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A kind of polyamide-amine hyperbranched gene carrier and its preparation method and application

A gene carrier and polyamide technology, applied in the field of polyamide-amine hyperbranched gene carrier and its preparation, can solve the problems of poor buffer capacity, poor branching degree, high raw material cost, etc., and achieve good buffer capacity, low cost, and method simple effect

Inactive Publication Date: 2020-11-06
SHANGHAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current cationic polymer polyamide-amine has poor branching degree, high toxicity when used as a gene carrier, poor buffering capacity, and high raw material cost

Method used

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  • A kind of polyamide-amine hyperbranched gene carrier and its preparation method and application
  • A kind of polyamide-amine hyperbranched gene carrier and its preparation method and application
  • A kind of polyamide-amine hyperbranched gene carrier and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A polyamide-amine hyperbranched gene carrier, its raw materials include A material, B material, C material and triethylamine;

[0034] The molecular formula of A material is: C 2(n-1) h 5n-2 N n , where n=2,3,4,5;

[0035] The structural formula of C material is: where n=0,1,2,3,4,5,6, R=C m h 2m+1 , m=1,2,3,4,5;

[0036] B material structure formula is: n=1,2,3,4,5,6; or

[0037] x=y=1,2,3,4,5,6.

Embodiment 2

[0039] A polyamide-amine hyperbranched gene carrier, its raw materials include N, N'-methylenebisacrylamide, L-cysteine ​​methyl ester hydrochloride, triethylamine, triethylenetetramine and water.

[0040] Material A can be ethylenediamine, triethylenetetramine or polyethylene polyamine with a molecular weight of 250-300.

[0041] The molar ratio of N, N'-methylenebisacrylamide to L-cysteine ​​methyl ester hydrochloride is 3:2.4, and the molar ratio of N, N'-methylenebisacrylamide to triethylenetetramine 1:80. The weight-to-volume ratio g:ml of N,N'-methylenebisacrylamide and solvent water is 0.8:4.

Embodiment 3

[0043] A polyamide-amine hyperbranched gene carrier, its raw materials include N,N'-methylenebisacrylamide, L-cysteine ​​methyl ester hydrochloride, triethylamine, polyethylene polyamine with a molecular weight of 275 and solvents.

[0044] The molar ratio of N,N'-methylenebisacrylamide to L-cysteine ​​methyl ester hydrochloride is 2.5:2.9, and the molar ratio of N,N'-methylenebisacrylamide to polyethylene polyamine 1:120. The weight-to-volume ratio g:ml of N,N'-methylenebisacrylamide to the solvent is 0.2:8. The solvent includes 1 part of water and 5 parts of DMSO by volume.

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PUM

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Abstract

The invention discloses a polyamid-amine hyperbranched gene carrier. The gene carrier is prepared from a material A, a material B, a material C and triethylamine. The molecular formula of the material A is C2(n-1)H(5n-2)Nn, wherein n is equal to 2, 3, 4 or 5. Please see the structural formula of the material C in the specification, wherein n is equal to 0, 1, 2, 3, 4, 5 or 6, R is equal to the sum of CmH2m+1, and m is equal to 1, 2, 3, 4 or 5. Please see the structural formula of the material B in the specification, wherein n is equal to 1, 2, 3, 4, 5 or 6; or please see the other structural formula of the material B in the specification, wherein x and y are equal to 1, 2, 3, 4, 5 or 6. The invention further discloses a preparation method and application of the polyamid-amine hyperbranched gene carrier. The polyamid-amine hyperbranched gene carrier is good in branching degree and is small in toxicity and good in buffering capacity when used as gene carriers, the polyamid-amine hyperbranched gene carrier can be used for anti-cancer treatment, raw material cost is low, and the preparation method is simple.

Description

technical field [0001] The invention relates to the technical field of polyamide-amine gene carrier, in particular to a polyamide-amine hyperbranched gene carrier and its preparation method and application. Background technique [0002] With the rapid development of synthetic chemistry, many new molecular structures can be designed to explore the topology of polymers. Hyperbranched polymers have become a current research hotspot due to their unique structure and physical and chemical properties; hyperbranched polymers are widely used in biological Sensing, phase separation, battery energy, gene delivery, drug delivery, hydrogel, photocatalyst carrier and biomedicine, etc., due to the special three-dimensional structural characteristics of hyperbranched polymers, can be synthesized by two divergent and convergent methods. Because of their unique physical and chemical properties, such as low viscosity, high solubility, high compatibility, and high reactivity, hyperbranched pol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/87C08G83/00A61K48/00A61P35/00
CPCA61K48/0041C08G83/006C12N15/87
Inventor 邓维张薇刘梦艳洪诗斌姚子健张建勇周龙
Owner SHANGHAI UNIV
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