, and small particle size

Active Publication Date: 2019-06-07
NANNING KELUN NEW TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nanoliposomes have limitations: poor stability and difficult large-scale industrial production

Method used

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  • A kind of tumor naked-eye visible nano-tracer and its preparation method
  • A kind of tumor naked-eye visible nano-tracer and its preparation method
  • A kind of tumor naked-eye visible nano-tracer and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A naked-eye visible nano-tracer for tumors, comprising the following raw materials:

[0058] Soluble protein: serum albumin 5mg;

[0059] Lecithin: 40mg;

[0060] Staining agent: Genipin GP5 mg;

[0061] The method for preparing the above-mentioned naked-eye visible nano-tracer for tumors comprises the following steps:

[0062] (1) Add lecithin into cyclohexane, stir and dissolve to make a lecithin solution with a concentration of 1.6%;

[0063] (2) Add the dye to ultrapure water, stir to dissolve, and make a dye solution with a concentration of 7%;

[0064] (3) Add soluble protein into ultrapure water, stir and dissolve to make a protein solution with a concentration of 1%, mix it with the lecithin solution obtained in step (1) at a volume ratio of 1:15, oscillate and sonicate for 12 minutes, and make protein lecithin mixture;

[0065] (4) Mix the protein-lecithin mixture obtained in step (3) with the dye solution obtained in step (2) at a volume ratio of 15:1, os...

Embodiment 2

[0070] A naked-eye visible nano-tracer for tumors, comprising the following raw materials:

[0071] Soluble protein: rice protein 50 mg;

[0072] Lecithin: 300mg;

[0073] Staining agent: methylene blue dipropionate NHS ester 50 mg;

[0074] The method for preparing the above-mentioned naked-eye visible nano-tracer for tumors comprises the following steps:

[0075] (1) Add lecithin into n-hexane, stir and dissolve to make a lecithin solution with a concentration of 10%;

[0076] (2) Add the dye to ultrapure water, stir to dissolve, and make a dye solution with a concentration of 20%;

[0077] (3) Add soluble protein into ultrapure water, stir and dissolve to make a protein solution with a concentration of 10%, put it into a homogenizer for 10 minutes with the lecithin solution obtained in step (1) at a volume ratio of 2:15 , to make protein lecithin mixture;

[0078] (4) Put the protein lecithin mixture solution obtained in step (3) and the dye solution obtained in step (...

Embodiment 3

[0083] A naked-eye visible nano-tracer for tumors, comprising the following raw materials:

[0084] Soluble protein: silkworm chrysalis water-soluble protein 30 mg, soybean protein 10 mg;

[0085] Lecithin: 200 mg;

[0086] Staining agent: methylene blue succinate NHS lipid 15 mg, loganin aglycon LA 5 mg;

[0087] The method for preparing the above-mentioned naked-eye visible nano-tracer for tumors comprises the following steps:

[0088] (1) Add lecithin into n-heptane, stir and dissolve to make a lecithin solution with a concentration of 8%;

[0089] (2) Add the dye to ultrapure water, stir to dissolve, and make a dye solution with a concentration of 12%;

[0090] (3) Add soluble protein into ultrapure water, stir and dissolve to make a protein solution with a concentration of 5%, and put it into a high-speed dispersing machine at a volume ratio of 1:15 to disperse for 15 minutes with the lecithin solution obtained in step (1). Make protein lecithin mixture;

[0091] (4)...

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Abstract

The invention provides a tumor naked eye visible nanometer tracer agent. The tumor naked eye visible nanometer tracer agent is prepared from 5-50 parts of soluble protein, 40-300 parts of lecithin and 5-50 parts of dye. The blue tumor naked eye visible nanometer tracer agent with the grain size smaller than or equal to 100 nm is prepared through ultrapure water dissolution and even mixing. The tumor naked eye visible nanometer tracer agent has biological compatibility and biodegradability and is safe and free of toxin for human bodies, and surgeons can distinguish tumor tissue and normal tissue through naked eyes under normal light rays without the help of additional instruments and accurately cut off tumors in surgery. A preparation method of the biodegradability is suitable for industrialized large-scale production.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a naked-eye visible nanometer tracer for tumors and a preparation method thereof. Background technique [0002] Tumor tissue is easily distinguished from normal tissue by preoperative examinations such as MRI, CT, and ultrasound, but it is difficult to distinguish them with the naked eye during surgery. It is difficult to completely remove cancerous tissue in general surgery, leaving hidden dangers for disease recurrence. If the scope of resection is expanded, normal tissues will be damaged, which may lead to sequelae in terms of body function and function. Therefore, distinguishing normal cells from tumor tissues has always been a difficult problem in related fields. [0003] In the past, imaging techniques such as MRI, CT, and ultrasound required special instruments, and it was difficult to directly observe tumors; tumor fluorescence staining methods needed to be excited by l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 聂国朝吴艳华张东伟朱潜荣
Owner NANNING KELUN NEW TECH CO LTD
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