Method for introducing lactate intercalated LDH (layered double hydroxide) ultrathin nanosheet loaded bioactive molecules into plant cells

Abstract

The invention discloses a method for introducing lactate intercalated LDH (layered double hydroxide) ultrathin nanosheet loaded bioactive molecules into plant cells. The method comprises the following steps: a lactate intercalated LDH nanomaterial MgAl-lactate LDH is synthesized and delaminated in water, and a two-dimensional nanosheet LDH-lactate-NS is obtained; the LDH-lactate-NS is subjected to characterization analysis and identification including XRD (X-ray diffraction) and the like; culture media with different LDH-lactate-NS concentration are used for treating plant material BY-2 cells and Arabidopsis thaliana, and safe concentration allowing introduction of the LDH-lactate-NS loaded bioactive molecules into the plant cells is obtained; the bioactive molecules and the LDH-lactate-NS are coupled in different mass ratios, and a nanosheet-bioactive molecule compound with the optimal adsorption ratio is obtained; the nanosheet-bioactive molecule compound with the optimal adsorption ratio is co-incubated with the plant cells, and the bioactive molecules are introduced into complete living plant cells within the optimal incubating time.

Description

technical field [0001] The invention belongs to the technical field of biologically active molecules, and relates to a method for importing biologically active molecules into plant cells by lactate-intercalated hydrotalcite-like ultrathin nanosheets loaded with biologically active molecules. Background technique [0002] Bioactive molecule introduction technology is still limited by high efficiency, economy and ease of operation in terms of introducing acidic bioactive molecules into plant cells, and is still a bottleneck for real-time detection of living cells in a wider range of plant species one. Related studies have reported that researchers used materials such as porous silicon nanoparticles to introduce DNA molecules into plant protoplasts. However, when preparing protoplasts, not only is the operation cumbersome and the yield is low, but also the obtained protoplasts are very fragile and easily broken. Therefore, researchers have been exploring an ideal carrier that...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for importing bioactive molecules into plant cells by lactate-intercalated hydrotalcite-like ultra-thin nanosheets, which solves the problem that it is difficult to introduce bioactive molecules into intact living plant cells in the prior art

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