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Total saponin extract of marsdenia tenacissima and extraction method thereof

A technology of clearing vine glycosides and an extraction method, which is applied in the field of total saponin extract of clearing vine, and can solve the problems of large dosage, large difference in components, and difficulty in controlling known active ingredients and the like.

Active Publication Date: 2016-03-23
SHANXI PROVINCE CHINESE MEDICINE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the main usage of Tongguan rattan medicinal materials is simple boiling, boiling alcohol precipitation or extraction with a certain concentration of ethanol, without enriching the medicinal ingredients in it, resulting in an increase in the content of many invalid ingredients (including ingredients that cannot be absorbed by the human body). overdose
At the same time, due to the large difference in ingredients between medicinal materials, it is difficult to control the content of known active ingredients in crude extracts made by simple processes, which causes inconvenience in the quality control of crude extracts and affects the curative effect.

Method used

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  • Total saponin extract of marsdenia tenacissima and extraction method thereof
  • Total saponin extract of marsdenia tenacissima and extraction method thereof
  • Total saponin extract of marsdenia tenacissima and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Get 10Kg of Tengguan Teng medicinal material, place in 100Kg of 70% ethanol solution, soak overnight, heat to reflux temperature for extraction, the number of times of extraction is 3 times, each 2 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure to recover ethanol, and then freeze-dried to obtain about 800 g of the crude extract of Tengguanteng.

[0057] Soak ZTC-1 macroporous adsorption resin with 2 times the volume of acetone overnight, place it in a chromatography column, rinse with acetone until there is no white precipitate, then rinse with ethanol until the ethanol solution is added with water without white turbidity, then rinse with distilled water Rinse until alcohol-free.

[0058] MCIGel reversed-phase resin for the same treatment.

[0059] Dissolve the crude extract of Tengguan vine with 12Kg distilled water, centrifuge, take the supernatant and slowly add it to the ZTC-1 macroporous adsorption resin column, the flow ...

Embodiment 2

[0063] Get 10Kg of Tengguan vine medicinal material, place in 120Kg of 70% ethanol solution, soak overnight, heat to reflux temperature for extraction, the number of times of extraction is 3 times, each 2 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure to recover ethanol, and then freeze-dried to obtain about 850 g of the crude extract of Tengguanteng.

[0064] Soak ZTC-1 macroporous adsorption resin with 2 times the volume of acetone overnight, place it in a chromatography column, rinse with acetone until there is no white precipitate, then rinse with ethanol until the ethanol solution is added with water without white turbidity, then rinse with distilled water Rinse until alcohol-free.

[0065] MCIGel reversed-phase resin for the same treatment.

[0066] Dissolve the crude extract of Tengguan vine with 17Kg distilled water, centrifuge, take the supernatant and slowly add it to the ZTC-1 macroporous adsorption resin column, control t...

Embodiment 3

[0070] Get 10Kg of Tengguan vine medicinal material, place in 80Kg of 75% ethanol solution, soak overnight, heat to reflux temperature for extraction, the number of times of extraction is 3 times, each 2 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure to recover ethanol, and then freeze-dried to obtain about 720 g of the crude extract of Tengguanteng.

[0071] Soak ZTC-1 macroporous adsorption resin with 2 times the volume of acetone overnight, place it in a chromatography column, rinse with acetone until there is no white precipitate, then rinse with ethanol until the ethanol solution is added with water without white turbidity, then rinse with distilled water Rinse until alcohol-free.

[0072] MCIGel reversed-phase resin for the same treatment.

[0073] Dissolve the crude extract of Tengguan vine with 14.4Kg distilled water, centrifuge, take the supernatant and slowly add it to the ZTC-1 macroporous adsorption resin column, control ...

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Abstract

Marsdenia tenacissima total saponin. 1 mg of the marsdenia tenacissima total saponin includes 0.3-0.4 mg of tenacissoside A, 0.1-0.2 mg of marsdekoiside K, 0.02-0.03 mg of isotenacissoside A, 0.02-0.03 mg of tenacissoside aglycon B and 0.1-0.2 mg of tenacissoside G. The isotenacissoside A is represented as the formula (I) and is an isomeride of the tenacissoside A. The marsdenia tenacissima total saponin includes various anti-tumor active components. Compared with a chemical therapy drug, the marsdenia tenacissima total saponin has the anti-tumor effects and meanwhile has a significant effect on recovery of body autoimmunity functions.

Description

technical field [0001] The invention relates to an extract of total saponins of vine vine, which contains a new compound of vine glucoside. The present invention also relates to the preparation method of the total saponin extract of the vine vine glycoside containing the new vine glucoside compound. Background technique [0002] The incidence of malignant tumors is increasing and has a high mortality rate, which seriously threatens human health. The use of radiotherapy and chemotherapy to treat malignant tumors not only kills cancer cells, but also damages normal cells of the body, and the prognosis is not ideal. Cisplatin injection, a broad-spectrum anti-cancer drug commonly used in clinic, has obvious killing ability on tumor cells, but also has strong immunosuppressive effect, resulting in low immunity of the body during treatment and relatively large side effects. At present, it is urgent to find an anti-cancer drug with obvious anti-tumor effect but less damage to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00A61P35/00A61K36/27
CPCA61K36/27A61K2236/333A61K2236/55C07J71/001
Inventor 李媛媛郝旭亮倪艳岳永花李先荣贺石麟程玉钏刘聪郭丁丁
Owner SHANXI PROVINCE CHINESE MEDICINE RES INST
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